A5066018585- Sodium Intake and Health
- Atherosclerosis and Cardiovascular Diseases
- Hormonal Regulation and Hypertension
- Blood Pressure and Hypertension Studies
- Cancer Immunotherapy and Biomarkers
- Aortic aneurysm repair treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Immune Cell Function and Interaction
- Fungal and yeast genetics research
- Cytokine Signaling Pathways and Interactions
- Genetic Associations and Epidemiology
- Endoplasmic Reticulum Stress and Disease
- Tracheal and airway disorders
- CAR-T cell therapy research
- Psoriasis: Treatment and Pathogenesis
- Birth, Development, and Health
- Myasthenia Gravis and Thymoma
- Cell Adhesion Molecules Research
- Aortic Disease and Treatment Approaches
- Artificial Intelligence in Healthcare and Education
- Epigenetics and DNA Methylation
- Cancer, Stress, Anesthesia, and Immune Response
- Redox biology and oxidative stress
- SARS-CoV-2 detection and testing
- Biofuel production and bioconversion
Vanderbilt University Medical Center
2017-2025
Vanderbilt University
2018-2025
TESLA (Czechia)
2024
Columbia University
2023-2024
Morgan Stanley Children's Hospital
2023-2024
Vanderbilt Health
2023
Biology of Infection
2023
Zero to Three
2020
Cardiovascular Research Center
2019
St. Matthew's University
2018-2019
Immune checkpoint inhibitors have improved clinical outcomes associated with numerous cancers, but high-grade, immune-related adverse events can occur, particularly combination immunotherapy. We report the cases of two patients melanoma in whom fatal myocarditis developed after treatment ipilimumab and nivolumab. In both patients, there was development myositis rhabdomyolysis, early progressive refractory cardiac electrical instability, a robust presence T-cell macrophage infiltrates....
Abstract Background: Natural language processing models such as ChatGPT can generate text-based content and are poised to become a major information source in medicine beyond. The accuracy completeness of for medical queries is not known. Methods: Thirty-three physicians across 17 specialties generated 284 questions that they subjectively classified easy, medium, or hard with either binary (yes/no) descriptive answers. then graded ChatGPT-generated answers these (6-point Likert scale; range...
Importance Natural language processing tools, such as ChatGPT (generative pretrained transformer, hereafter referred to chatbot), have the potential radically enhance accessibility of medical information for health professionals and patients. Assessing safety efficacy these tools in answering physician-generated questions is critical determining their suitability clinical settings, facilitating complex decision-making, optimizing care efficiency. Objective To assess accuracy...
Atherosclerosis is a vascular disease characterized by lipid deposition and inflammation within the arterial wall. Oxidized phospholipids (oxPLs), such as 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (oxPAPC) its constituents 1-palmytoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine (POVPC) 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine (PGPC) are concentrated atherosclerotic lesions known to be potent proinflammatory mediators. Phenotypic switching of smooth muscle cells...
Clinical complications of atherosclerosis arise primarily as a result luminal obstruction due to atherosclerotic plaque growth, with inadequate outward vessel remodeling and destabilization leading rupture. IL-1 is proinflammatory cytokine that promotes atherogenesis in animal models, but its role unclear. The studies presented herein show advanced plaques mice lacking both receptor type I apolipoprotein E (Il1r1–/–Apoe–/– mice) unexpectedly exhibited multiple features instability compared...
Smooth muscle cell (SMC) phenotypic modulation in atherosclerosis and response to PDGF vitro involves repression of differentiation marker genes increases SMC proliferation, migration, matrix synthesis. However, SMCs within atherosclerotic plaques can also express a number proinflammatory genes, cultured the inflammatory cytokine IL-1β represses gene expression induces expression. Studies herein tested hypothesis that modulates phenotype distinct state relative PDGF-DD. Genome-wide analysis...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of global pandemic disease-2019 (COVID-19). SARS-CoV-2 a zoonotic disease, but little known about variations in species susceptibility that could identify potential reservoir species, animal models, and risk to pets, wildlife, livestock. Certain such as domestic cats tigers, are susceptible infection, while other mice chickens not. Most including those close contact with humans, have unknown susceptibility. Hence,...
Evidence from nonhuman animal models demonstrates an important role for immune cells in hypertension, but cell changes human hypertension are less clear. Using mass cytometry, we demonstrate novel and selective reductions CCR10
Exposure of yeast cells to an increase in external osmolarity induces a temporary growth arrest. Recovery from this stress is mediated by the accumulation intracellular glycerol and transcription several response genes. Increased causes transient 1N 2N concomitant depletion S phase cells. Hypertonic triggers cell cycle delay G2 that appears distinct morphogenesis checkpoint, which operates early decrease CLB2 mRNA, phosphorylation Cdc28p, inhibition Clb2p-Cdc28p kinase activity, whereas Clb2...
Background: SH2B3 (SH2B adaptor protein 3) is an that negatively regulates cytokine signaling and cell proliferation. A common missense single nucleotide polymorphism in (rs3184504) results substitution of tryptophan (Trp) for arginine (Arg) at amino acid 262 a top association signal hypertension human genome-wide studies. Whether this variant causal hypertension, if so, the mechanism by which it impacts pathogenesis unknown. Methods: We used CRISPR-Cas9 technology to create mice homozygous...
T and B cells have been implicated in hypertension, but the mechanisms by which they produce a coordinated response is unknown. follicular helper (Tfh) that interleukin 21 (IL21) promote germinal center (GC) cell responses leading to immunoglobulin (Ig) production. Here we investigate role of IL21 Tfh hypertension. In angiotensin (Ang) II-induced production increased, Il21-/- mice develop blunted attenuated vascular end-organ damage, decreased 17A (IL17A) interferon gamma Tfh-like GC...
The potential hemocompatibility of radiofrequency glow discharge (RFGD) polymers made by copolymerization mixtures hexafluoropropene and ethylene (C(3)F(6)/C(2)H(4)) or acrylic acid 1,7-octadiene was investigated using in vitro assays for platelet adhesion catalyzed thrombin generation. Thrombin generation rate normalized to number used as a measurement activation (procoagulant activity). RFGD produced 1, 7-octadiene contained varying amounts carboxylic species determined electron...
We confirmed that, when immunized with a conventional complete Freund adjuvant (water in oil), Lewis rats were highly susceptible to arthritis, Fisher less susceptible, and Buffalo much susceptible. However, mycobacterial delipidated cells squalane (squalane-type adjuvant) produced severe arthritis almost 100% incidence even the rat strains except for rats. With regard an immune response, induced strong delayed hypersensitivity purified protein derivative (PPD) peptidoglycan (PG) all used,...
Monocytes play a critical role in hypertension. The purpose of our study was to use an unbiased approach determine whether hypertensive individuals on conventional therapy exhibit altered monocyte gene expression profile and perform validation studies selected genes identify novel therapeutic targets for hypertension.Next generation RNA sequencing identified differentially expressed small discovery cohort normotensive individuals. Several these were further investigated association with...
Natriuretic peptide signaling is important in the regulation of blood pressure as well growth multiple cell types. To examine role natriuretic atherosclerosis, we crossbred mice that lack receptor A (NPRA; Npr1-/-) with atherosclerosis-prone apolipoprotein E (apoE; Apoe-/-).Doubly deficient Npr1-/-Apoe-/- have increased relative to Npr1+/+Apoe-/- (118+/-4 mm Hg compared 108+/-2 Hg, P<0.05) coincident a 64% greater atherosclerotic lesion size (P<0.005) and more advanced plaque morphology....
Aortic dissection (AD) is a life-threatening vascular disease with limited treatment strategies. Here, we show that loss of the GWAS-identified SH2B3 gene, encoding lymphocyte adaptor protein LNK, markedly increases susceptibility to acute AD and rupture in response angiotensin (Ang) II infusion. As early as day 3 following Ang infusion, prior development AD, Lnk-/- aortas display altered mechanical properties, increased elastin breaks, collagen thinning, enhanced neutrophil accumulation,...