[Sphingosine kinase-1 regulates migration and invasion of gastric cancer cells

• DOI: 10.12122/j.issn.1673-4254.2024.11.13
• Publication Date: 2024-11-20
• Authors: Qianlong Ling; Kai Ji; Jing Chen; Jun Guan; Rui Wang; Wanrong Man; Bing Zhu
• Keywords: Sphingosine kinase 1

To investigate the role of sphingosine kinase-1 (SPHK1) in regulating migration and invasion gastric cancer (GC) cells. TIMER2.0, GEPIA HPA databases were used to SPHK1 expression GC, its association with prognosis patients was analyzed using Kaplan-Meier Plotter database. In 40 clinical GC adjacent tissue samples, MKI67 expressions detected immunohistochemistry, Western blotting, RT-qPCR. Gene enrichment pathway analysis conducted explore biological functions SPHK1. HGC-27 MGC-803 cells, effects lentivirus-mediated knockdown or overexpression on cell key proteins nuclear factor-κB (NF-κB) signaling evaluated scratch test, Transwell assays blotting. The changes tumorigenic capacity transfected cells nude mice. highly expressed tissues negative correlation overall survival, survival after progression, relapse-free (all P<0.001). showed a positive (P= 0.00049) both significantly up-regulated (P<0.001). suggested involvement adhesion, migration, angiogenesis NF-κB (P<0.05). experiment, decreased while enhanced abilities positively regulated phosphorylated P65 (P-P65), VEGFA IL-17, blocking by PDTC lowered ability mice, resulted reduced tumor size mass, SPHK1-overexpressing tumorigenicity. regulates via may serve as potential diagnostic marker for progression.