A rapid immunochromatographic lateral flow test strip of competitive format has been developed using a gold-conjugated monoclonal antibody for the specific determination enrofloxacin (ENR) residues in chicken muscles. For this purpose, (mAb) ENR was generated and characterized. The mAb showed negligible cross-reactivity with other related compounds. Using standards prepared muscle extracts from 0 to 24.3 ng/mL (μg/kg), method indicated that detection limit strip, as measured scanner, low...

Raf/MEK/ERK and phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) cascades are key signalling pathways interacting with each other to regulate cell growth tumourigenesis. We have previously shown B-Raf Akt overexpression and/or overactivation in pituitary adenomas. The aim this study is assess the expression their downstream components (MEK1/2, ERK1/2, mTOR, TSC2, p70S6K) effectors (c-MYC CYCLIN D1). studied tissue from 16 non-functioning adenomas (NFPAs), six...

Marek's disease virus (MDV) is a representative alpha herpes able to induce rapid-onset T-cell lymphoma in its natural host and regarded as an ideal model for the study of virus-induced tumorigenesis. Recent studies have shown that mdv1-miR-M4-5p, viral analog cellular miR-155, critical MDV׳s oncogenicity. However, precise mechanism whereby it was involved MD lymphomagenesis remained unknown. We presently identified mRNA targets mdv1-miR-M4-5 latent TGF-β binding protein 1 (LTBP1) target it....

The microRNA (miRNA) mdv1-miR-M4, a functional miR-155 ortholog encoded by oncogenic Marek's disease virus (MDV), has previously been suggested to be involved in MDV pathogenesis. Using the technique of bacterial artificial chromosome mutagenesis, we have presently evaluated potential role mdv1-miR-M4 oncogenesis very virulent (vv) strain GX0101. Unexpectedly, deletions Meq-cluster or alone from viral genome strongly decreased rather than abolished its oncogenicity. Compared GX0101,...

Porcine circovirus type 2 (PCV2) is the pathogen of porcine associated diseases (PCVAD) and one main pathogens in global pig industry, which has brought huge economic losses to industry. In recent years, there been limited research on prevalence PCV2 Henan Province. This study investigated genotype evolution this area.We collected 117 clinical samples from different regions Province 2015 2018. Here, we found that infection rate was 62.4%. Thirty-seven positive were selected amplify complete...

Marek’s disease virus (MDV) is an alphaherpesvirus associated with (MD), a highly contagious neoplastic of chickens. MD serves as excellent model for studying virus-induced T-cell lymphomas in the natural chicken hosts. Among limited set genes oncogenicity, MDV-miR-M4, expressed viral ortholog oncogenic miR-155, has received extensive attention due to its direct role induction lymphomas. Using targeted CRISPR-Cas9-based gene editing approach MDV-transformed lymphoblastoid cell lines, we show...

The avian immunosuppressive and neoplastic diseases caused by Marek’s disease virus (MDV), leucosis (ALV), reticuloendotheliosis (REV) are seriously harmful to the global poultry industry. In recent years, particularly in 2020–2022, outbreaks of such chicken flocks frequently occurred China. Herein, we collected live diseased birds from 30 farms, out 42 farms with tumour-bearing distributed central China, investigate current epidemiology co-infections these viruses. results showed that...

As one of the most important avian immunosuppressive and neoplastic diseases, Marek's disease (MD), caused by oncogenic virus (MDV), has huge economic losses worldwide over past five decades. In recent years, MD outbreaks have occurred frequently in MD-vaccinated chicken flocks, but key pathogenic determinants influencing factors remain unclear. Herein, we analyzed pathogenicity seven newly isolated MDV strains from tumor-bearing chickens China found that all them were to hosts, among which...

Marek's disease virus (MDV) is a highly pathogenic and oncogenic alpha herpesvirus that causes (MD), which one of the most important immunosuppressive rapid-onset neoplastic diseases in poultry. The onset MD lymphomas other clinical can be efficiently prevented by vaccination; these vaccines are heralded as first demonstration successful vaccination strategy against cancer. However, persistent evolution epidemic MDV strains towards greater virulence has recently resulted frequent outbreaks...

In the last decade, numerous microRNAs (miRNAs) have been identified in diverse virus families, particularly herpesviruses. Gallid alphaherpesvirus 2 (GaHV2) is a representative oncogenic that induces rapid-onset T-cell lymphomas its natural hosts, namely Marek’s disease (MD). GaHV2 genome there are 26 mature miRNAs derived from 14 precursors assembled into three clusters, Meq-cluster, Mid-cluster and LAT-cluster. Several miRNAs, especially those Meq-cluster (e.g. miR-M4-5p), demonstrated to...

A colloidal gold-based immunochromatographic strip has been developed for the rapid, simultaneous, semi-quantitative and quantitative detection of several aminoglycoside residues in milk, including gentamicin sulfate (GM), neomycin (NEO) kanamycin (KN).

Abstract BACKGROUND Fumonisins are a kind of toxic and carcinogenic mycotoxin. A rapid immunochromatographic test strip has been developed for simultaneous detection fumonisin B 1 , 2 3 ( FB ) in maize based on colloidal gold‐labelled monoclonal antibody McAb against probe. RESULTS The anti‐ 2E11‐H3 was produced through immunisation cell fusion, identified as high affinity, specificity sensitivity. cross‐reaction ratios with were accordingly 385% 72.4%, while none other analogues. colloid...

Recent advancements in biotechnology have enabled the rapid identification and subsequent expression of pathogenic microbial major antigens that induce protective immune responses. However, subunit vaccines not been successfully commercialized mainly due to lack sufficient levels neutralizing antibodies (NAs). High NA rely on efficient recognition cross-linking multiple epitopes with B-cell receptors (BCRs). Nanoparticles are able display coupled antigenic arrays at high density provide...

Marek's disease virus (MDV) is a long-recognized oncogenic herpesvirus, which induces lymphoma in its natural host that can be prevented by vaccination. MDV infection provides an excellent biological model for investigating the biology, genetics and immunology of viral oncogenesis. Recently discovered microRNAs (miRNAs) genome have been suggested to regulatory roles during We examined expression profiles all 22 previously reported miRNAs encoded MDV-1 chickens artificially challenged with...

Marek's disease virus (MDV) is an important oncogenic alphaherpesvirus that induces rapid-onset T-cell lymphomas in its natural hosts. The Meq-clustered miRNAs encoded by MDV have been suggested to play potentially critical roles the induction of lymphomas. Using technique bacterial artificial chromosome mutagenesis, we presently constructed a series specific miRNA-deleted mutants and demonstrate these are not essential for replication no effects on early cytolytic or latent phases...

Marek’s disease virus (MDV), a lymphotropic α-herpesvirus associated with T-cell lymphomas in chickens, is an excellent model for herpesvirus biology and virus-induced oncogenesis. (MD) also one of the cancers against which vaccine was first used. In lymphoblastoid cell lines (LCLs) derived from them, MDV establishes latent infection limited gene expression. Although LCLs are valuable interrogating viral host functions, molecular determinants maintenance latency lytic switch remain largely...

The virus-encoded microRNAs (miRNAs) have been demonstrated to important regulatory roles in herpesvirus biology, including virus replication, latency, pathogenesis and/or tumorigenesis. As an emerging efficient tool for gene editing, the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has successfully applied manipulating genomes of large DNA viruses. Herein, utilizing CRISPR/Cas9 with a double-guide RNAs transfection/virus infection strategy, we established...

To investigate the host-pathogen interactions between infectious bursal disease virus (IBDV) and target B-lymphocytic cells, a cDNA T7 phage display library from chicken bursa of Fabricius was constructed screened for binding. Surface immunoglobulin M (sIgM) isolated as putative candidate binding site its with IBDV were further investigated using lymphoma-derived cell line DT40. The results showed that λ light chain sIgM specifically interacted in virulence-independent manner vitro, most to...