A5028919490- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Neuropeptides and Animal Physiology
- HIV Research and Treatment
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Pharmacological Receptor Mechanisms and Effects
- RNA Interference and Gene Delivery
- Microtubule and mitosis dynamics
- Protein Kinase Regulation and GTPase Signaling
- Calcium signaling and nucleotide metabolism
- Signaling Pathways in Disease
- Neurogenesis and neuroplasticity mechanisms
- Cancer, Stress, Anesthesia, and Immune Response
- Pituitary Gland Disorders and Treatments
- Bone Tissue Engineering Materials
- Ion Transport and Channel Regulation
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Hippo pathway signaling and YAP/TAZ
- Hepatitis C virus research
- RNA modifications and cancer
- Pharmacological Effects and Assays
- Electrospun Nanofibers in Biomedical Applications
- Polymer Surface Interaction Studies
Centre National de la Recherche Scientifique
2006-2024
Institut des Neurosciences Cellulaires et Intégratives
2009-2023
Université de Strasbourg
2009-2023
Institut de génétique et de biologie moléculaire et cellulaire
2004-2013
Inserm
2002-2006
Hungarian Academy of Sciences
1997-1998
HUN-REN Szegedi Biológiai Kutatóközpont
1998
Institute of Biochemistry
1998
Laboratoire de Psychologie et NeuroCognition
1998
The combination of fluorescent genetically encoded proteins with mouse engineering provides a fascinating means to study dynamic biological processes in mammals. At present, green protein (GFP) mice were mainly developed gene expression patterns or cell morphology and migration. Here we used enhanced GFP (EGFP) achieve functional imaging G protein-coupled receptor (GPCR) vivo. We created where the delta-opioid (DOR) is replaced by an active DOR-EGFP fusion. Confocal revealed detailed...
Abstract The aim of this study was to develop new biocompatible coatings for bone implants by the alternating deposition oppositely charged polyelectrolytes. Polyelectrolyte films were built up with different terminating layers on which SaOS‐2 osteoblast‐like cells and human periodontal ligament (PDL) grown. layer made one following polyelectrolytes: poly(ethylene imine) (PEI), poly(sodium 4‐styrenesulfonate) (PSS), poly(allylamine hydrochloride) (PAH), poly( L ‐glutamic acid) (PGA), or...
GPCRs regulate a remarkable diversity of biological functions, and are thus often targeted for drug therapies. Stimulation GPCR by an extracellular ligand triggers receptor signaling via G proteins, this process is highly regulated. Receptor activation typically accompanied desensitization signaling, complex feedback regulatory which internalization postulated as key event. The in vivo significance poorly understood. In fact, the majority studies have been performed transfected cell systems,...
Membrane fusion is a process that intimately involves both proteins and lipids. Although the SNARE proteins, which ultimately overcome energy barrier for fusion, have been extensively studied, regulation of itself, determined by specific membrane lipids, has largely overlooked. Our findings reveal novel function in reducing directly binding sequestering fusogenic lipids to sites fusion. We demonstrate interaction between Syntaxin1A lipid phosphatidic acid, addition multiple...
Apical neural progenitors are polarized cells for which the apical membrane is site of cell-cell and cell-extracellular matrix adhesion events that essential maintaining integrity developing neuroepithelium. important several aspects nervous system development, including morphogenesis neurogenesis, yet mechanisms underlying its regulation remain poorly understood. Here, we show ephrin B1, a cell surface protein engages in signaling upon binding cognate Eph receptors, controls normal cortex....
An important role for specific lipids in membrane fusion has recently emerged, but regulation of their biosynthesis remains poorly understood. Among fusogenic lipids, phosphatidic acid and phosphoinositol 4,5-bisphosphate (PIP(2)) have been proposed to act at various steps neurotransmitter hormone exocytosis. Using real time FRET (fluorescence resonance energy transfer) measurements, we show here that the GTPase ARF6, potentially involved synthesis these is activated exocytotic sites PC12...
Most HIV-1 Tat is unconventionally secreted by infected cells following interaction with phosphatidylinositol (4,5) bisphosphate (PI(4,5)P2) at the plasma membrane. Extracellular endocytosed uninfected before escaping from endosomes to reach cytosol and bind PI(4,5)P2. It not clear whether how incoming concentrates in cells. Here we show that, cells, S-acyl transferase DHHC-20 together prolylisomerases cyclophilin A (CypA) FKBP12 palmitoylate on Cys31 thereby increasing affinity for In CypA...
Summary HIV-1 transcriptional activator (Tat) enables viral transcription and is also actively released by infected cells. Extracellular Tat can enter uninfected cells affect some cellular functions. Here, we examine the effects of protein on secretory activity neuroendocrine When added to culture medium chromaffin PC12 cells, was internalized strongly impaired exocytosis as measured carbon fiber amperometry growth hormone release assay. Expression mutants that do not bind...
Abstract Among small GTPases from the Rho family, Cdc42, Rac, and are well known to mediate a large variety of cellular processes linked with cancer biology through their ability cycle between an inactive (GDP-bound) active (GTP-bound) state. Guanine nucleotide exchange factors (GEFs) stimulate GDP for GTP generate activated form, whereas GTPase-activating proteins (GAPs) catalyze hydrolysis, leading inactivated form. Modulation GTPase activity following altered expression Rho-GEFs and/or...
The delta opioid receptor modulates nociceptive and emotional behaviors. This has been shown to exhibit measurable spontaneous activity. Progress in understanding the biological relevance of this activity slow, partly due limited characterization compounds with intrinsic negative Here, we have used constitutively active mutant (CAM) receptors two different functional assays, guanosine 5′-<i>O</i>-(3-thio)triphosphate binding a reporter gene assay, test potential inverse agonism 15 compounds,...
Phospholipids (PLs) are asymmetrically distributed at the plasma membrane. This asymmetric lipid distribution is transiently altered during calcium-regulated exocytosis, but impact of this transient remodeling on presynaptic function currently unknown. As phospholipid scramblase 1 (PLSCR1) randomizes PL between two leaflets membrane in response to calcium activation, we set out determine its role neurotransmission. We report here that PLSCR1 expressed cerebellar granule cells (GrCs) and...
Numerous severe neurodegenerative and neuromuscular disorders, characterized biochemically by strong perturbations in energy metabolism, are correlated with single point mutations mitochondrial genes coding for transfer RNAs. Initial comparative proteomics performed on wild-type Myoclonic Epilepsy Ragged Red Fibers (MERRF) mitochondria from sibling human cybrid cell lines revealed the potential of this approach. Here a quantitative analysis several hundred silver-stained spots separated...
In the central nervous system, opioid receptors are found in neurons and also glial cells. To gain more information on their presence possibly function, we investigated expression of mu-opioid (MOR) during oligodendroglial cell development two culture systems. these models, first days, cells O-2A bipotential progenitor (also called OPCs; oligodendrocyte precursor cells), then they differentiate into oligodendrocytes, which mature. cells, derived from newborn rat brain hemispheres, grown...
Human immunodeficiency virus (HIV)-infected cells actively release the transcriptional activator (Tat) viral protein that is required for efficient HIV gene transcription. We recently reported extracellular Tat able to enter uninfected neurosecretory cells. Internalized escapes endosomes reach cytosol and then recruited plasma membrane by phosphatidylinositol 4,5-bisphophate (PtdIns(4,5)P 2). strongly impairs exocytosis from chromaffin PC12 perturbs synaptic vesicle exo-endocytosis cycle...
ABSTRACT Rho GTPases play a key role in various membrane trafficking processes. RhoU is an atypical small GTPase related to Rac/Cdc42, which possesses unique N- and C-terminal domains that regulate its function subcellular localization. localizes at the plasma membrane, on endosomes cell adhesion structures where it governs signaling, differentiation migration. However, despite endomembrane localization, vesicular has been unexplored. Here, we identified intersectins (ITSNs) as new binding...
Abstract Structural phospholipids are asymmetrically distributed at the plasma membrane, with phosphatidylethanolamine and phosphatidylserine (PS) virtually absent from outer leaflet. This asymmetric lipid distribution is transiently altered during specific biological processes including calcium-regulated exocytosis. However, impact of this transient remodeling membrane asymmetry on presynaptic function remains unknown. PhosphoLipid SCRamblase 1 (PLSCR1), a protein that randomizes...
et à la diffusion de documents scientifiques niveau recherche, publiés ou non, émanant des établissements d'enseignement recherche français étrangers, laboratoires publics privés.
During the last two decades, it has become evident that cellular lipids not only form bilayers to separate compartments, but also participate in vesicular trafficking and signal transduction. Phosphoinositides have received major attention at this respect, other phospholipids such as phosphatidic acid (PA) are quickly emerging signaling molecules. The spatiotemporal distribution of PA appears tightly regulated by localized synthesis quick degradation. Although been reported plays key roles...