A5106225935- Iron Metabolism and Disorders
- Erythropoietin and Anemia Treatment
- Dialysis and Renal Disease Management
- Hemoglobinopathies and Related Disorders
- Body Composition Measurement Techniques
- Chronic Kidney Disease and Diabetes
- Heme Oxygenase-1 and Carbon Monoxide
- Apelin-related biomedical research
- Pharmacological Effects and Toxicity Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism and Genetic Disorders
- Central Venous Catheters and Hemodialysis
- Electrolyte and hormonal disorders
- Paraoxonase enzyme and polymorphisms
- Blood transfusion and management
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Genomics and Rare Diseases
- Magnesium in Health and Disease
- Medication Adherence and Compliance
- Autoimmune Bullous Skin Diseases
- Thermoregulation and physiological responses
- Redox biology and oxidative stress
- Connexins and lens biology
- Nosocomial Infections in ICU
- Renal Transplantation Outcomes and Treatments
Institute of Cancer Research
2021
St George’s University Hospitals NHS Foundation Trust
2021
Rede de Química e Tecnologia
2012-2019
Universidade do Porto
2012-2019
NephroCare
2016
End-stage renal disease (ESRD) patients under hemodialysis (HD) have high mortality rate. Inflammation, dyslipidemia, disturbances in erythropoiesis, iron metabolism, endothelial function, and nutritional status been reported these patients. Our aim was to identify any significant association of death with disturbances, by performing a two-year follow-up study. A large set data obtained from 189 HD (55.0% male; 66.4 ± 13.9 years old), including hematological data, lipid profile, nutritional,...
Objective . To evaluate the effect of gender and menopause in cardiovascular risk (CVR) a healthy population based on both classical nontraditional markers. Methods 56 men 68 women (48 pre- 20 postmenopause) were enrolled study. The following markers analyzed: blood pressure (BP), body mass index (BMI), waist circumference (WC), glucose, total cholesterol (total-c), triglycerides (TGs), low-density lipoprotein (LDL-c), oxidized-LDL (Ox-LDL), HDL-c subpopulations, paraoxonase-1 activity,...
Anemia is a common complication of chronic kidney disease (CKD) that develops early and its severity increases as renal function declines. It mainly due to reduced production erythropoietin (EPO) by the kidneys; however, there are evidences iron metabolism disturbances increase CKD progresses. Our aim was study mechanisms underlying development anemia CKD, well damage, in remnant rat model induced 5/6 nephrectomy. This presented sustained degree dysfunction, with mild advanced glomerular...
Abstract Chronic kidney disease ( CKD ) has been associated with an abnormal lipid profile. Our aim was to study the interplay between oxidized low‐density lipoprotein ox‐LDL ), adiponectin, and blood lipids lipoproteins in Portuguese patients under hemodialysis HD ); influence of pentanucleotide repeat polymorphism apolipoprotein(a) apo [a] gene upon lipoprotein(a) Lp[a] levels these patients. We studied 187 25 healthy individuals. adiponectin were measured using enzyme‐linked immunoassays....
<b><i>Background/Aims:</i></b> Cardiovascular diseases are the major cause of morbidity and mortality in hemodialysis (HD) patients. These patients present reduced paraoxonase 1 (PON1) activity that depends on genetic non-genetic factors; however, how these factors influence PON1 HD is poorly clarified. Our aim was to evaluate two polymorphisms <b><i>Methods:</i></b> We evaluated 183 under recombinant human erythropoietin (rhEPO) treatment 22...
Our aim was to contribute a better understanding of the pathophysiology anemia in elderly, by studying how aging affects renal function, iron metabolism, erythropoiesis and inflammatory response, using an experimental animal model. The study performed male Wistar, group young rats with 2 months age old one 18 age. Old presented significant higher urea, creatinine, interferon (INF)-gamma, ferritin soluble transferrin receptor serum levels, as well increased counts reticulocytes RDW. In...
We evaluated the potential cardiovascular risk protection of bilirubin in hemodialysis (HD) patients. An enlarged set studies were 191 HD patients, including hematological study, lipid profile, iron metabolism, nutritional, inflammatory markers, and dialysis adequacy. The TA duplication screening UDP-glucuronosyltransferase 1 A1 ( UGT1A1 ) promoter region was also performed. genotype frequencies patients 49.2%, 42.4%, 8.4% for 6/6, 6/7, 7/7 genotypes, respectively. Although no difference...
The aim of this work was to contribute a better understanding the relationship between resistance recombinant human erythropoietin (rhEPO) therapy and body mass index (BMI) in hemodialysis (HD) patients. We evaluated 191 HD patients 25 healthy individuals. Complete blood count, reticulocyte circulating levels ferritin, transferrin, iron, soluble transferrin receptor (sTfR), saturation, hepcidin, C-reactive protein (CRP), interleukin 6 (IL-6), albumin, adiponectin were measured all controls....
This study aimed to elucidate the mechanisms explaining persistence of anemia and resistance recombinant human erythropoietin (rHuEPO) therapy in a rat model chronic kidney disease (CKD)-associated with formation anti-rHuEPO antibodies. The remnant CKD induced by 5/6 nephrectomy was used test long-term (nine weeks) high dose rHuEPO (200 UI/kg bw/week) treatment. Hematological biochemical parameters were evaluated as well serum tissue (kidney, liver and/or duodenum) protein gene expression...
Clinical studies showed that high doses of recombinant human erythropoietin (rHuEPO) used to correct anaemia in chronic kidney disease (CKD) hyporesponsive patients may lead deleterious effects. The aim this study was analyze the effects rHuEPO usually CKD-anaemia (100, 200 IU/kg body weight (BW) per week) and higher treatment (400, 600 BW week), focusing on renal damage, hypoxia, inflammation fibrosis. Male Wistar rats with failure (CRF) induced by 5/6 nephrectomy were treated or vehicle,...
Abstract Hepcidin is the major central regulator of iron metabolism, controlling absorption and mobilization. Considering its interaction with several factors that are altered in chronic kidney disease (CKD), particularly hyporesponsive CKD patients under therapy high recombinant human erythropoietin (rHuEPO) doses, it was aimed to study impact increasing rHuEPO doses on regulation iron‐hepcidin metabolism. The blood, cellular, tissue studies, using remnant rat model induced by 5/6...
Erythroid hypoplasia (EH) is a rare complication associated with recombinant human erythropoietin (rHuEPO) therapies, due to development of anti-rHuEPO antibodies; however, the underlying mechanisms remain poorly clarified. Our aim was manage rat model antibody-mediated EH induced by rHuEPO and study impact on iron metabolism erythropoiesis. Wistar rats treated during 9 weeks high dose (200 IU) developed EH, as shown anemia, reduced erythroblasts, reticulocytopenia, plasmatic antibodies....
Toll-like receptors (TLRs) play a key role in the response of innate and adaptive immune system to microbial endogenous ligands. Inflammation is common feature end-stage renal disease (ESRD) patients; however, mechanisms/factors triggering inflammatory process are still poorly clarified. Our aim was analyze impact c.-1486T>C c.896A>G polymorphisms TLR9 TLR4 genes, respectively, on ESRD patients. Clinical laboratory evaluation carried out 184 Polymerase chain reaction followed by...
Purpose The aim of this work was to assess the effect statin therapy on inflammatory and fibrinolytic/endothelial (dys)function markers in end-stage renal disease (ESRD) patients under hemodialysis (HD), according type vascular access. Methods This transversal study includes 191 ESRD regular HD, divided into four groups access therapy: 87 with arteriovenous fistula (AVF) no statins (AVF-NS), 61 AVF (AVF-S), 27 central venous dialysis catheter (CVC) (CVC-NS) 16 CVC (CVC-S). basic lipid...