[Role of SPP1 in acute kidney injury induced by renal ischemia-reperfusion in rats].

• DOI: 10.12122/j.issn.1673-4254.2023.11.16
• Publication Date: 2023-11-20
• Authors: Gang Yu; Xiaolin Wang; Jia Luo; Xiulan Su; Hong Tao; Zhuzhi Wen; Han Guan
• Keywords: Blood urea nitrogen; Renal ischemia; Acute tubular necrosis

To investigate the role of SPP1 gene in acute kidney injury induced by renal ischemia-reperfusion (IRI).Twelve Sprague-Dawley rats were randomly divided into sham group and IRI (n=6) subjected to operation ischemia for 30 min penal pedicle clamping using non-traumatic microvascular clamps, respectively.Serum creatinine blood urea nitrogen levels detected, PAS staining was used pathological examination kidneys two groups.The expressions SPP1, α-SMA caspase-3 detected immunohistochemistry immunofluorescent staining.In cultured tubular epithelial cells (HK-2 cells), Western blotting performed detect changes caspase-3, Kim-1 proteins following hypoxiareoxygenation (H/R) transfection with si-NC or si-SPP1;flow cytometry employed analyze apoptosis treated cells.Renal caused significant elevations serum (P<0.05) severe shedding necrosis rats, resulting also significantly up-regulated caspase-3(P<0.05).In HK-2 cells, H/R increased protein expression Kim-1(P<0.05), compared transfection, obviously reduced lowered rate exposure (P<0.05).SPP1 is IRI, down-regulation can inhibit H/R-induced cells.