[A rapid method for detecting

• DOI: 10.12122/j.issn.1673-4254.2025.02.20
• Publication Date: 2025-02-20
• Authors: Yi Yan; Jianyu Yang; Yujiao Zhou; Wenbo Ding; X. Zhang; Lijun Gao; Beizhen Pan; Jifei Yang; Yundong Zhao

To detect prfA and hly toxin genes of Listeria monocytogenes using polymerase chain reaction (PCR) colloidal gold technology. L. DNA was extracted by boiling method. With as the target genes, 5' ends upstream downstream primers gene were labeled with 6-FAM biotin, digoxin respectively, to establish detection Using cloning transformation, sequencing analysis, positive control products, kid developed its specificity, sensitivity, reproducibility stability tested, followed verification sample testing. The concentration method 148.81±0.97 ng/μL, A260/A280 ratio ranged from 1.8 2.0. PCR products showed a 100% homology sequences in GenBank database after cloning, transformation sequencing. strip yielded results only for samples without cross-reactions Staphylococcus aureus, Escherichia coli or Bacillus cereus, minimum limit 10-2 demonstrating 10-fold greater sensitivity test than agarose gel electrophoresis. also good when performed different operators strips storage 6 12 months. that this kit could accurately quickly L.monocytogenes samples. study can simultaneously use food safety inspection.