A5052044256- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Animal Disease Management and Epidemiology
- Insect and Pesticide Research
- Biosensors and Analytical Detection
- Viral Infections and Immunology Research
- Insect symbiosis and bacterial influences
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Plant Parasitism and Resistance
- Pharmacological Effects and Assays
- Animal Virus Infections Studies
- Sarcoma Diagnosis and Treatment
- Insect Pest Control Strategies
- Entomopathogenic Microorganisms in Pest Control
- Microbial infections and disease research
- Viral Infectious Diseases and Gene Expression in Insects
- Insect and Arachnid Ecology and Behavior
- Plant Virus Research Studies
- Bone Tumor Diagnosis and Treatments
- Toxin Mechanisms and Immunotoxins
- Antibiotics Pharmacokinetics and Efficacy
- Mosquito-borne diseases and control
Lanzhou Veterinary Research Institute
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Lanzhou University
2023-2025
Beihua University
2025
Henan Academy of Agricultural Sciences
2014-2024
Kunming Medical University
2017-2021
State Council of the People's Republic of China
2017-2019
Wuhan Ship Development & Design Institute
2019
South China Agricultural University
2019
Key Laboratory of Guangdong Province
2019
A rapid immunochromatographic lateral flow test strip of competitive format has been developed using a gold-conjugated monoclonal antibody for the specific determination enrofloxacin (ENR) residues in chicken muscles. For this purpose, (mAb) ENR was generated and characterized. The mAb showed negligible cross-reactivity with other related compounds. Using standards prepared muscle extracts from 0 to 24.3 ng/mL (μg/kg), method indicated that detection limit strip, as measured scanner, low...
Anaplasmosis is caused by obligate intracellular bacteria in the genus Anaplasma. These bacterial pathogens are transmitted ticks and impact both human animal health. This study was conducted to determine prevalence molecular characterization of Anaplasma spp. ruminants sampled Xinjiang, northwest China.A survey performed August 2012 rural areas six counties Xinjiang province. A total 250 blood samples from were collected tested for presence PCR. Positive genetically characterized based on...
A rapid immunochromatographic lateral flow test strip in a sandwich format was developed with the colloidal gold-labeled mouse antiglycinin monoclonal antibody (mAb) and rabbit polyclonal (pAb) to specifically identify glycinin, soybean allergen. The is composed of sample pad, conjugate reagent an absorbent membrane containing control line line. This has high sensitivity, results can be obtained within 10 min without sophisticated procedures. limit detection (LOD) calculated 0.69 mg/kg using...
Anaplasma and Ehrlichia are important emerging tick-borne pathogens in both humans animals. Here, we conducted a molecular surveillance study Gansu, China to assess the prevalence of spp. red deer sika based on polymerase chain reaction (PCR) analysis sequencing 16S rRNA or msp genes. PCR revealed that ovis, bovis platys Qilian Mountain samples was 32%, 9% 9%, respectively; bovis, 20%, 15% among Long samples, respectively. Of two (5%) 40 were positive for canis, but all 44 negative E. no...
African swine fever (ASF) has caused huge economic losses to the industry worldwide. Since there is no commercial ASF vaccine available, an early diagnosis extremely important prevent and control disease. In this study, virus (ASFV) capsid protein-encoding gene (p72) was selected used design primers for establishing a one-step visual loop-mediated isothermal amplification (LAMP) assay with neutral red, pH-sensitive dye, as color shift indicator. Neutral red exhibited sharp contrast of change...
A rapid immunochromatographic test strip has been developed for the detection of zearalenone (ZEN) residues in corn. For this purpose, a specific anti-ZEN monoclonal antibody (mAb), 4A3-F9, was obtained and identified. ZEN coupled to bovine serum albumin (BSA) via 1,4-butanediol diglycidyl ether prepared as immunogen. The mAb showed low cross-reactivity with five analogues. Using an preparation titer ≥1:5.12 × 10(5), (CR) four analogues <4%, except zearalanone, which 53.121%. recovery rates...
To date, anaplasmosis has been reported to be a subclinical disease in Indian and Arabian one-humped camels (Camelus dromedarius) llamas (Lama glama). However, no information on Anaplasma infection two-humped Bactrian bactrianus) China published date. The aim of this study was investigate the prevalence spp. domestic ticks Xinjiang, China. A total 382 were collected from environmental sources. Of these, 84 morphologically identified as belonging Rhipicephalus sanguineus group genetically...
Anaplasma spp. are tick-transmitted bacteria that infect a wide variety of wild and domestic animals. These pathogens exhibit high degree biological diversity, broad geographical distribution, represent serious threat to veterinary public health worldwide. A novel species was identified in Haemaphysalis qinghaiensis (Ixodidae) northwestern China molecularly characterized by comparison 16S rRNA, gltA, groEL gene sequences. Of the 414 samples tested, 24 (5.8%) were positive for this species....
Tick-borne diseases currently represent an important issue for global health. A number of emerging tick-transmitted microbes continue to be discovered, and some these are already identified as the cause human infections. Over past two decades, Anaplasma phagocytophilum is considered mainly responsible anaplasmosis. However, a novel zoonotic pathogen provisionally named "Anaplasma capra" has recently been in China. In this study, we did active surveillance A. capra goats sheep different...
Anaplasma species are tick-transmitted obligate intracellular bacteria that infect many wild and domestic animals humans. The prevalence of spp. in ixodid ticks Qinghai Province is poorly understood. In this study, a total 1104 questing adult were investigated for the infection species. As result, we demonstrated rates 3.1, 11.1, 5.6, 4.5% A. phagocytophilum, bovis, ovis capra, respectively. All tick samples negative marginale. positive capra different significantly different. bovis male...
African swine fever (ASF) is an acute and highly contagious disease that has caused great losses in the past years. It by virus (ASFV), which a large DNA encoding about 165 genes. been shown purified extracellular ASFV internalized both constitutive macropinocytosis clathrin-mediated endocytosis, utilizes apoptotic bodies for infection cell transmission. The ASFV-encoded RNA polymerase subunit C315R thought to play important role replication transcription. However, its involvement infection,...
ABSTRACT Multigene family (MGF) 360 genes, which are African swine fever virus (ASFV) virulence primarily target key host immune molecules to suppress interferon (IFN) production and interferon-stimulated gene (ISG) transcription, impairing innate responses for efficient viral replication. However, the interactions between MGF genes molecules, as well mechanisms through regulate IFN signaling, require further elucidation. In this study, we discovered that ASFV MGF_360-4L interacts with MDA5...
To detect prfA and hly toxin genes of Listeria monocytogenes using polymerase chain reaction (PCR) colloidal gold technology. L. DNA was extracted by boiling method. With as the target genes, 5' ends upstream downstream primers gene were labeled with 6-FAM biotin, digoxin respectively, to establish detection Using cloning transformation, sequencing analysis, positive control products, kid developed its specificity, sensitivity, reproducibility stability tested, followed verification sample...
A rapid immunochromatographic lateral flow test strip of competitive format has been developed for the specific determination olaquindox (OLA) residues in pig urine and muscle tissues. The sensitivity was found to be 1.58 ± 0.27 μg/kg 1.70 0.26 OLA tissues, lower detection limit 0.08 0.31 0.07 respectively. For negative samples spiked with 4, 12, 36 μg/kg, recovery range 83.0-94.0% 78.8-87.4% coefficient variation scope [CV (%)] 3.17-7.41% 4.66-7.64% Parallel analysis from tissue showed...
Theileria and Babesia protozoan parasites are transmitted mainly by tick vectors. These cause heavy economic losses to the live-stock industry, as well affecting health of wild animals in parasite-endemic areas. Identification infectious agents is not only crucial for species preservation, but also provides valuable information on parasite epidemiology. Here, we conducted a molecular surveillance study Northwestern China assess prevalence blood pathogens cervids. PCR analysis microscopic...