A5046288190- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Aluminum toxicity and tolerance in plants and animals
- RNA modifications and cancer
- Silicon Effects in Agriculture
- Plant Genetic and Mutation Studies
- Plant Reproductive Biology
- Nitrogen and Sulfur Effects on Brassica
- Polymer-Based Agricultural Enhancements
- Legume Nitrogen Fixing Symbiosis
- Microfluidic and Bio-sensing Technologies
- Remote Sensing in Agriculture
- Selenium in Biological Systems
- Plant tissue culture and regeneration
- Agriculture, Soil, Plant Science
- DNA Repair Mechanisms
- Plant and animal studies
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Hydrogels: synthesis, properties, applications
- GABA and Rice Research
The University of Tokyo
2016-2025
Cardiff University
2021-2025
Tokyo Metropolitan University
2024-2025
Osaka Metropolitan University
2024
Tokyo University of Science
2021
Graduate School USA
2021
Upstream open reading frames (uORFs) are often translated ahead of the main ORF a gene and regulate expression, sometimes in condition-dependent manner, but such role for minimum uORF (hereafter referred to as AUG-stop) living organisms is currently unclear. Here, we show that AUG-stop plays an important boron (B)-dependent regulation NIP5;1, encoding boric acid channel required normal growth under low B conditions Arabidopsis thaliana. High enhanced ribosome stalling at AUG-stop, which was...
Specialized (secondary) metabolic pathways in plants have long been considered one-way routes of leading primary metabolite precursors to bioactive end products. Conversely, endogenous degradation such “end” products plant tissues has observed following environmental stimuli, including nutrition stress. Therefore, it is general interest whether specialized metabolites can be reintegrated into metabolism recover the invested resources, especially case nitrogen- or sulfur-rich compounds. Here,...
Abstract Legumes control root nodule symbiosis (RNS) in response to environmental nitrogen availability. Despite the recent understanding of molecular basis external nitrate-mediated RNS, it remains mostly elusive how plants regulate physiological processes depending on internal status. In addition, iron (Fe) acts as an essential element that enables symbiotic fixation; however, mechanism Fe accumulation nodules is poorly understood. Here, we focus transcriptome status during RNS Lotus...
High levels of boron (B) induce DNA double-strand breaks (DSBs) in eukaryotes, including plants. Here we show a molecular pathway high B-induced DSBs by characterizing Arabidopsis thaliana hypersensitive to excess mutants. Molecular analysis the mutants revealed that degradation SWItch/Sucrose Non-Fermentable subunit, BRAHMA (BRM), 26S proteasome (26SP) with specific subunits is key process for ameliorating high-B-induced DSBs. We also found high-B treatment induces histone hyperacetylation,...
Observation of plant root morphology in soil is fundamental importance research, but the lack transparency hampers direct observation roots. One approaches to overcome this technical limitation use "transparent soil" (TS), hydrogel-based beads produced by spherification gelling agents. However, production TS natural dripping solution can be labor intensive, time consuming and difficult maintain consistent product quality. Here we present a semi-automated system for production. A...
In eukaryotes, messenger RNA (mRNA) accumulation is regulated through the levels of transcription, processing, and degradation. Here, we uncover multi-level regulatory mechanism governing expression NIP5;1, a boron (B) diffusion facilitator in Arabidopsis. B-dependent NIP5;1 mRNA degradation triggered by ribosome stalling at an AUGUAA sequence its 5'-untranslated region. We showed that deletion ATGTAA also abolishes transcriptional downregulation, revealing dual role this both control. Small...
Boron (B) is an essential micronutrient for plants. Efflux-type B transporters, BORs, have been identified in Arabidopsis thaliana and rice. Here we BOR1 genes encoding efflux from the hexaploid genome of wheat (Triticum aestivum L.). We cloned three closely related to OsBOR1 named them TaBOR1.1, TaBOR1.2 TaBOR1.3. All TaBOR1s showed activities when expressed tobacco BY-2 cells. TaBOR1-green fluorescent protein (GFP) fusion proteins were leaf cells localized plasma membrane. The transcript...
Boron, an essential micronutrient, is transported in roots of Arabidopsis thaliana mainly by two different types transporters, BORs and NIPs (nodulin26-like intrinsic proteins). Both are plasma membrane localized, but have distinct transport properties patterns cell type-specific accumulation with polar localizations, which likely to affect boron distribution. Here, we used mathematical modeling experimental determination address distributions the root. A computational model root created at...
Recent years have seen the development of novel, rapid, and inexpensive techniques for collecting plant data to monitor nutritional status crops. These include hyperspectral imaging, which has been widely used in combination with machine learning models predict element concentrations plants. When there are multiple elements, trained spectral features individual concentrations; this type single-target prediction is known as regression. Although method can achieve reliable accuracy some others...
We previously reported that ribosome stalling at AUG-stop sequences in the 5'-untranslated region plays a critical role regulating expression of Arabidopsis thaliana NIP5;1, which encodes boron uptake transporter, response to conditions media. This is triggered specifically by boric acid, but mechanisms are unknown. Although upstream open reading frames (uORFs) known many cases regulate translation through peptides encoded uORF, does not involve any peptide synthesis. The unique feature...
Abstract Bioassay with an insect herbivore is a common approach to studying plant defense. While measuring growth rate as negative indicator of defense levels simple and straightforward, analysing more detailed feeding behavior parameters insects, such rates, leaf area consumed per event, intervals between events, spatio-temporal patterns sites on leaves, informative. However, observations are generally time consuming labor-intensive. Here, we provide semi-automated system for quantifying...
To increase our understanding of the adaptation for copper (Cu) deficiency, Arabidopsis mutants with apparent alterations under Cu deficiency were identified. In this report, a novel mutant, tpst-2, was found to be more sensitive than wild-type (Col-0) plants during root elongation. The positional cloning tpst-2 revealed that gene encodes tyrosylprotein sulfotransferase (TPST). Moreover, ethylene production mutant higher Col-0 and adding response inhibitor AgNO3 partially rescued defects in...
Nutrient uptake by roots often involves substrate-dependent regulated nutrient transporters. For robust uptake, the system requires a regulatory circuit within cells and collective, coordinated behaviour across tissue. A paradigm for such systems is boron known its directional transport homeostasis, as essential plant growth but toxic at high concentrations. In Arabidopsis thaliana, occurs via diffusion facilitators (NIPs) exporters (BORs), each presenting distinct polarity. Intriguingly,...
Boron (B), an essential micronutrient, causes adverse effects on the growth and development of plants when highly accumulated. By analysis Arabidopsis mutants hypersensitive to high-boron (high-B) stress, we have shown that 26S proteasome (26SP) is required maintain morphology root apical meristem (RAM) under high-B stress. To further understand molecular function 26SP in tolerance stress RAM, this study investigated pathways regulated by using a subunit mutant, rpt5a, which Expression RPT5a...
Excess boron (B) is toxic to plants and thereby causes DNA damage cell death in root meristems. However, the underlying mechanisms which link remain unclear. It has been reported that rpt5a-6 mutant of 26S proteasome sensitive excess boron, resulting more frequent meristem reduced elongation. In this study, we showed a reduction growth rpt5a presence high levels repressed by mutation NAC domain containing transcription factor NAC103, substrate proteasome, functions unfolded protein response...
Root growth in plants is achieved through the co-ordination of cell division and expansion. In higher plants, radial structure roots formed during embryogenesis maintained thereafter throughout development. Here we show that tetratricopeptide repeat domain protein TPR5 necessary for maintaining rates Arabidopsis thaliana roots. We isolated an A. mutant with reduced root determined was gene responsible phenotype. The rate tpr5-1 to ~60% wild-type plants. disturbed by occurrence occasional...
This protocol illustrates semi-automated production of hydrogel-based "transparent soil" for plant or bacterial research. It utilizes cassette tube pump to control liquid flow and water levels achieve stable spherification, resulting in higher yield with less labor.
Mg2+ is among the most abundant divalent cations in living cells. In plants, investigations on magnesium (Mg) homeostasis are restricted to functional characterization of transporters. Here, we demonstrate that splicing factors SUPPRESSORS OF MEC-8 AND UNC-52 1 (SMU1) and SMU2 mediate Mg Arabidopsis (Arabidopsis thaliana). A low-Mg sensitive mutant was isolated, causal gene identified as SMU1. Disruption SMU2, a protein can form complex with SMU1, resulted similar phenotype. both mutants, an...
Recent accumulation of genomic and transcriptomic information has facilitated genetic studies. Increasing evidence demonstrated that translation is an important regulatory step, the transcriptome does not necessarily reflect profile functional protein production. Deep sequencing ribosome-protected mRNA fragments (ribosome profiling or Ribo-seq) enabled genome-wide analysis translation. Sorghum a C4 cereal only as food but also forage bioenergy resource. Its resistance to harsh environments...
Abstract Leaf development in plants, including dorsoventral (adaxial–abaxial) patterning, is tightly regulated. The involvement of several subunits the 26S proteasome adaxial–abaxial polarity establishment has been reported. In present study, we revealed that Arabidopsis thaliana , a mutation RPT5A subunit proteasome, causes abnormally narrow true leaves under zinc deficiency. mRNA accumulations DNA damage marker genes were elevated by PARP2 single-strand break (SSB) inducible gene, was more...
Here, we describe a method for obtaining thin cross sections of Arabidopsis thaliana roots without fixation and embedding. Roots were grown in pinholes made solidified growth medium, prepared pretreatment. Using this method, detected unique distributions two polar-localized proteins—green fluorescent protein (GFP)-tagged BOR1 NIP5;1—with less sample preparation time than conventional methods. This is simple, rapid, yields high-quality cross-section images that are free from artifacts...
Mineral concentrations in cereals are crucial for human health, especially people who consume as a subsistence diet. Although rice (Oryza sativa L.) is one of most important staple crops the world, starch constitutes large portion its grains, and mineral lower than other crops. One solution improvement grains through biofortification, this approach requires investigation genetic resources that confer high to grains. In present study, we performed 2 years field experiments, 2012 2013,...