Phalaenopsis hybrids constitute a major ornamental crop. An important parent species for many of these is amabilis. We developed convenient method the genetic modification P. amabilis using Agrobacterium tumefaciens. The transformed intact protocorms, which are young orchid seedlings amabilis, regenerated plants under same conditions that showed highest frequency shooting. A kanamycin resistance gene control 35S promoter can be used as selective marker. In addition, T-DNA vectors containing...

Phalaenopsis amabilis is an important national flower of Indonesia as a parent for orchid breeding, so that needs good strategy to produce high number plants. The objective this research analyze the use thidiazuron (TDZ) producing plantlets, through directly induction somatic embryos (SEs) from various explants. method was used 20 each protocorms, leaves, stems and roots explants were dissected transversely, then put on culture media: New (NP) NP + (1, 2, 3) mgL−1 TDZ. Cultures maintained at...

The research was conducted to determine the morphological characteristics, productivity andquality of Napier grass cultivars Taiwan, King and Mott harvested at 8 13 after planting. Effect ofcultivar harvest arranged in a nested design (3x2). variables were observed consisting ofmorphological dry matter production forage quality. results showed that themorphological leaf stem ratio, fiber content, crude protein content vitro matterdigestibility Taiwan cultivar not different, but both...

SummaryThe development of an efficient methodology for the genetic transformation orchids is needed in order to support thegenetic engineering orchids. It therefore important identify those factors affecting process.Previously, we reported a convenient method Phalaenopsis amabilis using Agrobacterium tumefaciens, which intact protocorms were used. We also found that embryos cultured on medium containing tomato extract grew more rapidly than with coconut water. When used grown extract,...

The purpose of this research was to evaluate the growth phase orchid Vanda hybrid (Vanda limbata Blume X tricolor Lindl. var. suavis) response with colchicines treatment, determine precise effective concentration and duration treatment induce polyploidy. This used variation which were 0.01%; 0.05%; 0.1%; 0.5%; 1% 0% incubation for 6 hours; 12 18 24 4 days. Treatment done in aseptic condition using protocorm aged 7 week after sowing. After treatments, plants planted on Vacint Went (VW) medium...

Background and Objective: Stelechocarpus burahol is a plant containing flavonoid compounds that have the potential for use as an antihyperuricemic gout medication.This study was performed to assess production, growth cell differentiation of S. in suspension culture.Methodology: Mesocarp planted Murashige Skoog (MS) medium supplemented with 7.5 mg LG 1 picloram induction callus.Non-embryonic callus obtained used formation cultures.Growth cells determined by fresh dry weights.During culturing,...

<p><em>Stelechocarpus burahol </em>is one of the medicinal plants that contains flavonoids. The study was carried out to know flavonoid production cultures in vitro <em>S. </em>from mesocarp explants. Mesocarp explants were cultured on MS medium containing different combination and concentration plant growth regulators i.e. picloram (5, 7.5 10 mg/L) 2, 4-D (10, 15 20 under dark condition. Induction callus formation started 20.29<sup>th</sup>...

Sandalwood (Santalum album L.) is native species of Indonesia, especially in East Nusa Tenggara, oneof the twenty two genus Santalum world. an important tree because it hashigh economic value can produce sandal oil these be used for perfumes, cosmetics, pharmaceuticals, andare often religious ceremonies. In vitro particularly somatic embryogenesis has been widely appliedin propagation sandalwood. The Objective this research to obtain regeneration sandalwoodthrough using leaves explant from...

Our previous study showed that kaffir lime leaf has anti-cancer compounds. Elicitation in cell suspension could increase the amount of In order to develop suspension, a friable callus as raw material is needed. Therefore, objective this was determine best concentration 2,4-D produce and make culture from callus. study, we used Murashige Skoog (MS) media with various concentrations (0 ppm, 2 5 10 ppm). Calli were incubated for 50 days. The parameters measured biomass, color, texture. Criteria...

Cahaya merah dan biru merupakan cahaya utama yang diserap tanaman berperan penting terhadap fotosintesis, pertumbuhan serta perkembangan anggrek Phalaenopsis hibrida. Cekaman dingin mampu memicu pembentukan organ generatif memperlambat vegetatif. Namun, pengaruh intensitas rendah cekaman vegetatif belum diketahui. Penelitian ini bertujuan untuk mengetahui warna suhu Anggrek berumur ± 2 tahun ditumbuhkan dalam growth chamber dengan penyinaran lampu LED putih sebagai kontrol (100 lx), merah,...

Perbaikan teknik perbanyakan dan mutu bibit klonal karet (Hevea brasiliensis Muell Arg) dapat dilakukan melalui kultur jaringan. Kendala tersebut diantaranya masih tingginya intensitas browning pada tahap inisiasi kalus. Browning umumnya disebabkan oleh senyawa fenolik yang biasanya muncul terakumulasi ketika eksplan dilukai. Penelitian ini bertujuan mengetahui metode eliminasi efektif diantara perlakuan perendaman dalam asam askorbat, arang aktif+sukrosa, subkultur berulang diinkubasi ruang...

To improve the growth of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet, a photoautotrophic micropropagation system (PMS) was developed by growing plantlet on VW medium with varying concentration sucrose (0, 5, 10, and 20 g/L) additional carbon dioxide from air (bottle covered cap or filter). The result showed that leaf length would increase up to 6.5 cm PMS it keep adding 5 g/L sucrose. Average number leaves increased 6.7 strands addition average quantity 7.7 strands. root even...

Orchid is the best tourism icon which focused on nature-based development in Indonesia. Banyunganti Hamlet one of villages Kulon Progo has a high diversity orchid species. Regarding this situation, guiding and assistance for villagers introduction natural species its character, conventional propagation, modern propagation by using household-scale tissue culture techniques (sowing seeds planting) important to give. The long-term goal activity was empowering women Dusun line with opening New...

<p>This experiment was aimed to evaluate the copper accumulation using callus culture of <em>Datura metel</em> L. The established from leaves onto MS contained NAA 2.5 mg/L and Kinetin 0.5 as control. exposure carried out by 2 compounds treatment, i.e. CuCl<sub>2</sub>.2H<sub>2</sub>O Na<sub>2</sub>CuEDTA at level concentration 0.; 0.1; 5; 10; 15; 20 µM. growth pattern in control showed increasing rate 36 days, whereas exponential stage...

Perbaikan teknik perbanyakan dan mutu bibit klonal karet (Hevea brasiliensis Muell Arg) dapat dilakukan melalui kultur jaringan. Kendala tersebut diantaranya masih tingginya intensitas browning pada tahap inisiasi kalus. Browning umumnya disebabkan oleh senyawa fenolik yang biasanya muncul terakumulasi ketika eksplan dilukai. Penelitian ini bertujuan mengetahui metode eliminasi efektif diantara perlakuan perendaman dalam asam askorbat, arang aktif+sukrosa, subkultur berulang diinkubasi ruang...

In vitro culture of orchids has been developed for many purposes. Some native and commercial orchid hybrids are propagated using seed germination or cut explants such as leaves, shoot tips, roots to produce large numbers plantlets. This technique is widely used the purpose in conservation natural species industry hybrids. However, molecular genetic mechanism behind growth development these during still unclear, needs be elaborated. Recent advanced transgenic technology very helpful studying...

Biotic stress by Odontoglossum ringspot virus (ORSV) infection causes a decrease in the rate of photosynthesis. This study aimed to determine pattern plant-virus interactions through analysis rbcL and coat protein genes. Virus source was collected from Bogor Botanical Garden. Detection

Aim: The existence of Orchid Mycorrhizal Fungi (OMF) has a role to stimulate growth and support the supply orchid nutrition as biofertilizer agent. This study aimed determine association mycorrhizal with Phalaenopsis amabilis (L.) Blume which was carried out through effectiveness test two Indonesian isolates i.e. Ceratorhiza Trichoderma.
 Study Design: consisted 4 treatments. Each treatment repeated 3 times, each repetition 5 plantlets, so that total plantlet used 60.
 Place...

Dendrobium capra is an Indonesian endemic orchid species that live in Java. It grows on low altitude with warm climate. D. has beautiful small yellow greenish flower grow raceme inflorescence. This faces a threat its natural habitat due to having long life cycle and forestry main commodity as host thus categorized Appendix II CITES list. To address problem, ex situ conservation approach using vitro culture method necessary. Germination enhancement effort complex organic substances found 200...