A5016112437- Plant-Microbe Interactions and Immunity
- HIV/AIDS drug development and treatment
- Protein Degradation and Inhibitors
- Multiple Myeloma Research and Treatments
- Plant Molecular Biology Research
- Plant Pathogenic Bacteria Studies
- Microbial Natural Products and Biosynthesis
- Chemical Synthesis and Analysis
- Legume Nitrogen Fixing Symbiosis
- HIV Research and Treatment
- Plant Parasitism and Resistance
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Biotin and Related Studies
- Plant Gene Expression Analysis
- Chromosomal and Genetic Variations
- Microtubule and mitosis dynamics
- Fungal and yeast genetics research
- Synthetic Organic Chemistry Methods
- Plant Disease Resistance and Genetics
- Click Chemistry and Applications
- Plant Reproductive Biology
- Plant Micronutrient Interactions and Effects
- DNA Repair Mechanisms
Max Planck Institute for Plant Breeding Research
2019-2025
Max Planck Society
2021-2023
Leiden University
2016-2021
Netherlands Metabolomics Centre
2017
University of Duisburg-Essen
2010-2013
Essen University Hospital
2013
Institut für Medizinische Informatik, Biometrie und Epidemiologie
2013
Chemical Genomics Centre
2010
Plants grown in natural soil are colonized by phylogenetically structured communities of microbes known as the microbiota. Individual can activate microbe-associated molecular pattern (MAMP)-triggered immunity (MTI), which limits pathogen proliferation but curtails plant growth, a phenomenon growth-defence trade-off. Here, we report that, monoassociations, 41% (62 out 151) taxonomically diverse root bacterial commensals suppress Arabidopsis thaliana growth inhibition (RGI) triggered...
Reactive oxygen species (ROS) are important messengers in eukaryotic organisms, and their production is tightly controlled. Active extracellular ROS by NADPH oxidases plants triggered receptor-like protein kinase-dependent signaling networks. Here, we show that CYSTEINE-RICH RLK2 (CRK2) kinase activity required for plant growth CRK2 exists a preformed complex with the oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) Arabidopsis (Arabidopsis thaliana). Functional full elicitor-induced...
Plants utilise intracellular nucleotide-binding, leucine-rich repeat (NLR) immune receptors to detect pathogen effectors and activate local systemic defence. NRG1 ADR1 "helper" NLRs (RNLs) cooperate with enhanced disease susceptibility 1 (EDS1), senescence-associated gene 101 (SAG101) phytoalexin-deficient 4 (PAD4) lipase-like proteins mediate signalling from TIR domain NLR (TNLs). The mechanism of RNL/EDS1 family protein cooperation is not understood. Here, we present genetic molecular...
Proteasome inhibitor (PI) carfilzomib (CFZ) has activity superior to bortezomib (BTZ) and is increasingly incorporated in multiple myeloma (MM) frontline therapy relapsed settings. Most MM patients ultimately experience PI-refractory disease, an unmet medical need with poorly understood biology dismal outcome. Pharmacologic targeting of ABCB1 improved patient outcomes, including MM, but suffered from adverse drug effects insufficient plasma concentrations. Proteomics analysis identified...
Chemical tools and methods that report on G protein-coupled receptor (GPCR) expression levels occupancy by small molecules are highly desirable. We the development of LEI121 as a photoreactive probe to study type 2 cannabinoid (CB2R), promising GPCR treat tissue injury inflammatory diseases. is first CB2R-selective bifunctional covalently captures CB2R upon photoactivation. An incorporated alkyne serves ligation handle for introduction reporter groups. enables target engagement studies...
Pattern-recognition receptor (PRR)-triggered immunity (PTI) wards off a wide range of pathogenic microbes, playing pivotal role in angiosperms. The model liverwort Marchantia polymorpha triggers defense-related gene expression upon sensing components bacterial and fungal extracts, suggesting the existence PTI this plant model. However, molecular putative M. significance bryophytes have not yet been described. We here show that has four lysin motif (LysM)-domain-containing homologs, two...
Abstract Mitosis and meiosis both rely on cohesin, which embraces the sister chromatids plays a crucial role for faithful distribution of chromosomes to daughter cells. Prior cleavage by Separase at anaphase onset, cohesin is largely removed from non-proteolytic action WINGS APART-LIKE (WAPL), mechanism referred as prophase pathway. To prevent premature loss chromatid cohesion, WAPL inhibited in early mitosis Sororin. However, Sororin homologs have only been found function inhibitors during...
Article26 September 2019Open Access Source DataTransparent process The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis Chao Yang Department of Developmental Biology, University Hamburg, Germany Search for more papers by this author Kostika Sofroni orcid.org/0000-0001-8648-4648 Erik Wijnker Yuki Hamamura Lena Carstens Hirofumi Harashima RIKEN Center Sustainable Resource Science, Yokohama, Japan Sara Christina Stolze Max-Planck-Institute Plant...
Arabidopsis pathogen effector-triggered immunity (ETI) is controlled by a family of three lipase-like proteins (EDS1, PAD4, and SAG101) two subfamilies HET-S/LOB-B (HeLo)-domain "helper" nucleotide-binding/leucine-rich repeats (ADR1s NRG1s). EDS1-PAD4 dimers cooperate with ADR1s, EDS1-SAG101 NRG1s, in separate defense-promoting modules. EDS1-PAD4-ADR1 EDS1-SAG101-NRG1 complexes were detected immune-activated leaf extracts but the molecular determinants for specific complex formation function...
Lipids play crucial roles in plant-microbe interactions, functioning as structural components, signaling molecules, and microbe-associated molecular patterns (MAMPs). However, the mechanisms underlying lipid perception plants remain largely unknown. Here, we investigate immune responses activated barley (Hordeum vulgare) by extracts from beneficial root endophytic fungus Serendipita indica compare them to elicited chitohexaose fungal sterol ergosterol. We demonstrate that S. extract induces...
Nutrient availability, in particular the availability of sugar (carbon (C)) and nitrogen (N), is important for regulation plant metabolism development. In addition to independent utilization C N nutrients, plants sense respond balance nutrients (C/N-nutrient) available them. High C/low N-nutrient stress has been shown arrest early post-germinative growth while promoting progression senescence Arabidopsis. Although several signaling components C/N-nutrient response have identified, inclusive...
Peptidic natural products are commonly perceived as 'simple' molecules that easily accessible by standard methods. This review sets out to demonstrate the total synthesis of peptidic is far from being 'standard' and highly elaborate methodologies paired with careful planning required attain their often complex structures. 1 Introduction 2 Peptide Synthesis Amino Acids Incompatible 'Standard' Conditions: The Feglymycin Tubulysin D 3 Macrocyclizations Pitfalls in Cyclo(depsi)peptides:...
Abstract Xylem vessels function in the long-distance conduction of water land plants. The NAC transcription factor VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a master regulator xylem vessel cell differentiation Arabidopsis (Arabidopsis thaliana). We previously isolated suppressor ectopic induced by VND7 (seiv) mutants. Here, we report that responsible genes for seiv3, seiv4, seiv6, and seiv9 are protein ubiquitination-related encoding PLANT U-BOX46 (PUB46), an uncharacterized F-BOX (FBX), PUB36,...
Abstract To optimize growth and development, plants monitor photosynthetic activities appropriately regulate various cellular processes. However, signaling mechanisms that coordinate plant with photosynthesis remain poorly understood. identify factors are involved in related to stimuli, we performed a phosphoproteomic analysis Marchantia polymorpha, an extant bryophyte species the basal lineage of land plants. Among proteins whose phosphorylation status changed differentially between...
Summary Ustilago maydis is a biotrophic fungus that causes tumor formation on all aerial parts of maize. U. secretes effector proteins during penetration and colonization to successfully overcome the plant immune response reprogram host physiology promote infection. In this study, we functionally characterized protein Topless (TPL) interacting 6 (Tip6). We found Tip6 interacts with N‐terminus RELK2 through its two Ethylene‐responsive element binding factor‐associated amphiphilic repression...
Several pathways conferring environmental flowering responses in Arabidopsis (Arabidopsis thaliana) converge on developmental processes that mediate the floral transition shoot apical meristem. Many characterized mutations disrupt these responses, but downstream have been more refractory to mutagenesis. Here, we constructed a quintuple mutant impaired several and showed it possesses severely reduced changes photoperiod ambient temperature. RNA-sequencing (RNA-seq) analysis of expression...
The solid phase total synthesis of the marine cyanobacterial Ahp-cyclodepsipeptide Symplocamide A is reported as a model for general route tailor-made non-covalent serine protease inhibitors.
Arabis alpina is a polycarpic perennial, in which PERPETUAL FLOWERING1 (PEP1) regulates flowering and perennial traits vernalization-dependent manner. Mutagenesis screens of the pep1 mutant established role other time regulators PEP1-parallel pathways. Here we characterized three allelic enhancers (eop002, 085 091) flower early. We mapped causal mutations complemented mutants with identified gene. Using quantitative reverse transcriptase PCR reporter lines, determined protein spatiotemporal...