Cymbidium bicolor is a highly hunted and traded orchid, leading to decline in its wild population. Orchid conservation can be achieved through tissue culture, particularly via somatic embryogenesis. Thidiazuron (TDZ) growth regulator used induce This study aimed determine the optimal TDZ concentration for embryo formation. Stem explants of C. were cultured on Murashige Skoog (MS) medium with concentrations 0, 1, 2, 3 ppm. Observations conducted weekly two months using stereo microscope...

Binahong (Basella rubra L.) is a plant medicine consisting secondary metabolites which have virtue as medicines for several diseases that could also be used coloring agent. The compounds in extracted from callus. Sucrose one of the components build MS (Murashige & Skoog) medium. important vitro culture, it functions carbon and energy source explant to grow. purposes this research are study effect sucrose medium towards B. L callus formation growth; find optimum concentration formation;...

Germination and growth of orchid seeds can be accelerated by the addition organic supplement plant extract in culture medium. The objective this study was to determine effect peptone tomato on early stage embryo development Dendrobium phalaenopsis orchids. Orchid were sown NP VW medium with 10% CW (NPCW VWCW). Five weeks after seed germination, about 58.03% germination observed VWCW medium, only 37.45% NPCW. Tomato added VWCW, resulting VWCWTP After 4-8 VWCWTP, 94.42% germinated into...

Chlorophyll is a pigment of photosynthesis which also efficacious as cure for brain, lung, and oral cancer, can be used disinfectant, antibiotic dietary supplement, while carotenoids are useful antioxidants. The content the two pigments strongly influenced by age plant, especially vegetable crops in leaves such cowpea (Vigna unguiculata (L.) Walp.). purpose this research to know pattern chlorophyll carotenoid leaf during growth phase right plant harvesting so that highest obtained. Cowpea...

To increase the efficiency of crop production from Dendrobium phalaenopsis orchids, mass propagation has been performed by inducing somatic embryogenesis through Agrobacterium-mediated transformation Arabidopsis embryo gene AtRKD4 into orchid protocorm (developing embryo). The three-week-old protocorms D. were genetically transformed with T-DNA carrying 35S :: GAL4 GR A. tumefaciens strain EHA 105. cultures maintained in VW medium 10 mg L-1 Hygromycin. Due to existence glucocorticoid...

Chlorophyll is a plant pigment which recently have been popular as food supplement. The source of chlorophyll consumed now are alfalfa’s leaves algae such Spirulina and Chlorella.. content find in all kinds especially vegetables so that potent to be chlorophyll. aims this research explore alternative aside Leguminosae, from sweet basil, spinach, water cassava leaves, pegagan, papaya’s study the experiment has conducted by extracting 0,1 g each 10 mL acetone 85%. extract was filtered with...

Waterlogging is a shallow flooding in the area of root and some parts shoot. It one most common types agricultural areas. The duration waterlogging affects plant growth yield response to stress by interacting with their ability adapt. Plant adaptability during resilience post-waterlogging recovery conditions. In this research, we examined tolerance Capsicum frutescens short (1 day), medium (3 days) long (10 waterlogging, as well its implications on post-waterlogging, recovery, reproductive...

This paper studies the effect of plant growth regulators, i.e., indol butyric acid (IBA), to root in stem cutting technique P. cablin, and correct concentration IBA obtain optimum growth. The research were carried out Lab Bio Struktur dan Fungsi Tumbuhan, UNDIP, Semarang. Complete Randome Design was used as method which 4 different concentrations, control 0, 25, 50 75 ppm, observed with parameter amount root, length wetted weight, dried efficiency. results show immersing into 25 ppm exhibits highest

Alfalfa (Medicago sativa L.) is useful plant for treatment some diseases such as: cancer, diabetes, lupus, and hepatitis. Propagation of this in Indonesia face a problem which has no embryo. One method to propagate by tissue culture or micropropagation. Callus induction first step micropropagation produce callus will be regenerated become planlet. The aims research are induce callogenesis from hipocotyl alfalfa with addition Benzyl Amino Purine (BAP) α Naphtalene Acetic Acid (NAA), determine...

Teknologi kultur jaringan menjadi alternatif teknologi yang mampu menyediakan bibit secara massal, seragam dan relatif cepat. Multiplikasi tunas dari pucuk merupakan tahap untuk mendukung pembentukan planlet dengan penambahan zat pengatur tumbuh (ZPT) sitokinin. Tujuan penelitian ini adalah mengkaji pengaruh ZPT Benzil Aminopurin (BAP) thidiazuron (TDZ) terhadap alfalfa pada media serta mengetahui konsentrasi kedua dalam membentuk optimal. Kultur diperoleh kecambah aseptik berumur 10 hari,...

Jatropha is an alternative resource of sustainable energi. Therefore, to support the use energi,an increase production seed important. As seeds (fruits) only growth at terminal andaxial bud, therefore presence lateral bud necessary seed.Formation affected by balance between auxin and sitokinin. Defoliation will control thebalance these two hormone. In this study, defoliation conducted on seedling age 4 month.Each treatment was replicated  7 times. Variabel were observed ended after 6 month....

Abstract Macroalgae, such as Sargassum and Gracilaria have a great potential to be used soil conditioner. Addition of macroalgae powder into certain type could significantly change fertility. The presence gelatinous material in are predicted able changing the chemical characteristic soil, including organic content, pH C/N ratio. This study was focused on evaluation fertility sandy clay after addition collected macroalga dried soaked fresh water several time lower its salinity reach normal...

Dendrobium phalaenopsis Fitzg. is an ekndemic orchid from Larat island (Maluku) Eastern Indonesia which classified as endangered plant. Therefore, mass propagation to conserve this plant needs be conducted. For rapid propagation, Arabidopsis AtRKD4 gene has been introduced into protocorm of D. by using Agrobacterium- mediated transformation with T-DNA containing 35S::GR::AtRKD4 construct. Dexamethasone (DEX) was used activate the transgene AtRKD4. The leaf one year-old plantlets were cut and...

Patchouly is one of the plants that produce essential oil, i.e. patchouly which commonl  used in cosmetic industry. Increasing biomass by triggering growth could be way to increase production pathouly oil. Therefore, purpose this research study effect apical bud cutting towards growth, and also understand optimum location on nodus. The Complete Random Design method with 5 repetitions. There were 3 locations (R), R0 (without cutting), R1 (cutting at first nodus), R2 second nodus). observed...

<p><em>Vanda limbata</em> is a natural orchid with slow growth and often taken directly from the forest in large quantities, threatening its sustainability. Ex-situ preservation efforts are needed for this orchid, one of which through cultivation addition Benzyl Amino Purin (BAP), known to increase shoot growth. This research aims analyze effect BAP determine optimal concentration on <em>V. orchids. used single-factor completely randomized design concentrations 0, 25...

Kualitas jahe merah ditentukan oleh kandungan minyak atsiri yang mudah menguap. Kombinasi cabinet solar dryer (CSD) sebagai alat pengering dikombinasikan dengan kain penutup dapat mempengaruhi rendemen atsiri. Penelitian ini bertujuan untuk mempelajari pengaruh kombinasi metode pengeringan penambahan terhadap merah. menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2 x 3 ulangan. Faktor pertama adalah dan terbuka tanpa alat. kedua kain, 1 lapis penutup. Parameter diamati volume atsiri,...

High salinity causes osmotic stress and ion imbalance that can reduce plant productivity. Solanaceae be developed for cultivation in saline land, but its growth is influenced by the type of species. This study aims to examine tolerance level three plants through observation physiological responses germination growth. used a 3 x 4 factorial Completely Randomized Design (CRD). The first factor salinity: 0 ppm, 2,500 5,000 ppm 7,500 ppm. second species, namely Capsicum frutescens, Solanum...

Red okra fruit has high nutritional value and fiber but still low production. Monosodium glutamate (MSG) consists of sodium glutamic acid. In plants, can role replacing potassium, stomata physiology, chlorophyll biosynthesis. Glutamate as the nitrogen donor in primer metabolism gibberellic acid precursor. The assumption that MSG plants a positive impact. This research aimed to examine effect optimum dosage for enhances growth was conducted with Completely Randomized Designs (CRD) one factor,...

Crystal guava (Psidium guajava var. 'Crystal') fruit is in great demand because of its delicious taste and high nutritional content. Storage aims to prevent postharvest damage the fruit. However, storage that too long causes morphological decreased nutrients. This study determine effect duration, ripeness stage interaction between both factors on vitamin C total soluble solids (TSS) content crystal guava, which treatment can produce highest TSS. Fruits harvested simultaneously with three...

Jambu kristal (Psidium guajava L. cv. ‘Kristal’) memiliki daging buah berwarna putih, tekstur renyah, tidak banyak biji serta kandungan gizi, salah satunya vitamin C. Buah jambu yang sudah dipanen umumnya dikemas sebelum didistribusikan dengan tujuan untuk meminimalisir kerusakan maupun degradasi C selama penyimpanan. Penelitian ini bertujuan mengetahui pengaruh jenis pengemas berbeda terhadap dan morfometri kristal. pada umur 100 HSA (Hari Setelah Antesis), berdasarkan rentang ukuran bobot...