A5063736660- Plant Molecular Biology Research
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Plant Genetic and Mutation Studies
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Advanced Fluorescence Microscopy Techniques
- CRISPR and Genetic Engineering
- Nuclear Structure and Function
- DNA Repair Mechanisms
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- Advanced X-ray Imaging Techniques
- Plant and animal studies
- RNA and protein synthesis mechanisms
- Algal biology and biofuel production
- Microbial Community Ecology and Physiology
- Laser Material Processing Techniques
The University of Tokyo
2004-2025
Hitachi (Japan)
2024
Tokyo University of Science
2014-2023
Toyo University
2021
Japan Science and Technology Agency
2019
Romanian Institute of Science and Technology
2016-2017
Fracture Analysis Consultants (United States)
2016
Science and Technology Interactive Center
2016
Tottori University
2014
Osaka University
2003-2012
The whole genome of Jatropha curcas was sequenced, using a combination the conventional Sanger method and new-generation multiplex sequencing methods. Total length non-redundant sequences thus obtained 285 858 490 bp consisting 120 586 contigs 29 831 singlets. They accounted for ∼95% gene-containing regions with average G + C content 34.3%. A total 40 929 complete partial structures protein encoding genes have been deduced. Comparison other plant species indicated that 1529 (4%) putative...
Genome integrity is continuously threatened by external stresses and endogenous hazards such as DNA replication errors reactive oxygen species. The damage checkpoint in metazoans ensures genome delaying cell-cycle progression to repair damaged or inducing apoptosis. ATM ATR (ataxia-telangiectasia-mutated -Rad3-related) are sensor kinases that relay the signal transducer Chk1 Chk2 downstream regulators. Plants also possess orthologs but lack obvious counterparts of Instead, plant-specific...
Legumes and rhizobia establish symbiosis in root nodules. To balance the gains costs associated with symbiosis, plants have developed two strategies for adapting to nitrogen availability soil: can regulate nodule number and/or stop development or function of Although former is accounted by autoregulation nodulation, a form systemic long-range signaling, latter strategy remains largely enigmatic. Here, we show that Lotus japonicus NITRATE UNRESPONSIVE SYMBIOSIS 1 (NRSYM1) gene encoding...
Subcellular organelles in living cells were inactivated by tightly focusing femtosecond laser pulses inside the cells. Photodisruption of a mitochondrion was experimentally confirmed stacking three-dimensional confocal images and restaining organelles. The viability after irradiation ascertained impermeability propidium iodide as well presence cytoplasmic streaming.
Plant B-type cyclin genes are expressed specifically in late G2- and M-phases during the cell cycle. Their promoters contain a common cis-acting element, called MSA (M-specific activator) that is necessary sufficient for periodic promoter activation. This motif also present tobacco kinesin-like protein gene NACK1, which with timing similar to of genes. In this study, we show G2/M-phase-specific activation NACK1 regulated by suggesting defined set coregulated an MSA-mediated mechanism. search...
Abstract Cytokinesis in plants is achieved by the formation of cell plate. A pathway that includes mitogen-activated protein (MAP) kinase and MAP (MAPKK) plays a key role control plant cytokinesis. We show here kinase, MPK4, required for plate Arabidopsis thaliana. Single mutations MPK4 caused dwarfism characteristic defects cytokinesis, such as immature plates, which became much more prominent upon introduction mutation MKK6/ANQ, MAPKK into mpk4. MKK6/ANQ strongly activated protoplasts,...
Abstract Although excess boron (B) is known to negatively affect plant growth, its molecular mechanism of toxicity unknown. We previously isolated two Arabidopsis thaliana mutants, hypersensitive B (heb1-1 and heb2-1). In this study, we found that HEB1 HEB2 encode the CAP-G2 CAP-H2 subunits, respectively, condensin II protein complex, which functions in maintenance chromosome structure. Growth seedlings medium containing induced expression subunit genes. Simultaneous treatment with zeocin,...
Abstract Increased cellular ploidy is widespread during developmental processes of multicellular organisms, especially in plants. Elevated levels are typically achieved either by endoreplication or endomitosis, which often regarded as modified cell cycles that lack an M phase entirely partially. We identified GIGAS CELL1 (GIG1)/OMISSION OF SECOND DIVISION1 (OSD1) and established mutation this gene triggered ectopic endomitosis. On the other hand, it has been reported a paralog GIG1/OSD1,...
Plant roots consist of a meristematic zone mitotic cells and an elongation rapidly expanding cells, in which DNA replication often occurs without cell division, process known as endoreduplication. The duration the cycle replication, measured by 5-ethynyl-2′-deoxy-uridine (EdU) incorporation, differed between two regions (17 h zone, 30 zone). Two distinct subnuclear patterns EdU signals, whole speckled, marked nuclei undergoing at early late S phase, respectively. boundary region zones was...
Parasitic plants share a common anatomical feature, the haustorium. Haustoria enable both infection and nutrient transfer, which often leads to growth penalties for host yield reduction in crop species. also reciprocally transfer substances, such as RNA proteins, from parasite host, but biological relevance movement remains unknown. Here, we studied interspecies transport by using hemiparasitic plant
Clearing techniques eliminate factors that interfere with microscopic observation, including light scattering and absorption by pigments cytoplasmic components. The allow fluorescence-based detailed analyses of materials characterization the three-dimensional structure organs. We describe a simple rapid clearing imaging method, termed 'TOMEI' (Transparent plant Organ MEthod for Imaging), which enables observation intact This method involves reagent containing 2,2′-thiodiethanol....
External application of acetic acid has been recently reported to enhance the survival drought in plants such as Arabidopsis, rapeseed, maize, rice and wheat, but effects on increased tolerance woody a tropical crop "cassava" remain elusive. A molecular understanding acid-induced avoidance cassava will contribute development technology that can be used without resorting transgenic or advancements cultivation. In present study, morphological, physiological responses were analyzed after...
A complex structure, designated the chromosome periphery, surrounds each during mitosis. Although several proteins have been shown to localize their functions mitosis remain unclear. Here, we used a combination of high-resolution microscopy and RNA-interference-mediated depletion study nucleolin, nucleolar protein localized at in interphase During mitosis, nucleolin was peripheral region including vicinity outer kinetochore chromosomes. Staining with an antibody specific for phosphorylated...
DNA is packaged as chromatin in the interphase nucleus. During mitosis, fibers are highly condensed to form metaphase chromosomes, which ensure equal segregation of replicated chromosomal into daughter cells. Despite >1 century research on information regarding higher order structure chromosomes limited, and it still not clear proteins involved further folding fiber chromosomes. To obtain a global view proteins, we performed proteome analyses three types isolated human We first show results...
NPM (nucleophosmin; also known as B23) is an abundantly and ubiquitously expressed multifunctional nucleolar phosphoprotein, which involved in numerous cellular processes, including ribosome biogenesis, protein chaperoning centrosome duplication; however, the role of cell cycle still remains unknown. In present study, we show dynamic localization throughout HeLa cells. Using a combination RNAi (RNA interference) three-dimensional microscopy that localized at chromosome periphery during...
Cohesion is essential for the identification of sister chromatids and biorientation chromosomes until their segregation. Here, we have demonstrated that an RNA-binding motif protein encoded on X chromosome (RBMX) plays role in morphogenesis through its association with chromatin, but not RNA. Depletion RBMX by RNA interference (RNAi) causes loss cohesin from centromeric regions before anaphase, resulting premature chromatid separation accompanied delocalization shugoshin complex outer...
Growth and developmental processes are occasionally accompanied by multiple DNA replications, known as endoreduplication. Coordination between endoreduplication cell size regulation often plays a crucial role in proper organogenesis differentiation. Here, we report that the level of correlation ploidy volume is different outer inner layers leaves Arabidopsis thaliana using novel imaging technique. Although there well-known, strong pavement cells epidermis, this was extremely weak palisade...
Abstract Acquisition of pluripotency by somatic cells is a striking process that enables multicellular organisms to regenerate organs. This includes silencing genes erase original tissue memory and priming additional cell type specification genes, which are then poised for activation external signal inputs. Here, through analysis genome-wide histone modifications gene expression profiles, we show mechanism involving LYSINE-SPECIFIC DEMETHYLASE 1-LIKE 3 (LDL3) specifically eliminates H3K4me2...
Abstract Tissue clearing methods are increasingly essential for the microscopic observation of internal tissues thick biological organs. We previously developed TOMEI, a method plant tissues; however, it could not entirely remove chlorophylls nor reduce fluorescent signal proteins. Here, we an improved TOMEI (iTOMEI) to overcome these limitations. First, caprylyl sulfobetaine was determined efficiently from Arabidopsis thaliana seedlings without GFP quenching. Next, weak alkaline solution...
The subnuclear distribution of centromeres is cooperatively regulated by condensin II and the linker nucleoskeleton cytoskeleton (LINC) complex. However, other nuclear membrane structures proteins are probably involved in centromere dynamics distribution. Here, we focused on pore complex (NPC), which known to regulate gene expression, transcription memory, chromatin structure addition transport between cytoplasm nucleoplasm. We report here that some nucleoporins (Nups), including Nup85,...