The dried blood spot (DBS) sampling has a lot of advantages in comparison with the “standard” venous collecting, such as small collection volume, painless and easy sample minimal training required, stable transportable at ambient temperatures, etc. aim this study was to determine comparability four different types DBS (HemaSpot™-HF Blood Collection Device, Whatman® 903 Protein Saver Snap Apart Card, card ImmunoHealth™, glass fiber strip ImmunoHealth™) for analysis global metabolites profile....

Scientists currently use only a small portion of the information contained in blood metabolome. The identification metabolites is huge challenge because highly abundant and well-separated compounds can be easily identified complex samples. However, new approaches that enhance have emerged; among them, based on their involvement particular biological context recent development. In this work, approach was first applied to identify samples and, together with metabolite set enrichment analysis,...

Lung cancer is one of the most common types in men, and a leading cause cancer-related deaths. Therefore, identification specific markers associated with risk lung development, particularly metabolites that are more easily assayed, would be very valuable. To this end, а comparative metabolomics study blood plasma samples collected from patients (n=100) controls recruited Moscow was carried out. After extraction proteins methanol, remaining metabolite fractions were analyzed directly using...

The goal of this study was to evaluate the capacity for mass spectrometry blood plasma diagnose impaired glucose tolerance (IGT). For study, samples from control subjects (n = 30) and patients with IGT 20) were treated methanol low molecular weight fraction then analyzed by direct infusion spectrometry. A total 51 metabolite ions strongly associated detected. area under a receiver operating characteristic (ROC) curve (AUC) diagnosing that based on an analysis all these metabolites 0.93...

In metabolomics, a large number of small molecules can be detected in single run. However, metabolomic data do not include the absolute concentrations each metabolite. Generally, mass spectrometry analyses provide metabolite that are derived from peak intensities, and intensities strictly dependent on type spectrometer used, as well technical characteristics, options protocols applied. To convert to actual concentrations, calibration curves have generated for metabolite, this represents...

Background: Recent decades have been marked by advances in omics sciences based on high-throughput technologies, which enabled the measurement of enormous numbers molecules biosamples. In metabolomics, a large number small (metabolome) can be detected single run. The goal this study was to evaluate capacity for metabolomic analysis blood plasma early-stage Parkinson Disease (PD) diagnosis. Methods: Blood samples collected from control subjects (n = 20) and patients with PD (Hoehn Yahr stages...

Diabetic nephropathy (DN) is one of the specific complications diabetes mellitus and leading kidney-related disorders, often requiring renal replacement therapy. Currently, tests commonly used for diagnosis DN, albuminuria (AU) glomerular filtration rate (GFR), have limited sensitivity specificity can usually be noted when typical morphological changes in kidney already been manifested. That why extreme urgency problem early this disease exists. The untargeted metabolomics analysis blood...

In metabolomics, many metabolites are measured simultaneously in a single run. Such analytical performance opens up prospects for clinical laboratory diagnostics. this work, mass spectrometric metabogram was developed as simplified and clinically applicable way of measuring the blood plasma metabolome. To develop metabogram, samples from healthy male volunteers (n = 48) approximately same age, direct infusion spectrometry (DIMS) low molecular fraction samples, principal component analysis...

The clinical blood metabogram (CBM) was developed to match a tailored analysis of the metabolome time, cost, and reproducibility constraints laboratory testing. By analyzing main metabolite groups, CBM offers clinically relevant information about intake low-molecular substances into organism, humoral regulation, liver function, amino acid level, lipid carbohydrate metabolism. purpose this work investigate relevance using in patients with diabetes mellitus. For this, obtained for 18 healthy...

A new method for the analysis of blood lipid based on direct mass spectrometry lipophilic low molecular weight fraction plasma has been considered. Such technique allows quantification hundreds various types lipids and this changes existing concepts diagnostics disorders related diseases. The versatility quickness significantly simplify its wide use. This is applicable atherosclerosis, diabetes, cancer other Detalization composition at level by means to assess effectiveness therapy optimize...

A laboratory-developed test (LDT) is a type of in vitro diagnostic that designed, manufactured and used the same laboratory (i.e., an in-house test). In this study, metabolomics-based LDT was developed. This involves blood plasma preparation, direct-infusion mass spectrometry analysis with high-resolution spectrometer, alignment normalization peaks using original algorithms, metabolite annotation by biochemical context-driven algorithm, detection overrepresented metabolic pathways results...

Recently, the concept of a mass spectrometric blood metabogram was introduced, which allows analysis metabolome in terms time, cost, and reproducibility clinical laboratory tests. It demonstrated that components are related groups metabolites associated with humoral regulation; metabolism lipids, carbohydrates, amines; lipid intake into organism; liver function, thereby providing clinically relevant information. The purpose this work to evaluate relevance using disease. To do this, used...

The article describes a new therapeutic drug monitoring (TDM) method based on direct infusion of low-molecular fraction blood into electrospray ionization source mass spectrometer. This technique allows performing TDM almost all drugs used in clinic. In article, the universality and high-throughput method, that significantly simplifies its wide application, have been shown. Moreover, possibility application most cases therapy has argued as tool control doses, rationality therapy, quality...

In metabolomics, thousands of substances can be detected in a single assay. This capacity motivates the development metabolomics testing, which is currently very promising option for improving laboratory diagnostics. However, simultaneous measurement an enormous number leads to data often representing concentrations only conditional units, while diagnostics generally require actual concentrations. To convert concentrations, calibration curves need generated each substance, and this process...

Early diagnostics significantly improves the survival of patients with renal cell carcinoma (RCC), which is prevailing type adult kidney cancer. However, absence clinically obvious symptoms and effective screening strategies at early stages result to disease progression rate reducing. The study was focused on revealing potential low molecular biomarkers for early-stage RCC. untargeted direct injection mass spectrometry-based metabolite profiling blood plasma samples from 51 non-cancer...

In terms of time, cost, and reproducibility clinical laboratory tests, a mass spectrometric blood metabogram (CBM) enables the investigation metabolome. Metabogram’s components provide clinically relevant information by describing related groups metabolites connected to humoral regulation, metabolism lipids, carbohydrates amines, lipid intake into organism, liver function. For further development CBM approach, ability detect metabolic changes in early stages Parkinson’s disease (PD) was...

Fish species exhibit great diversity rating of aging (from negligible to rapid), which gives a unique possibility for the discovery molecular mechanisms that determine differences in rate aging. A mass spectrometric metabolic profiling skeletal muscle fish with various rates was carried out by direct injection quadrupole time-of-flight spectrometer. The first group includes long-lived (pike (Esox Lucius) and sterlet (Acipenser ruthenus); second group—species gradual senescence such as...

The increase in life expectancy, leading to a rise the proportion of older people, is accompanied by prevalence age-related disorders among world population, fight against which today one biomedical challenges. Exploring biological insights concerning lifespan ways provide background for designing an effective treatment healthy years life. Untargeted direct injection mass spectrometry-based metabolite profiling 12 species Drosophila with significant variations natural lifespans was conducted...

Recently, a clinical blood metabogram was developed as fast, low-cost and reproducible test that allows the implementation of metabolomics in practice. The components are functionally related groups metabolites associated with humoral regulation, metabolism lipids, carbohydrates amines, lipid intake into organism, liver function, thereby providing clinically relevant information. It is known gut microbiota affects metabolome, metabolome may affect composition microbiota. Therefore, before...