A5103117429- Eicosanoids and Hypertension Pharmacology
- Inflammatory mediators and NSAID effects
- Cancer, Hypoxia, and Metabolism
- Alcohol Consumption and Health Effects
- Infant Nutrition and Health
- Nitric Oxide and Endothelin Effects
- Amino Acid Enzymes and Metabolism
- Pesticide Residue Analysis and Safety
- Metabolism and Genetic Disorders
- Liver Disease Diagnosis and Treatment
- Peroxisome Proliferator-Activated Receptors
- Biochemical effects in animals
- Hormonal Regulation and Hypertension
- Animal Ecology and Behavior Studies
- Liver Disease and Transplantation
- Pharmacogenetics and Drug Metabolism
- Antimicrobial Peptides and Activities
- Gut microbiota and health
- Analytical chemistry methods development
Carleton University
2019
University of California, Davis
2015-2019
UC Davis Comprehensive Cancer Center
2017-2019
Significance This study furthers our understanding of epoxyeicosatrienoic acid metabolism by cyclooxygenase (COX) enzymes as a physiologically relevant metabolic pathway, producing signaling molecules that are angiogenic. It explains, in part, why inhibiting the soluble epoxide hydrolase (sEH) some systems is angiogenic whereas combining sEH inhibition with COX dramatically antiangiogenic, which turn may suppress tumor growth.
Since polycystic kidney disease (PKD) was first noted over 30 years ago to have neoplastic parallels, there has been a resurgent interest in elucidating neoplasia-relevant pathways PKD. Taking nontargeted metabolomics approach the B6(Cg)-Cys1(cpk/)J (cpk) mouse model of recessive PKD, we now characterized metabolic reprogramming these tissues, leading glutamine-dependent TCA cycle shunt toward total 2-hydroxyglutarate (2-HG) production cpk compared with B6 wild-type tissue. After...
Epoxyeicosatrienoic acids (EETs) are formed from the metabolism of arachidonic acid by cytochrome P450s. EETs promote angiogenesis linked to tumor growth in various cancer models that is attenuated vivo cyclooxygenase 2 (COX-2) inhibitors. This study further defines a role for COX-2 mediating endothelial EET promoting angiogenesis. Using human aortic cells (HAECs), we quantified 8,9-EET-induced tube formation and cell migration as indicators angiogenic potential presence absence inducer...
Tetramethylenedisulfotetramine (TETS, tetramine) is a formerly used and highly neurotoxic rodenticide. Its lethality, recent history of intentional use for mass poisoning, the absence known antidote raise public health concerns. Therefore, rapid, high throughput, sensitive methods detection quantification TETS are critical. Instrumental analysis method such as GC/MS but not rapid or throughput. an immunoassay selective to was developed. The assay shows IC50 4.5 ± 1.2 ng/mL, with limit 0.2...
The cyclooxygenase-2 (COX-2) selective inhibitor celecoxib is widely used in the treatment of pain and inflammation. Celecoxib has been explored as a possible liver fibrosis with contradictory results, depending on model. present study reports effect 5-week carbon tetrachloride (CCl<sub>4</sub>)-induced mouse alone combination inhibitors enzyme-soluble epoxide hydrolase (sEH), well dual that targets both COX-2 sEH, were administered via osmotic minipump to mice receiving intraperitoneal...
COX metabolites of 8,9-EET, previously observed as potent mitogenic lipid mediators, were synthesized for the first time by using two synthetic approaches.
Omega-6 and omega-3 polyunsaturated fatty acids are precursors to chemical mediators of angiogenesis, important in many human diseases including cancer. Respectively, epoxyeicosatetraenoic (EETs) lead pro-angiogenic effects, whereas epoxydocosapentaenoic (EDP) anti-angiogenic. Epoxide hydrolase catalyzes the breakdown these endogenously active intermediates, has been identified as a promising target for angiogenic modulatory therapy. However, vivo effects soluble epoxide inhibitor (sEHI)...