A5017042035- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Bryophyte Studies and Records
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- ATP Synthase and ATPases Research
- Cellular transport and secretion
- Plant and animal studies
- Metabolism and Genetic Disorders
- Plant-Microbe Interactions and Immunity
- Adrenal and Paraganglionic Tumors
- Phagocytosis and Immune Regulation
- Sodium Intake and Health
- Metalloenzymes and iron-sulfur proteins
- Eicosanoids and Hypertension Pharmacology
- Islanding Detection in Power Systems
- Microbial metabolism and enzyme function
- Pineapple and bromelain studies
- Organic Chemistry Synthesis Methods
- Plant Diversity and Evolution
- Pharmacological Receptor Mechanisms and Effects
- Food Quality and Safety Studies
- Peatlands and Wetlands Ecology
National Institute for Basic Biology
2017-2025
The Graduate University for Advanced Studies, SOKENDAI
2017-2023
Nippon Soken (Japan)
2020
The University of Tokyo
2013-2017
Chiba University
2012-2013
Hokkaido University
2008
Hirosaki University
1988
The evolution of land flora transformed the terrestrial environment. Land plants evolved from an ancestral charophycean alga which they inherited developmental, biochemical, and cell biological attributes. Additional biochemical physiological adaptations to land, a life cycle with alternation between multicellular haploid diploid generations that facilitated efficient dispersal desiccation tolerant spores, in plant. We analyzed genome liverwort Marchantia polymorpha, member basal plant...
Abstract The liverwort Marchantia polymorpha has been utilized as a model for biological studies since the 18th century. In past few decades, there Renaissance in its utilization genomic and genetic approaches to investigating physiological, developmental, evolutionary aspects of land plant biology. reasons adoption are similar those other models, e.g. simple cultivation, ready access via worldwide distribution, ease crossing, facile genetics, more recently, efficient transformation, genome...
Pattern-recognition receptor (PRR)-triggered immunity (PTI) wards off a wide range of pathogenic microbes, playing pivotal role in angiosperms. The model liverwort Marchantia polymorpha triggers defense-related gene expression upon sensing components bacterial and fungal extracts, suggesting the existence PTI this plant model. However, molecular putative M. significance bryophytes have not yet been described. We here show that has four lysin motif (LysM)-domain-containing homologs, two...
The membrane trafficking pathway has been diversified in a specific way for each eukaryotic lineage, probably to fulfill functions the organisms. In green plants, comparative genomics supported possibility that terrestrialization and/or multicellularization could be associated with elaboration and diversification of pathways, which have accomplished by an expansion numbers genes required machinery components trafficking, including soluble N -ethylmaleimide-sensitive factor attachment protein...
Abstract Eukaryotic cells acquired novel organelles during evolution through mechanisms that remain largely obscure. The existence of the unique oil body compartment is a synapomorphy liverworts represents lineage-specific acquisition this organelle evolution, although its origin, biogenesis, and physiological function are yet unknown. We find two paralogous syntaxin-1 homologs in liverwort Marchantia polymorpha distinctly targeted to forming cell plates body, suggesting these structures...
Summary Each eukaryotic cell possesses a specialized membrane trafficking system that emerged through paralogous expansion followed by the neofunctionalization of machinery components, including SNARE proteins, during evolution. We discovered acquisition an acidic insertion in polypeptide converted secretory R-SNARE VAMP72 into major component plant vacuolar transport. The moderately insertion, originating from alternative splicing ancestral embryophyte, conferred binding ability to clathrin...
The V-ATPase is a versatile proton-pump found in range of endomembrane compartments yet the mechanisms governing its differential targeting remain to be determined. In Arabidopsis, VHA-a1 targets TGN/EE whereas VHA-a2 and VHA-a3 are localized tonoplast. We report here that domain serves as both an ER-exit TGN/EE-retention motif conserved among seed plants. contrast, Marchantia encodes single VHA-isoform localizes tonoplast Arabidopsis. Analysis CRISPR/Cas9 generated null alleles revealed has...
The plastids of plant cells each contain their own genome, and a bacterial-type RNA polymerase called plastid-encoded plastid (PEP) is involved in transcription this genome. While the catalytic core subunits are encoded by specificity subunit PEP, sigma, generally nuclear genome imported into from cytoplasm after translation. In study, we identified analyzed four sigma factor genes liverwort, Marchantia polymorpha. Phylogenetic analysis suggested that three were orthologous to vascular thus...
Autophagy is a catabolic process for bulk and selective degradation of cytoplasmic components in the vacuole/lysosome. In Saccharomyces cerevisiae, ATG genes were identified as essential autophagy, most are highly conserved among eukaryotes, including plants. Although reverse genetic analyses have revealed that autophagy involved responses to abiotic biotic stresses land plants, our knowledge its molecular mechanism remains limited. This limitation partly because multiplication some genes,...
The RAB GTPase is an evolutionarily conserved machinery component of membrane trafficking, which the fundamental system for cell viability and higher order biological functions. composition GTPases in each organism closely related to complexity organization trafficking pathway, has been developed uniquely realize organism-specific system. Comparative genomics suggested that terrestrialization and/or multicellularization were associated with expansion pathways green plants, yet be validated...
Hepatocyte nuclear factor (HNF) 4alpha, a transcription of the hormone receptor family, is generally expressed in some endoderm-derived epithelial tissues such as hepatocytes. In mice, an alternative promoter referred to P2 located upstream from P1 promoter, resulting at least nine isoforms. this study, we investigated expression Hnf4alpha adult and embryonic mouse tissues, paying special attention developing metanephros by using immunohistochemistry reverse transcriptase-polymerase chain...
Eukaryotic cells possess endomembrane organelles equipped with specific sets of proteins, lipids, and polysaccharides that are fundamental for realizing each organelle’s function shape. A tightly regulated membrane trafficking system mediates the transportation localization these substances. Generally, secretory/exocytic pathway is responsible transporting cargo to plasma and/or extracellular space. However, in case oil body liverwort Marchantia polymorpha, body, a liverwort-unique...
Previous studies have revealed duplications and diversification of myosin XI genes between angiosperms bryophytes; however, the functional differentiation conservation them remain unclear. Here, we identified a single gene from liverwort Marchantia polymorpha (Mp). The molecular properties Mp are similar to those Arabidopsis XIs responsible for cytoplasmic streaming, suggesting that motor function is able generate streaming. In cultured cells, transiently expressed green fluorescent protein...
We investigated the correlation between aging and sensitivity of blood pressure to salt. 88 non-treated essential hypertensives were divided into four groups: less than 40 years old (n = 20), 40-49 50-59 39), greater 60 11). Changes pressure, plasma renin activity (PRA), aldosterone concentration (PAC), norepinephrine (PNE), epinephrine (PE) due salt load compared among groups. Salt was increased with aging, there a positive them (r 0.30, p < 0.01). Decrement PRA decreased negative -0.35,...
<title>Abstract</title> The distinctive protein and lipid composition underlies the distinct function of each organelle, regulated by balanced anterograde retrograde membrane trafficking. vacuole, largest plant is pivotal in various functions, with its tightly bidirectional However, existence transport from vacuole remained unverified. In this study, we demonstrate trafficking Arabidopsis. We observed retrieval VAMP727, a plant-unique vacuolar fusion machinery, membrane. VAMP727 facilitated...
Cells of land plants contain several kinds plastids such as chloroplasts, etioplasts, proplastids, leucoplasts, and amyloplasts. Among them, the chloroplast, most well characterized type plastid, relies on expression plastid-encoded photosynthetic genes. The genome encodes many genes that are mainly transcribed by plastid RNA polymerase (PEP). Transcriptional activity PEP is controlled nuclear-encoded sigma factors important for transcription initiation promoter selectivity. Arabidopsis...
Abstract Eukaryotic cells acquired novel organelles during evolution through mechanisms that remain largely obscure. The existence of the unique oil body compartment is a synapomorphy liverworts represents lineage-specific acquisition this organelle evolution, although its origin, biogenesis, and physiological function are yet unknown. We found two Syntaxin 1 paralogs in liverwort, Marchantia polymorpha , distinctly targeted to forming cell plates body, suggesting these structures share some...