Induksi Kalus dari Hipokotil Alfalfa ( medicago sativa l.) secara in vitro dengan Penambahan Benzyl Amino Purine (BAP) dan α-Naphtalene Acetic Acid (NAA)

• DOI: 10.14710/bioma.12.1.6-12
• Publication Date: 2010-01-01
• Authors: Surya Kurnia Hayati; Yulita Nurchayati; Nintya Setiari
• Keywords: Callus; Medicago sativa

Alfalfa (Medicago sativa L.) is useful plant for treatment some diseases such as: cancer, diabetes, lupus, and hepatitis. Propagation of this in Indonesia face a problem which has no embryo. One method to propagate by tissue culture or micropropagation. Callus induction first step micropropagation produce callus will be regenerated become planlet. The aims research are induce callogenesis from hipocotyl alfalfa with addition Benzyl Amino Purine (BAP) α Naphtalene Acetic Acid (NAA), determine the proper combination BAP NAA optimal callus. experiment been conducted using 12 Completely Randomized Design (CRD) 4x3 factorial pattern 5 replicates. Data were analyzed ANOVA 95% Degrees Freedom (DF). If there was significance result, it followed DMRT at 95 % DF. result showed that able alfalfa. obtained 0 ppm 2 ppm.