A5018183554- Lipid metabolism and biosynthesis
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Plant biochemistry and biosynthesis
- Plant Parasitism and Resistance
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Natural product bioactivities and synthesis
- Plant Gene Expression Analysis
- Plant Virus Research Studies
- Botany and Plant Ecology Studies
- Plant Micronutrient Interactions and Effects
- Plant-Microbe Interactions and Immunity
- Bryophyte Studies and Records
- Genomics and Phylogenetic Studies
- Plant Stress Responses and Tolerance
- Biocrusts and Microbial Ecology
- Radioactive contamination and transfer
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Aluminum toxicity and tolerance in plants and animals
- Kruppel-like factors research
- Plant Disease Resistance and Genetics
Kyoto University
2011-2022
Kindai University
2019-2022
The University of Tokyo
2011-2018
Nara Institute of Science and Technology
2008-2017
The advent of image-activated cell sorting and imaging-based picking has advanced our knowledge exploitation biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure molecular landscape cell, which critical limitations. Here we demonstrate Raman by directly probing chemically specific intracellular vibrations via ultrafast multicolor stimulated scattering (SRS) microscopy phenotyping. Specifically,...
Abstract Tobacco (Nicotiana tabacum) synthesizes nicotine and related pyridine alkaloids in the root, their synthesis increases upon herbivory on leaf via a jasmonate-mediated signaling cascade. Regulatory NIC loci that positively regulate biosynthesis have been genetically identified, mutant alleles used to breed low-nicotine tobacco varieties. Here, we report NIC2 locus, originally called locus B, comprises clustered transcription factor genes of an ethylene response (ERF) subfamily; nic2...
In tobacco (Nicotiana tabacum), nicotine is the predominant alkaloid. It produced in roots and accumulated mainly leaves. Jasmonates play a central signaling role damage-induced formation. The genome sequence of provides us an almost complete inventory structural regulatory genes involved pathway. Phylogenetic expression analyses revealed series pathway, forming regulon, under control jasmonate-responsive ETHYLENE RESPONSE FACTOR (ERF) transcription factors. duplication NAD polyamine...
Y chromosomes are different from other because of a lack recombination. Until now, complete sequence information has been available only for some primates, although considerable is organisms, e.g., several species Drosophila. Here, we report the gene organization chromosome in dioecious liverwort Marchantia polymorpha and provide detailed view haploid organism. On 10-Mb chromosome, 64 genes identified, 14 which detected male genome expressed reproductive organs but not vegetative thalli,...
Abstract Tobacco (Nicotiana tabacum) plants synthesize nicotine and related pyridine-type alkaloids, such as anatabine, in their roots accumulate them aerial parts chemical defenses against herbivores. Herbivory-induced jasmonate signaling activates structural genes for biosynthesis transport by way of the NICOTINE (NIC) regulatory loci. The tobacco alkaloids involves condensation an unidentified nicotinic acid-derived metabolite with N-methylpyrrolinium cation or itself, but exact enzymatic...
Although microalgae accumulate triacylglycerol (TAG) and starch in response to nutrient-deficient conditions, the regulatory mechanisms are poorly understood. We report here identification characterization of a kinase, accumulation regulator1 (TAR1), that is member yeast (Saccharomyces cerevisiae) Yet another kinase1 (Yak1) subfamily dual-specificity tyrosine phosphorylation-regulated kinase family green alga (Chlamydomonas reinhardtii). The domain TAR1 showed auto- transphosphorylation...
Several microalgae accumulate high levels of squalene, and as such provide a potentially valuable source this useful compound. However, the molecular mechanism squalene biosynthesis in is still largely unknown. We obtained sequences two enzymes involved synthesis metabolism, synthase (CrSQS) epoxidase (CrSQE), from model green alga Chlamydomonas reinhardtii. CrSQS was functionally characterized by expression Escherichia coli CrSQE complementation budding yeast erg1 mutant. Transient fused...
Arabidopsis thaliana BOR1 was the first boron (B) transporter identified in living systems. There are four AtBOR1-like genes, OsBOR1, 2, 3 and 4, present rice genome. We characterized activity, expression physiological function of OsBOR4. OsBOR4 is an active efflux B. Quantitative PCR analysis promoter–green fluorescent protein (GFP) fusion revealed that both highly specifically expressed pollen. obtained five Tos17 insertion mutants osbor4. The pollen grains were viable development floral...
Autophagy is a recycling system for amino acids and carbon- nitrogen (N)-containing compounds. To date, the functional importance of autophagy in microalgae nutrient-deficient conditions has not been evaluated by using autophagy-defective mutants. Here, we provide evidence which supports following notions characterizing an insertional mutant autophagy-related gene ATG8, encoding ubiquitin-like protein necessary formation autophagosome green alga, Chlamydomonas reinhardtii. First, ATG8...
Endoplasmic reticulum (ER) stress is caused by the stress-induced accumulation of unfolded proteins in ER. Here, we identified and lipids that function downstream ER sensor INOSITOL-REQUIRING ENZYME1 (CrIRE1) contributes to tolerance Chlamydomonas (Chlamydomonas reinhardtii). Treatment with inducer tunicamycin resulted splicing a 32-nucleotide fragment basic leucine zipper 1 (bZIP1) transcription factor (CrbZIP1) mRNA CrIRE1 that, turn, loss transmembrane domain CrbZIP1, translocation...
The elucidation of lipid metabolism in microalgae has attracted broad interest, as their storage lipid, triacylglycerol (TAG), can be readily converted into biofuel via transesterification. TAG accumulates the form oil droplets, especially when cells undergo nutrient deprivation, such for nitrogen (N), phosphorus (P), or sulfur (S). biosynthesis under N-deprivation been comprehensively studied model microalga Chlamydomonas reinhardtii, during which dramatically. However, resulting rapid...
Abstract Photosynthetic organisms are exposed to various environmental sources of oxidative stress. Land plants have diverse mechanisms withstand stress, but how microalgae do so remains unclear. Here, we characterized the Chlamydomonas reinhardtii basic leucine zipper (bZIP) transcription factor BLZ8, which is highly induced by Oxidative stress tolerance increased with increasing BLZ8 expression levels. regulated genes likely involved in carbon-concentrating mechanism (CCM): HIGH-LIGHT...
The liverwort Marchantia polymorpha L. synthesizes arachidonic (ARA) and eicosapentaenoic acids (EPA) from linoleic α-linolenic respectively by a series of reactions catalyzed Δ6-desaturase, Δ6-elongase, Δ5-desaturase. Overexpression the M. genes encoding these enzymes in transgenic plants resulted 3- 2-fold accumulation ARA EPA respectively, as compared to those wild type. When three were introduced co-expressed tobacco plants, which long-chain polyunsaturated fatty (LCPUFAs) are not native...
Pinolenic acid (PA; 18:3Delta(5,9,12)) and coniferonic (CA; 18:4Delta(5,9,12,15)) are Delta(5)-unsaturated bis-methylene-interrupted fatty acids (Delta(5)-UBIFAs) commonly found in pine seed oil. They assumed to be synthesized from linoleic (LA; 18:2Delta(9,12)) alpha-linolenic (ALA; 18:3Delta(9,12,15)), respectively, by Delta(5)-desaturation. A unicellular green microalga Chlamydomonas reinhardtii also accumulates PA CA a betain lipid. The expressed sequence tag (EST) resource of C. led the...
CtBP/BARS is a unique protein family in having quite diversified cellular functions, intercellular localizations, and developmental roles. ANGUSTIFOLIA (AN) the sole homolog of from Arabidopsis thaliana, although it has plant AN-specific motifs long C-terminus. Previous studies suggested that AN would function nucleus as transcriptional co-repressor, CtBPs animals; however, precise verification been lacking. In this paper, we isolated homologous gene (MAN) liverwort, Marchantia polymorpha....