A5019467735- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Genomics and Phylogenetic Studies
- Analytical Chemistry and Chromatography
- Plant biochemistry and biosynthesis
- Cancer, Lipids, and Metabolism
- Plant Pathogens and Fungal Diseases
- Ginseng Biological Effects and Applications
- Glycosylation and Glycoproteins Research
- Plant and Fungal Species Descriptions
- Lipid metabolism and biosynthesis
- Electrocatalysts for Energy Conversion
- Genetic diversity and population structure
- Plant Gene Expression Analysis
- Plasma Applications and Diagnostics
- Traditional Chinese Medicine Analysis
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Protist diversity and phylogeny
- Biochemical and Structural Characterization
- Agriculture, Soil, Plant Science
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- Fungal Biology and Applications
- Amphibian and Reptile Biology
China Academy of Chinese Medical Sciences
2021-2025
Liaocheng University
2024
Beijing Anzhen Hospital
2024
Capital Medical University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2013-2023
Chengdu University of Traditional Chinese Medicine
2020-2023
China Pharmaceutical University
2019-2021
Henan University of Science and Technology
2018-2020
Liaocheng People's Hospital
2018
Taizhou Central Hospital
2016-2017
Six different types of cancer (i.e., breast, lung, colorectal, esophageal, gastric and thyroid cancer) have high rates incidence or mortality worldwide. It has been shown that activation de novo lipogenesis is an early common event in the microenvironment. In this study, we performed lipid imaging profiling for 134 tissue samples from six using matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry, with 2,5-dihydroxybenzoic acid...
RATIONALE Colorectal cancer (CRC) has attracted increasing attention due to its common occurrence and worldwide distribution. METHODS Direct‐infusion positive negative ion electrospray ionization Fourier transform cyclotron resonance mass spectrometry (DI‐ESI(±)‐FTICR MS) was applied analyze the serum metabolites from 52 CRC patients healthy controls. Metabolites whose inter‐group intensities were determined be statistically significant by univariate multivariate statistical analyses further...
Abstract Lipid composition in cell membrane is closely associated with characteristics. Here, matrix-assisted laser desorption/ionization- Fourier transform ion cyclotron resonance mass spectrometry was employed to situ determine components of human mammary epithelial cells (MCF-10 A) and six different breast cancer lines ( i.e. , BT-20, MCF-7, SK-BR-3, MDA-MB-231, MDA-MB-157 MDA-MB-361) without any lipid extraction separation. Partial least-square discriminant analysis indicated that...
Paeonia ostii, a common oil-tree peony, is important ornamentally and medicinally. However, there are few studies on the chloroplast genome of ostii. We sequenced analyzed complete P. The size ostii 152,153 bp, including large single-copy region (85,373 bp), small (17,054 pair inverted repeats regions (24,863 bp). encodes 111 genes, 77 protein-coding four ribosomal RNA 30 transfer genes. contains forward (22), palindromic (28), tandem (24). presence rich simple-sequence repeat loci in...
Herbgenomics is an emerging field of traditional Chinese medicine (TCM) research and development. By combining TCM with genomics, herbgenomics can help to establish the scientific validity bring it into wider usage within medicine. Salvia Linn. ( S . Linn.) a large genus Labiatae that includes important medicinal plants. In this study, complete chloroplast (cp) genomes two spp.—namely, S. przewalskii bulleyana , which are used as surrogate for miltiorrhiza —were sequenced compared those...
We sequenced and analyzed the complete chloroplast genome of Aster tataricus (family Asteraceae), a Chinese herb used medicinally to relieve coughs reduce sputum. The A. was 152,992 bp in size, harbored pair inverted repeat regions (IRa IRb, each 24,850 bp) divided into large single-copy (LSC, 84,698 small (SSC, 18,250 region. Our annotation revealed that contained 115 genes, including 81 protein-coding 4 ribosomal RNA 30 transfer genes. In addition, 70 simple sequence repeats (SSRs) were...
Small molecule metabolites (SMMs, typically <500 Da) are important cellular constituents closely associated with tumor development and progression. However, in situ label-free detection of tissue SMMs has been limited due to interference from matrix and/or low sensitivity. Herein, we develop an electric field-assisted scanning-spraying (EFASS) coating system deposit on crystal sizes <10 μm, followed by matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) negative...
Polygonum is a generalized genus of the Polygonaceae family that includes various herbaceous plants. In order to provide aid in understanding evolutionary and phylogenetic relationship at chloroplast (cp) genome-scale level, we sequenced annotated complete genomes four species using next-generation sequencing technology CpGAVAS. Then, repeat sequences, IR contractions, expansion transformation sites were studied, tree was built Polygonum. The results indicated genome construction also...
Brain lipid homoeostasis is critical during recovery process after traumatic brain injury (TBI). In this study, we integrated liquid extraction and electrosonic spray ionization technology to develop an device coupled with a Fourier transform ion cyclotron resonance mass spectrometer for imaging of docosahexaenoic acid (DHA)-containing lipids on rat tissues. The images the tissue sections from normal rats TBI at acute phase (0 1 day) chronic (3, 5, 7 days) were obtained. results indicate...
The development of on-tissue chemical derivatization methods for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) small endogenous metabolites in tissues has attracted great attention their advantages improving detection sensitivity and ionization efficiency poorly ionized low abundant metabolites. Herein, a laser-assisted tissue transfer (LATT) technique was developed to enhance molecules. Using focused blue laser, thin-layer film (∼1 μm) transferred an...
Safflower (Carthamus tinctorius) is widely cultivated around the world for its seeds and flowers. The presence of linoleic acid (LA) in hydroxysafflor yellow A (HSYA) flowers are crucial traits that enable safflower to be used industrial medicinal purposes. Understanding genetic control these essential optimizing quality breeding. To further this research, we present a chromosome-scale assembly genome variety 'Chuanhonghua 1', which was achieved using an integrated strategy combining...
The interactions of IgG Fc region with receptors are optimized by the tailoring a single-conserved N-linked glycosylation site at Asn-297. Our previous study has demonstrated that age-related Fc-glycosylation change is featured sex specificity and potential for disease discrimination. Here, we conducted Fourier transform ion cyclotron resonance MS-based profiling involving 410 control individuals 259 lung cancer (LC) patients. As compared to healthy controls, marked increase in IgG1...
Abstract The discrimination and detection of phosphate anions have attracted extensive attention due to their important roles in various biological processes. Compared with sensors detect one individual at a time, sensor arrays are able discriminate multiple phosphates simultaneously. In this study, we developed rare earth ions enhanced AuNCs‐based array achieve facile rapid identification (PPi, ADP ATP). (i. e., Ce 3+ , Gd Tm Yb ) can significantly enhance the fluorescence AuNCs through...
Tropane alkaloids (TAs), which are anticholinergic agents, an essential class of natural compounds. There is a growing demand for these compounds with anesthetic, analgesic, and spasmolytic effects. Anisodus acutangulus TA-producing plant (family Solanaceae) that was used as anesthetic in ancient China. In this study, high-quality chromosome-scale A. genome assembled, contig N50 up to 7.4 Mb. acutangulus, recent whole-genome duplication event occurred after the divergence from other...
<title>Abstract</title> <italic>Nothapodytes nimmoniana</italic> is known to produce the highest content of anticancer compound camptothecin (CPT) in plant kingdom. We present chromosome-level allotetraploid genome <italic>N. nimmoniana</italic>, marking first sequence from order Icacinales. This 5-Gb encodes 92,630 genes, with subgenome B exhibiting dominant gene expression. Through mining, we identified and characterized four key enzymes involved CPT biosynthesis, revealing that shares a...