A5009646285- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Cancer-related Molecular Pathways
- Plant-Microbe Interactions and Immunity
- Genomics and Chromatin Dynamics
- Plant Virus Research Studies
- Mycorrhizal Fungi and Plant Interactions
- Tree Root and Stability Studies
- Cancer, Hypoxia, and Metabolism
- Leaf Properties and Growth Measurement
- Genetic Mapping and Diversity in Plants and Animals
- Agriculture and Biological Studies
- Chromosomal and Genetic Variations
- Antioxidant Activity and Oxidative Stress
- Plant Genetic and Mutation Studies
- Plant and fungal interactions
- Spaceflight effects on biology
- Chemical Reactions and Isotopes
- Fungal Biology and Applications
Kanazawa University
2022-2024
Max Planck Institute for Plant Breeding Research
2019-2024
Chubu University
2007-2023
Nara Institute of Science and Technology
2005-2022
Purdue University West Lafayette
2013
Nagoya University
2001-2007
ABSTRACT The asymmetric leaves2 (as2) mutant of Arabidopsis thaliana generated leaf lobes and leaflet-like structures from the petioles leaves in a bilaterally manner. Both delayed formation primary vein secondary veins were apparent primordia as2 plants. A distinct midvein, which is thickest located longitudinal center lamina wild-type plants, was often rudimentary even mature leaves. However, several parallel very similar thickness evident such complexity venation patterns all leaf-like...
The ASYMMETRIC LEAVES2 (AS2) gene of Arabidopsis thaliana is involved in the establishment leaf venation system, which includes prominent midvein, as well development a symmetric lamina. product also represses expression class 1 knox homeobox genes leaves. We have characterized AS2 gene, appears to encode novel protein with cysteine repeats (designated C-motif) and leucine-zipper-like sequence amino-terminal half primary sequence. genome contains 42 putative that potentially proteins...
Summary The ASYMMETRIC LEAVES2 ( AS2 ) gene, a member of the / LOB gene family, and LEAVES1 AS1 Arabidopsis thaliana participate in development symmetrical, expanded lamina. We report here patterns expression these genes, importance sites such leaf development. Transcripts both genes accumulated entire primordia at early stages, but accumulation changed as leaves expanded. transcripts were detected, respectively, adaxial domain inner between abaxial domains leaves. ratios numbers cells to...
Summary The protein kinase cdc2 is conserved throughout eukaryotes and acts as a key regulator of the cell cycle. In plants, A‐type cyclin‐dependent (CDKA), homologue cdc2, has role Here we show that loss‐of‐function mutation in CDKA;1 , encoding only Arabidopsis CDKA, results lethality male gametophyte. Heterozygous plants produced mature siliques containing about 50% aborted seeds, segregation distortion was observed paternal inheritance. Microspores normally undergo an asymmetric...
The ASYMMETRIC LEAVES2 (AS2) gene is required for the generation of flat and symmetrical shape leaf lamina in Arabidopsis. AS2 encodes a plant-specific protein with an AS2/LATERAL ORGAN BOUNDARIES (AS2/LOB) domain that includes cysteine repeat, conserved single glycine residue leucine-zipper-like sequence its amino-terminal half. Arabidopsis genome contains 42 genes, including AS2, encode proteins AS2/LOB their halves, these genes constitute family. In present study, we cloned characterized...
Leaf primordia are generated at the periphery of shoot apex, developing into flat symmetric organs with adaxial-abaxial polarity, in which indeterminate state is repressed. Despite crucial role ASYMMETRIC LEAVES1 (AS1)-AS2 nuclear-protein complex leaf polarity specification, information on mechanisms controlling their downstream genes has remained elusive. We systematically analyzed transcripts by microarray and chromatin immunoprecipitation assays performed genetic rescue as1 as2 phenotypic...
Pattern-recognition receptor (PRR)-triggered immunity (PTI) wards off a wide range of pathogenic microbes, playing pivotal role in angiosperms. The model liverwort Marchantia polymorpha triggers defense-related gene expression upon sensing components bacterial and fungal extracts, suggesting the existence PTI this plant model. However, molecular putative M. significance bryophytes have not yet been described. We here show that has four lysin motif (LysM)-domain-containing homologs, two...
Abstract We show that two Arabidopsis thaliana genes for histone deacetylases (HDACs), HDT1/HD2A and HDT2/HD2B, are required to establish leaf polarity in the presence of mutant ASYMMETRIC LEAVES2 (AS2) or AS1. Treatment as1 as2 plants with inhibitors HDACs resulted abaxialized filamentous leaves aberrant distribution microRNA165 and/or microRNA166 (miR165/166) leaves. Knockdown mutations these by RNA interference phenotypes like those observed background. Nuclear localization overproduced...
The NPR proteins function as salicylic acid (SA) receptors in Arabidopsis thaliana. AtNPR1 plays a central role SA-induced transcriptional reprogramming whereby positively regulates SA-mediated defense. NPRs are found the genomes of nearly all land plants. However, we know little about molecular functions and physiological roles most plant species. We conducted phylogenetic alignment analyses from 68 species covering significant lineages To investigate bryophyte lineages, generated...
Abstract The evolution of adaptive interactions with beneficial, neutral and detrimental microbes was one the key features enabling plant terrestrialization. Extensive studies have revealed conserved unique molecular mechanisms underlying plant–microbe across different species; however, most insights gleaned to date been limited seed plants. liverwort Marchantia polymorpha, a descendant early diverging land plants, is gaining in popularity as an advantageous model system understand...
Agrobacterium-mediated transient gene expression is a rapid and useful approach for characterizing functions of products in planta. However, the practicability method model liverwort Marchantia polymorpha has not yet been thoroughly described. Here we report simple robust transformation thalli its applicability. When M. were co-cultured with Agrobacterium tumefaciens carrying β-glucuronidase (GUS) genes, GUS staining was observed primarily assimilatory filaments rhizoids. activity detected 2...
The ASYMMETRIC LEAVES2 (AS2) gene in Arabidopsis thaliana is responsible for the development of flat, symmetric, and extended leaf laminae their vein systems. AS2 protein a member plant-specific AS2/LOB family, which includes 42 members comprising conserved amino-terminal domain referred to as domain, variable carboxyl-terminal region. Among members, has been most intensively investigated on both genetic molecular levels. forms complex with myb AS1, involved epigenetic repression abaxial...
SummaryThe development of an efficient methodology for the genetic transformation orchids is needed in order to support thegenetic engineering orchids. It therefore important identify those factors affecting process.Previously, we reported a convenient method Phalaenopsis amabilis using Agrobacterium tumefaciens, which intact protocorms were used. We also found that embryos cultured on medium containing tomato extract grew more rapidly than with coconut water. When used grown extract,...
Maintaining stable and transient quiescence in differentiated stem cells, respectively, requires repression of the cell cycle. The plant RETINOBLASTOMA-RELATED (RBR) has been implicated maintenance, presumably by forming repressor complexes with E2F transcription factors. Surprisingly we find that mutations all three canonical E2Fs do not hinder cycle, but similarly to RBR silencing, result hyperplasia. Contrary growth arrest occurs when exit from proliferation differentiation is inhibited...
Abstract The NPR proteins function as salicylic acid (SA) receptors in Arabidopsis thaliana . AtNPR1 plays a central role SA-induced transcriptional reprogramming whereby positively regulates SA-mediated defense. NPRs are found the genomes of nearly all land plants. However, we know little about molecular functions and physiological roles most plant species. Our phylogenetic alignment analyses show that Brassicaceae NPR1-like have characteristically gained or lost functional residues motifs...
Agrobacterium-mediated transformation is a core technology for basic plant science and agricultural biotechnology. Improving frequency major goal transgenesis. We previously showed that T-DNA insertions in some histone genes decreased susceptibility, whereas overexpression of several Arabidopsis H2A H4 isoforms increased transformation. Overexpression H2B H3 had little effect on frequency. However, H3-11 (HTR11) enhanced HTR11 unique variant lacks lysine at positions 9 27. The modification...
The ASYMMETRIC LEAVES2 (AS2) gene in Arabidopsis thaliana, a member of the AS2/LOB family, is involved development symmetrical expanded lamina, with rachis as axis. On flowering, transcripts AS2 were detected inflorescence meristems, floral and primordia all organs. Levels accumulation then decreased late stage organ primordia. β-glucuronidase (GUS) activities examined transgenic plants that had been transformed GUS controlled by promoter early stages flower development. We quantified length...
Arabidopsis ASYMMETRIC LEAVES2 (AS2) plays a key role in the formation of flat symmetric leaves. AS2 represses expression abaxial gene ETTIN/AUXIN RESPONSE FACTOR3 (ETT/ARF3). interacts vitro with CGCCGC sequence ETT/ARF3 exon 1. In cells leaf primordia, localizes at peripheral regions nucleolus as two bodies, which are partially overlapped chromocenters that contain condensed 45S ribosomal DNA repeats. contains AS2/LOB domain, consists three sequences conserved family: zinc finger (ZF)...
Somatic polyploidization often increases cell and organ size, thereby contributing to plant biomass production. However, as most woody plants do not undergo polyploidization, explaining the effect on growth in trees remains difficult. Here we developed a new method generate tetraploid lines poplars through colchicine treatment of lateral buds. We found that tetraploidization induced enlargement stem, suggesting can increase size cannot induce normal development. Greenhouse analysis revealed...