Pseudomonas putida metabolizes Phe and Tyr through a peripheral pathway involving hydroxylation of to (PhhAB), conversion into 4-hydroxyphenylpyruvate (TyrB), formation homogentisate (Hpd) as the central intermediate. Homogentisate is then catabolized by catabolic that involves three enzymes, dioxygenase (HmgA), fumarylacetoacetate hydrolase (HmgB), maleylacetoacetate isomerase (HmgC), finally yielding fumarate acetoacetate. Whereas phh, tyr, hpd genes are not linked in P. genome, hmgABC...

Summary Background Anaphylaxis against Anucardiuccae nuts is uncommon and the allergens involved still poorly characterized. For this reason two patients with allergy towards pistachio nut (a member of Anacardiaceae family) have been studied. Objective Identification immunoallergens present in analysis crossreactive antigens other members same plant family, specifically cashew mango. Methads Presence specific IgE for mango seed pulp was determined by skin tests radioallergosorbent assay...

Summary Background Seed proteins have been found to cause hypersensitivity by ingestion or inhalation. Rapeseed fiour was responsible for allergic symptoms in a patient, who develops into allergy mustard spice. Objective To determine the presence of allergenic rapeseed fiour, and analyse structure main component its crossreactivity with allergeti. Methods SDS‐PAGE (sodium dodecyl sulfate‐polyacrylamide gel electrophoresis) subsequent immunoblotting serum from patient were performed detect...

Phenylacetic acid (PA) degradation in bacteria involves an aerobic hybrid pathway encoded by the paa gene cluster. It is shown here that succinyl-CoA one of final products this Pseudomonas putida and Escherichia coli. Moreover, vivo vitro studies revealed paaE encodes beta-ketoadipyl-CoA thiolase catalyses last step PA catabolic pathway, i.e. thiolytic cleavage to acetyl-CoA. Succinyl-CoA suggested as a common product pathways devoted catabolism aromatic compounds.

Cytochrome P450 3A4 (CYP3A4) is the dominant xenobiotic metabolizing CYP. Despite great interest in CYP enzymology, two vitro aspects of CYP3A4 catalysis are still not well understood, namely, sequential metabolism and allosteric activation. We have therefore investigated such a system which both phenomena present. Here we report that Nile Red (NR) accelerated by heterotropic effector α-naphthoflavone (ANF). ANF increases rates formation for NR metabolites M1 M2 also perturbs metabolite...

The paa genes for phenylacetic acid (PA) catabolism encode the best characterized aerobic hybrid route involved in bacterial degradation of aromatic compounds. Here, we demonstrate that divergent paaZ and paaA-K catabolic operons Escherichia coli are regulated by two genes, paaXY, form a distinct transcriptional unit driven Px promoter. In vivo vitro approaches using purified PaaX regulatory protein revealed this regulator is able to bind inhibit activity phenylacetyl-coenzyme A (PA-CoA)...

Introduction: Dietary habits and healthy lifestyles are crucial factors impacting cardiometabolic health quality of life. Precision nutrition has emerged as a valuable tool to monitor the multiple participating in metabolic wellbeing examine possible interactions between diet health. One such approach involves use dimensional reduction methods, which aim classify subjects into distinct nutritional subgroups or dietotypes based on differential dietary intakes outcomes. Methods Results: A...

This paper presents an algorithm for CT saturation detection based on the measurement of power system source impedance seen at relay location. The estimates this using a short-data window impedance-estimation and samples taken from current transformer (CT) secondary busbar voltage. Following changes location, is able to detect send blocking order fast tripping differential relay.

To evaluate the use of contrast-enhanced ultrasound (CEUS) for diagnosis cortical necrosis in renal allografts.We reviewed medical records and imaging studies five patients who underwent emergency transplantectomy a histological period between May 2009 2011. US examinations included initially B-mode color Doppler then with low mechanical index after injection 2.4 ml second generation echo-signal enhancer. Renal transplant vascularization was evaluated during 4 minutes including arterial,...

Cytochrome P450 3A4 (CYP3A4) plays a critical role in the metabolism of many drugs. CYP3A4 exhibits extraordinary substrate promiscuity and unusual allosteric kinetics. In addition, CYPs catalyze sequential oxidations on single substrate, but most cases, mechanistic details these processes are not well-established. As result, vivo clearance drugs their metabolites is difficult to predict basis complex vitro kinetics, new probes required understand behaviors. The near-IR fluorescent probe...

RepA-WH1 is a disease-unrelated protein that recapitulates in bacteria key aspects of human amyloid proteinopathies: i) It undergoes ligand-promoted amyloidogenesis vitro; ii) its aggregates are able to seed/template amyloidosis on soluble molecules; iii) conformation modulated by Hsp70 chaperones vivo, generating transmissible strains; and iv) causes proliferative senescence. Membrane disruption amyloidogenic oligomers has been found for most proteins causing neurodegenerative diseases....

This paper presents an algorithm for current transformer (CT) saturation detection based on the measurement of power system source impedance seen at relay location. The estimates this using a short-data window impedance-estimation and samples taken from CT secondary bus-bar voltage. Following changes location, is able to detect send blocking order fast tripping differential relay.

We show here that the paaABCDE genes of paa cluster responsible for phenylacetate degradation in Escherichia coli W encode a five-component oxygenase hydroxylates phenylacetyl-coenzyme A (CoA), first intermediate pathway. The primary structure subunits bacterial phenylacetyl-CoA oxygenases revealed these enzymes constitute prototype new and distinct group large diiron multicomponent family.

Abstract Understanding protein amyloidogenesis is an important topic in science, fueled by the role of amyloid aggregates, especially oligomers, etiology a number devastating human degenerative diseases. However, mechanisms that determine formation oligomers remain elusive due to high complexity process. For instance, gold nanoparticles promote or inhibit fibrillation. We have functionalized nanorods with metal‐chelating group selectively immobilize soluble RepA‐WH1, model synthetic...

Under conditions relevant to the manufacturing of insulin (e.g., pH 3, room temperature), biosynthetic human (BHI), and Lispro (Lispro) require a nucleation step initiate aggregation. However, upon seeding with preformed aggregates, both insulins rapidly aggregate into nonnative fibrils. Far ultraviolet circular dichroism (far-UV CD) second derivative Fourier transform infrared (2D-FTIR) spectroscopic analyses show that fibrillation process involves change in protein secondary structure from...

ABSTRACT The P styA promoter of Pseudomonas sp. strain Y2 controls expression the styABCD genes, which are required for conversion styrene to phenylacetate, is further catabolized by products two paa gene clusters. Two PaaX repressor proteins (PaaX1 and PaaX2) regulate transcription clusters this strain. In silico analysis region revealed a sequence located just within that similar reported binding sites Escherichia coli proposed genes species. Here we show protein extracts from some strains...