A5086934778- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Genetic and phenotypic traits in livestock
- MicroRNA in disease regulation
- Aquaculture disease management and microbiota
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- Aquaculture Nutrition and Growth
- Genetic Mapping and Diversity in Plants and Animals
- Renal and related cancers
- 3D Printing in Biomedical Research
- Meat and Animal Product Quality
- CRISPR and Genetic Engineering
- Protein Hydrolysis and Bioactive Peptides
- Reproductive Physiology in Livestock
- Epigenetics and DNA Methylation
- Sirtuins and Resveratrol in Medicine
- Reproductive System and Pregnancy
- Chromosomal and Genetic Variations
- Cancer-related molecular mechanisms research
- Ovarian function and disorders
- Italian Fascism and Post-war Society
- Antioxidant Activity and Oxidative Stress
- Pancreatic function and diabetes
- Cephalopods and Marine Biology
University of Milan
2015-2025
Colorado Center for Reproductive Medicine
2016-2024
Colorado State University
2016-2023
Institute of Developmental Physiology
2023
Division of Undergraduate Education
2023
Fondazione Stella Maris
2018
Parco Tecnologico Padano
2015-2017
The objective of this work was to determine the role mitochondria in loss oocyte quality with maternal aging. Our results show that mitochondrial DNA (mtDNA) copy number and function are reduced eggs from aged mice after both vivo vitro maturation. Higher incidences spindle abnormalities were observed old eggs. However, no correlation egg ATP content found. In matured did not have a normal cortical distribution active subject increased oxidative stress due higher levels reactive oxygen...
Background The availability of the bovine genome sequence and SNP panels has improved various genomic analyses, from exploring genetic diversity to aiding selection. However, few on chips are polymorphic in buffalo, therefore a panel single nucleotide DNA markers exclusive for buffalo was necessary molecular analyses develop selection approaches water buffalo. creation 90K river testing wide association study milk production is described here. Methods genomes 73 buffaloes 4 different breeds...
To increase the sustainability of trout farming, industry requires alternatives to fish-based meals that do not compromise animal health and growth performances. develop new feeds, detailed knowledge intestinal morphology physiology is required. We performed histological, histochemical, immunohistochemical morphometric analysis at typical time points in vivo feeding trials (50, 150 500 g). Only minor changes occurred during whereas differences characterized two compartments, linearly...
Chromatin reorganization governs the regulation of gene expression during preimplantation development. However, landscape chromatin dynamics in this period has not been explored bovine. In study, we constructed a genome-wide map accessible bovine oocytes and early embryos using an improved assay for transposase-accessible with high-throughput sequencing (ATAC-seq) which revealed unique features embryo We found that accessibility is low 2-/4-cell embryos, followed by significant increase...
Resveratrol is one of the most investigated natural polyphenolic compounds and contained in more than 70 types plants red wine. The widespread interest this polyphenol derives from its antioxidant, anti-inflammatory anti-aging properties. Several studies have established that resveratrol regulates animal reproduction. However, mechanisms action potential therapeutic effects are still unclear. This review aims to clarify role male female reproductive functions, with a focus on animals...
The challenges posed by climate change and increasing world population are stimulating renewed efforts for improving the sustainability of animal production. To meet such challenges, contribution genomic selection approaches, in combination with assisted reproductive technologies (ARTs), to spreading preserving genetics is essential. largest increase genetic gain can be achieved shortening generation interval. This review provides an overview current status progress advanced ARTs that could...
Abstract Chemically defined oocyte maturation media supplemented with FGF2, LIF, and IGF-1 (FLI medium) enabled significantly improved quality in multiple farm animals, yet the molecular mechanisms behind such benefits were poorly defined. Here, we first demonstrated that FLI medium enhanced mouse assessed by blastocyst formation after vitro fertilization implantation fetal development embryo transfer. We then analyzed glucose concentrations spent media; reactive oxygen species...
Sheep are the most bred species in Central Italy Apennine using natural pastures as a trophic resource and grazing activity is fundamental to maintain grassland biodiversity: this goal can be reached only ensuring an economical sustainability farmers. This study aimed investigate apelin/apelin receptor system ovine mammary gland evaluate differences induced by food supplementation, order shed light on function. A flock of 15 Comisana x Appenninica adult dry ewes were free graze from June...
The ever-increasing number and variation of raw materials utilized to provide alternative feed formulations continues allow for a more sustainable flexible approach. Testing all these options in vivo is still the most robust reliable manner pick best material candidates, but it requires use large numbers animals time-consuming expensive. Therefore, we are developing an vitro platform that can evaluation new ingredients. main aim this work was combine digestion protocol extruded, commercially...
To provide prominent accessibility of fishmeal to the European population, currently available, time- and cost-extensive feeding trials, which evaluate fish feed, should be replaced. The current paper reports on development a novel 3D culture platform, mimicking microenvironment intestinal mucosa in vitro. key requirements model include sufficient permeability for nutrients medium-size marker molecules (equilibrium within 24 h), suitable mechanical properties (G' < 10 kPa), close...
Astaxanthin (AST) is a natural compound derived from shellfish, microorganisms, and algae, with several healthy properties. For this reason, it widely used in the diet of humans animals, such as pigs, broilers, fish, where its addition related to pigmenting Moreover, AST’s ability reduce free radicals protect cells oxidative damage finds application during weaning period, when piglets are exposed stressors. To better elucidate mechanisms involved, here we generate ad hoc pig rainbow trout...
Nerve growth factor (NGF) plays a critical role in reproduction through paracrine and endocrine mechanisms. However, its autocrine effects on uterine receptivity inflammatory pathways remain unknown. This study is the first to demonstrate NGF's direct action sheep endometrial luminal epithelial cells (SELECs), primary cultures treated with NGF for 12, 24, 48 h, or without NTRK1 antagonist. significantly increased PGE2 (p < 0.0001) PGF2α levels only at 12 no significant changes 24 h. also...
Intracytoplasmic spermatid injection into oocytes has limited efficiency in cattle, with no offspring generated so far, partly due to ambiguous identification. This study aimed develop and validate a method for isolating characterizing bovine spermatids improve the of intracytoplasmic injection. First, we optimized protocol isolation from bull testis using discontinuous Percoll gradient 10 μm mesh cell strainers. Next, established stage-specific separation strategy based on DNA content,...
Abstract Further refinement of culture media is needed to improve the quality embryos generated in vitro . Previous results from our laboratory demonstrated that uptake nutrients by embryo significantly less than what supplied traditional media. Our objective was determine impact reduced nutrient concentrations medium on mouse development, metabolism, and as a possible platform for next generation formulation. Concentrations carbohydrates, amino acids, vitamins could be 50% with no...
We derived two novel cell lines from rainbow trout (RT) proximal (RTpi-MI) and distal intestine (RTdi-MI) compared them with the previously established continuous line RTgutGC. Intestinal stem cells, differentiating differentiated epithelial connective cells were found in all lines. The formed a polarized barrier, which was not permeable to large molecules absorbed proline glucose. High seeding density induced their differentiation into more mature phenotypes, as indicated by downregulation...
The potential antiviral effects of indole-3-carbinol (I3C), a phytochemical found in Cruciferous vegetables, were investigated. Fibroblasts and epithelial cells co-cultured on Alvetex® scaffolds, to obtain ad hoc 3D vitro platforms able mimic the trachea intestinal mucosae, which represent primary structures involved coronavirus pathogenesis. two barriers generated treated with various concentrations I3C for different incubation periods. A protective effect both models was demonstrated....
Abstract Understanding essential signaling network requirements and making appropriate adjustments in culture conditions are crucial if porcine pluripotent stem cells (PSC) to achieve their full potential. Here, we first used two protein factors (LIF FGF2) kinase inhibitor combinations attempts convert primed type lentiviral-reprogrammed induced PSC (Lv-piPSC) into naïve-like state developed a medium called FL6i. In addition FGF2 LIF, this contained inhibitors of MAPK14, MAPK8, TGFB1,...
Despite progress in reducing antimicrobial use the veterinary field, it is crucial to find alternatives preserve effectiveness and limit resistance. In pig farming, pathogenic strains of
We previously showed that, according to the frequency and distribution of specific cell types, rainbow trout (RT) intestinal mucosa can be divided in two regions that form a complex nonlinear three-dimensional (3D) pattern have different renewal rate. This work had aims. First, we investigated whether unusual populations reflects similar functional activities. To this end, determined protein expression three well-defined enterocytes markers: peptide transporter 1 (PepT1),...