A5027722849- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
- Biotin and Related Studies
- Microbial Natural Products and Biosynthesis
- Plant tissue culture and regeneration
- Immune Response and Inflammation
- Plant Molecular Biology Research
- Cellular transport and secretion
- Marine Toxins and Detection Methods
- Epigenetics and DNA Methylation
- Diatoms and Algae Research
- Plant Taxonomy and Phylogenetics
- Photoreceptor and optogenetics research
- Plant Diversity and Evolution
- Marine and coastal ecosystems
- Mycorrhizal Fungi and Plant Interactions
- Protist diversity and phylogeny
- Plant Reproductive Biology
- Invertebrate Immune Response Mechanisms
- Bacteriophages and microbial interactions
- Advanced Proteomics Techniques and Applications
- Microbial Community Ecology and Physiology
- Transgenic Plants and Applications
- Natural product bioactivities and synthesis
Laboratoire de Recherche en Sciences Végétales
2023-2024
Max Planck Institute for Plant Breeding Research
2021-2024
Université Toulouse III - Paul Sabatier
2024
Université de Toulouse
2024
Centre National de la Recherche Scientifique
2024
Institut National Polytechnique de Toulouse
2024
Carl von Ossietzky Universität Oldenburg
2018
Pattern-recognition receptor (PRR)-triggered immunity (PTI) wards off a wide range of pathogenic microbes, playing pivotal role in angiosperms. The model liverwort Marchantia polymorpha triggers defense-related gene expression upon sensing components bacterial and fungal extracts, suggesting the existence PTI this plant model. However, molecular putative M. significance bryophytes have not yet been described. We here show that has four lysin motif (LysM)-domain-containing homologs, two...
Toll/Interleukin-1 receptor (TIR) domains are integral to immune systems across all kingdoms. In plants, TIRs present in nucleotide-binding leucine-rich repeat (NLR) receptors, NLR-like, and TIR-only proteins. Although TIR-NLR TIR signaling plants require the ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) protein family, persist species that have no EDS1 members. To assess whether particular groups evolved with EDS1, we searched for TIR-EDS1 co-occurrence patterns. Using a large-scale phylogenetic...
Investigation of plant-bacteria interactions requires quantification in planta bacterial titers by means cumbersome and time-consuming colony-counting assays. Here, we devised a broadly applicable tool for bioluminescence-based quantitative spatial detection bacteria plants. We developed vectors that enable Tn7 transposon-mediated integration the luxCDABE luciferase operon into specific genomic location found ubiquitously across phyla. These allowed generation bioluminescent transformants...
Agrobacterium-mediated transient gene expression is a rapid and useful approach for characterizing functions of products in planta. However, the practicability method model liverwort Marchantia polymorpha has not yet been thoroughly described. Here we report simple robust transformation thalli its applicability. When M. were co-cultured with Agrobacterium tumefaciens carrying β-glucuronidase (GUS) genes, GUS staining was observed primarily assimilatory filaments rhizoids. activity detected 2...
Abstract Toll/interleukin-1 Receptor (TIR) domains are integral to immune systems across all of life. TIRs exist as single-domain and larger receptor or adaptor proteins. In plants, constitute N-terminal nucleotide-binding leucine-rich repeat (NLR) receptors. Although TIR-NLR TIR signaling requires the Enhanced disease susceptibility 1 (EDS1) protein family, persist in species that have incomplete no EDS1 members. To assess whether particular groups appear with EDS1, we searched for TIR-EDS1...
Abstract The sea-surface microlayer (SML) at the air-sea interface is a distinct, under-studied habitat compared to subsurface and copepods, important components of ocean food webs, have developed key adaptations exploit this niche. By using automated SML sampling, high-throughput sequencing unmanned aerial vehicles, we report on distribution abundance pontellid copepods in relation unique biophysicochemical signature SML. We found even during high exposure sun-derived ultraviolet radiation...
Marchantia polymorpha is a model liverwort and its overall low genetic redundancy advantageous for dissecting complex pathways. Proximity-dependent in vivo biotin-labelling methods have emerged as powerful interactomics tools recent years. However, studies applying proximity labelling are currently limited to angiosperm species plants. Here, we established evaluated miniTurbo-based method M. using MpSYP12A MpSYP13B, two plasma membrane-localized SNARE proteins, baits. We show that our yields...
SUMMARY Investigation of plant-bacteria interactions requires quantification in planta bacterial titers by means colony counting assays. However, assays are cumbersome and time-consuming, unable to detect spatial patterns colonization plants. Here, overcome these shortcomings, we devised a broadly applicable genetic engineering tool for bioluminescence-based quantitative detection bacteria We developed plasmid vectors that have broad host ranges enable Tn 7 transposon-mediated integration...
Several excursions have been undertaken to sample marine, freshwater and terrestrial habitats for the presence of microscopic algae on around Canary Islands. Sampling isolation efforts resulted in several newly established cryptophyte cultures. To preliminarily determine their identity, nuclear SSU rDNA was sequenced subjected molecular phylogenetic analyses. All cultures belong currently known major clades, five out six lineages likely represent new Cryptomonas species.
Summary SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASES (SERKs), which are subfamily II of leucine-rich repeat receptor-like kinases (LRR-RLKs), play diverse roles in development and immunity the angiosperm Arabidopsis thaliana . AtSERKs act as co-receptors for many LRR-RLKs, including BRASSINOSTEROID INSENSITIVE 1 (BRI1) FLAGELLIN SENSITIVE 2 (FLS2). 1–4 The conserved tyrosine (Y) residue AtSERK3 is crucial signaling specificity differentiating BRI1- FLS2-mediated pathways. 5 BRI1-ASSOCIATED...
Abstract Pattern-recognition receptor (PRR)-triggered immunity (PTI) wards off a wide range of pathogenic microbes, playing pivotal role in plant immunity. The model liverwort Marchantia polymorpha is emerging as popular for investigating the evolution plant-microbe interactions. M. triggers defense-related gene expression upon sensing components bacterial and fungal extracts, suggesting existence PTI this model. However, molecular putative have not yet been described. We show that, , which...
Abstract Agrobacterium -mediated transient gene expression is a rapid and useful approach for characterizing functions of products in planta . However, the practicability method model liverwort Marchantia polymorpha has not yet been thoroughly described. Here we report simple robust transformation thalli its applicability. When M. were co-cultured with tumefaciens carrying GUS genes, staining was observed primarily assimilatory filaments rhizoids. activity detected 2 days after infection...
Summary Marchantia polymorpha is a model liverwort and its overall low genetic redundancy advantageous for dissecting complex pathways. Proximity-dependent in vivo biotin-labelling methods have emerged as powerful interactomics tools recent years. However, studies applying proximity labelling are currently limited to angiosperm species plants. Here, we established evaluated miniTurbo-based method M. using MpSYP12A MpSYP13B, two plasma membrane- localized SNARE proteins, baits. We show that...