ABSTRACT The asymmetric leaves2 (as2) mutant of Arabidopsis thaliana generated leaf lobes and leaflet-like structures from the petioles leaves in a bilaterally manner. Both delayed formation primary vein secondary veins were apparent primordia as2 plants. A distinct midvein, which is thickest located longitudinal center lamina wild-type plants, was often rudimentary even mature leaves. However, several parallel very similar thickness evident such complexity venation patterns all leaf-like...

The ASYMMETRIC LEAVES2 (AS2) gene of Arabidopsis thaliana is involved in the establishment leaf venation system, which includes prominent midvein, as well development a symmetric lamina. product also represses expression class 1 knox homeobox genes leaves. We have characterized AS2 gene, appears to encode novel protein with cysteine repeats (designated C-motif) and leucine-zipper-like sequence amino-terminal half primary sequence. genome contains 42 putative that potentially proteins...

Faculty of Mathematics and Natural Science, Gadjah Mada University, Kaliurang Street, Sekip Utara, Yogyakarta 55281, Indonesia. *Corresponding author: trijr_mipa@ugm.ac.id ABSTRACT Keywords: Degenerate primer, primaclade, in silico, colony PCR, Azospirillum sp. JG3 INTRODUCTION To answer many questions evolutionary developmental biology, it is necessary to identify genes which could be responsible for the studied traits (Žlůvova, 2007). PCR amplification homologous using degenerate primers...

Summary The ASYMMETRIC LEAVES2 ( AS2 ) gene, a member of the / LOB gene family, and LEAVES1 AS1 Arabidopsis thaliana participate in development symmetrical, expanded lamina. We report here patterns expression these genes, importance sites such leaf development. Transcripts both genes accumulated entire primordia at early stages, but accumulation changed as leaves expanded. transcripts were detected, respectively, adaxial domain inner between abaxial domains leaves. ratios numbers cells to...

Eria hyacinthoides (Blume) Lindl. is an Indonesian orchid species found in Sumatra, Java, and Bali. This a sympodial with flowers that has fragrant aroma, suspected containing phytochemicals for herbal medicines, so mass plant propagation necessary. The aim of this research to obtain the best vitro conditions through somatic embryos using growth regulators analysing structure Dendrobium Orchid Homeobox 1 (DOH1) homologous gene E. ‘Madame Thong-In’ which known induce bud formation. method...

Phalaenopsis hybrids constitute a major ornamental crop. An important parent species for many of these is amabilis. We developed convenient method the genetic modification P. amabilis using Agrobacterium tumefaciens. The transformed intact protocorms, which are young orchid seedlings amabilis, regenerated plants under same conditions that showed highest frequency shooting. A kanamycin resistance gene control 35S promoter can be used as selective marker. In addition, T-DNA vectors containing...

Phalaenopsis amabilis is an important national flower of Indonesia as a parent for orchid breeding, so that needs good strategy to produce high number plants. The objective this research analyze the use thidiazuron (TDZ) producing plantlets, through directly induction somatic embryos (SEs) from various explants. method was used 20 each protocorms, leaves, stems and roots explants were dissected transversely, then put on culture media: New (NP) NP + (1, 2, 3) mgL−1 TDZ. Cultures maintained at...

We have investigated physical distances and directions of transposition the maize transposable element Ac in Arabidopsis thaliana. prepared a transferred DNA (T-DNA) construct that carried non-autonomous derivative with site for cleavage by endonuclease I-SceI (designated dAc-I-RS element). Another was also introduced into T-DNA region outside dAc-I-RS. Three transgenic plants were generated, each which had single copy at different chromosomal location. These crossed gene transposase progeny...

Phalaenopsis “Sogo Vivien “is a mini orchid hybrid with beautiful flowers and numerous inflorescences.Mass propagation of this is needed to meet the market demand. Objective research was toinduce somatic embryogenesis P.”Sogo Vivien” through insertion AtRKD4 gene into orchid. T-DNAcontaining 35S::GAL4::AtRKD4::GR inserted 16-22 days after sowing protocorms mediated byAgrobacterium tumefaciens EHA 105. Activation induced by glucocorticoid inductionsystem, using 15μM Dexamethasone (Dex). The...

SummaryThe development of an efficient methodology for the genetic transformation orchids is needed in order to support thegenetic engineering orchids. It therefore important identify those factors affecting process.Previously, we reported a convenient method Phalaenopsis amabilis using Agrobacterium tumefaciens, which intact protocorms were used. We also found that embryos cultured on medium containing tomato extract grew more rapidly than with coconut water. When used grown extract,...

Orchid is an important ornamental plant in Indonesia due to their natural beauty of flowers. In the tropical forest, orchids are being acquired for trading and commercial market. Thus, effort required proliferate orchid large quantities conservation improve floral variation breeding. The purpose this study develop a firmed methodology molecular breeding using CRISPR/Cas9 KO system. material used was Phalaenopsis amabilis protocorms growth on NP medium+pepton (2 g/L). Protocorm were submerged...

Germination and growth of orchid seeds can be accelerated by the addition organic supplement plant extract in culture medium. The objective this study was to determine effect peptone tomato on early stage embryo development Dendrobium phalaenopsis orchids. Orchid were sown NP VW medium with 10% CW (NPCW VWCW). Five weeks after seed germination, about 58.03% germination observed VWCW medium, only 37.45% NPCW. Tomato added VWCW, resulting VWCWTP After 4-8 VWCWTP, 94.42% germinated into...

Micropropagation of AS2K clones Arabica coffee (Coffea arabica L.) was attempted through indirect somatic embryogenesis by using ten different parts the leaf such as shoot, first base, second third middle, tip, and tip. The influence part explants, combination plant growth regulator (PGRs) in induction embryogenic callus regeneration embryo were studied. Furthermore, various protocols to induce into plantlet step subculture use germination medium demonstrated. morphological characteristics...

Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germplasm not been extensively programs, primarily relation to improve drought tolerance. The objectives of experiment were evaluate the effect stress initiated at vegetative growth stage on growth, leaf proline content protein pattern seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, BOT-88). plants propagated from single node stalk...

To increase the efficiency of crop production from Dendrobium phalaenopsis orchids, mass propagation has been performed by inducing somatic embryogenesis through Agrobacterium-mediated transformation Arabidopsis embryo gene AtRKD4 into orchid protocorm (developing embryo). The three-week-old protocorms D. were genetically transformed with T-DNA carrying 35S :: GAL4 GR A. tumefaciens strain EHA 105. cultures maintained in VW medium 10 mg L-1 Hygromycin. Due to existence glucocorticoid...

Modern biotechnology for orchid breeding, especially in vitro culture and transgenic technology mass propagation has developed. Due to its attractive flower, Indonesian native orchids such as moth Phalaenopsis amabilis (L.) Blume, Black Coelogyne pandurata Lindley, Vanda tricolor Lindley are often taken from their natural habitat be traded, or domesticated a hobby. So the existence of Indonesia continues decline. integrated with classical breeding will support conservation strategy well...

Sandalwood (Santalum album Linnaeus), an endemic species of the East Nusa Tenggara province in Indonesia, is a timber producing forest plant. The population this plant now threatened into extinction because over-exploitation from their habitat. Micropropagation will be best way to solve problem. objective experiment determine medium for micropropagation and effect growth regulators on vitro culture Sandalwood. This was conducted three steps: i) In seed germination Murashige Skoog (MS) Woody...

Glagah (wild sugarcane, Saccharum spontaneum) is a perennial grass that grows well in marginal soils. It has high carbohydrate and fibre content, making it suitable for ethanol production the study industry. Thus, glagah potential as crop species its own right may also be used sugarcane breeding programs. However, germplasm not been extensively utilised programs, especially relation to improving drought tolerance. This was performed evaluate effect of stress over an eight week period on...

Abstract Alcaligenes faecalis , is an opportunistic, pathogenic, Gram‐negative, food‐borne bacterium appearing to be of public health importance due its resistance common antibiotics and frequent involvement in nosocomial infections water contamination. Yet, the Betaproteobacterium showed potential benefit fat degradation food waste. It suggested that pterin‐based Molybdenum cofactor (Moco) biosynthesis involving moa gene related with pathogenesis several Gram‐negative bacteria, while...

Abstract. Aloysius S, Purwantoro A, Dewi K, Semiarti E. 2017. Improvement of genetic variability in seedlings Spathoglottis plicata orchids through X-ray irradiation. Biodiversitas 18: 20-27. Developing via mutation is promising for orchid breeding. The objective this research was to improve variation X-ray-irradiation the seeds. method involved irradiation one month old mature seeds at doses 0, 6, 12, 18, and 24 rad. irradiated were sown on a half strength MS medium grown into protocorms...

<p>Orchids are generally cultivated for their flower. To induce flower initiation in Phalaenopsis amabilis orchid, genetic and physiological approaches were developed. Genetic modification by insertion of P. Flowering Locus T (PaFT) gene driven Ubiquitin promoter into orchid genome using Agrobacterium tumefaciens, whereas approach was conducted the use growth regulators: N6­benzyladenine (BA) (1, 3, 9) mg.L-1 or gibberellic acid (GA3) (5, 10, 15) alone combination culture medium....

Background and Objective: Dragon fruit (Hylocereus spp.), apple (Malus sylvestris Mill.) tomato (Solanum lycopersicum L.) are high potential sources of antioxidant compounds such as phenolics.The have the capability protecting cells tissues against free radicals.Secondary metabolite produced by callus cell culture from plant organs also acts a source antioxidants.This study aimed to determine optimal ratio sucrose 2,4-D in Murashige Skoog (MS) medium for induction different organ...