Wnt-11 is a secreted protein that modulates cell growth, differentiation and morphogenesis during development. We previously reported expression elevated in hormone-independent prostate cancer the progression of from androgen-dependent to androgen-independent proliferation correlates with loss mutual inhibition between Wnt-11- androgen receptor-dependent signals. However, prevalence increased patient tumours functions cells were not known. levels determined by immunohistochemical analysis...

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a member of the betacoronavirus family, which causes COVID-19 disease. SARS-CoV-2 pathogenicity in humans leads to increased mortality rates due alterations significant pathways, including some resulting exacerbated inflammatory responses linked “cytokine storm” and extensive lung pathology, as well being number comorbidities. Our current study compared five sequences from different geographical regions those SARS, MERS two cold...

Breast cancer (BCa) is one of the leading health problems among women. Although significant achievements have led to advanced therapeutic success with targeted therapy options, more efforts are required for different subtypes tumors and according genomic, transcriptomic, proteomic alterations. This study underlines role microRNA-21 (miR-21) in metastatic MDA-MB-231 breast cells. Following knockout miR-21 from cells, which highest expression levels compared MCF-7 SK-BR-3 BCa a decrease...

Abstract Androgen deprivation therapy (ADT) is the core treatment for advanced prostate cancer (PCa), with a proven survival benefit. ADT lowers circulating testosterone levels throughout body, but it comes variety of reported side effects including fatigue, muscle wastage, weight gain, hot flushes and importantly cognitive impairment, depression, mood swings. Testosterone has key role in brain masculinization, its direct are relatively poorly understood, due both to brain’s extreme...

Glioblastoma multiforme (GBM) is the most aggressive form of adult primary malignant brain tumour with poor prognosis. Extracellular vesicles (EVs) are a key-mediator through which GBM cells promote pro-oncogenic microenvironment. Peptidylarginine deiminases (PADs), catalyze post-translational protein deimination target proteins, implicated in cancer, including via EV modulation. Pan-PAD inhibitor Cl-amidine affected release from cells, and related microRNA cargo, reduced microRNA21...

Glioblastoma multiforme (GBM) is the most common and aggressive form of primary malignant brain tumor in adults, with poor prognosis. Extracellular vesicles (EVs) are key-mediators for cellular communication through transfer proteins genetic material. Cancers, such as GBM, use EV release drug-efflux, pro-oncogenic signaling, invasion immunosuppression; thus modulation cargo considerable clinical relevance. As EV-inhibitors have been shown to increase sensitivity cancer cells chemotherapy, we...

Glioblastoma multiforme (GBM) is an aggressive adult brain tumour with poor prognosis. Roles for peptidylarginine deiminases (PADs) in GBM have recently been highlighted. Here, two cell lines were treated PAD2, PAD3 and PAD4 isozyme-specific inhibitors. Effects assessed on extracellular vesicle (EV) signatures, including EV-microRNA cargo (miR21, miR126 miR210), changes cellular protein expression relevant mitochondrial housekeeping (prohibitin (PHB)) cancer progression (stromal interaction...

Pancreatic ductal adenocarcinoma (PDAC) is the most common and aggressive type of pancreatic cancer (PCa) with a low survival rate. microRNAs (miRs) are endogenous, non-coding RNAs that moderate numerous biological processes. miRs have been associated chemoresistance metastasis PDAC presence subpopulation highly plastic "stem"-like cells within tumor, known as stem (CSCs). In this study, we investigated role miR-21, which expressed in Panc-1 MiaPaCa-2 association CSCs. Following miR-21...

Glycogen synthase kinase (GSK-3) is upregulated in many types of tumor, including prostate cancer. GSK-3 inhibitors reduce tumor cell growth; however, it not clear if both isoforms, GSK-3α and GSK-3β, are involved. Here, we compared their expression tumors used gene silencing to study functions 22Rv1 cancer cells. Compared normal prostate, GSK-3β were 25/79 24/79 cases cancer, respectively, with elevated low Gleason sum score expressed high tumors, isoforms correlating the androgen receptor...

Prostate cancer (PCa) is the second-leading cause of cancer-related death among men. microRNAs have been identified as having potential roles in tumorigenesis. An oncomir, miR-21, commonly highly upregulated many cancers, including PCa, and showed correlation with Wnt-signaling axis to increase invasion. Wnt-11 a developmentally regulated gene has found be but its mechanism unknown. The present study aimed investigate miR-21 PCa vivo vitro. First, different Gleason score tissue samples were...

The identification of biomarkers for early diagnosis Parkinson’s disease (PD) is pivotal importance improving approaches clinical intervention. use translatable animal models pre-motor PD therefore offers optimal opportunities novel biomarker discovery in vivo. Peptidylarginine deiminases (PADs) are a family calcium-activated enzymes that contribute to protein misfolding through post-translational deimination arginine citrulline. Furthermore, PADs an active regulator extracellular vesicle...

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive malignancies with limited survival rate. Roles for peptidylarginine deiminases (PADs) have been studied in relation to a range cancers roles epigenetic regulation (including histone modification and microRNA regulation), cancer invasion, extracellular vesicle (EV) release. Hitherto though, knowledge on PADs PDAC limited. In current study, two cell lines (Panc-1 MiaPaCa-2) were treated pan-PAD inhibitor Cl-amidine as well...

Abstract The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage‐gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation EGF for 24 h more than doubled VGSC current density. Similar treatment significantly dose‐dependently enhanced the cells' migration through Transwell filters. Both patch clamp recordings assay suggested that endogenous played...

Peptidylarginine deiminases (PADs) are a family of calcium-regulated enzymes that phylogenetically conserved and cause post-translational deimination/citrullination, contributing to protein moonlighting in health disease. PADs implicated range inflammatory autoimmune conditions, the regulation extracellular vesicle (EV) release, their roles infection immunomodulation known some extent, including viral infections. In current study we describe putative for COVID-19, based on silico analysis...

Extracellular vesicles (EVs) are released from cells and carry protein genetic cargo involved in cell communication. EVs isolated bodyfluids, including plasma serum, can also be used as markers of pathophysiological changes. Peptidylarginine deiminases (PADs) phylogenetically conserved enzymes with physiological roles cause post-translational deimination. This affect function target proteins deimination is linked to EV release. Atlantic cod (Gadus morhua L.) reared at 4 °C 9 respectively for...

Breast cancer (BC) persists as the predominant malignancy globally, standing foremost cause of cancer-related mortality among women. Despite notable advancements in prevention and treatment, encompassing incorporation targeted immunotherapies, a continued imperative exists for development innovative methodologies. These methodologies would facilitate identification women at heightened risk, enhance optimization therapeutic approaches, enable vigilant monitoring emergent treatment resistance....

Abstract Background Although a high level of functional voltage-gated sodium channel (VGSC) expression has been found in strongly metastatic human and rat prostate cancer (PCa) cells, the mechanism(s) responsible for upregulation is unknown. The concentration epidermal growth factor (EGF), modulator ion channels, body highest prostatic fluid. Thus, EGF could be involved VGSC PCa. effects on highly PCa PC-3M cell line, which was shown previously to express both VGSCs receptors, were...