A5000443198- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Light effects on plants
- Plant Parasitism and Resistance
- Plant biochemistry and biosynthesis
- Microtubule and mitosis dynamics
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Lipid metabolism and biosynthesis
- Protist diversity and phylogeny
- HIV Research and Treatment
- Transgenic Plants and Applications
- Cancer Diagnosis and Treatment
- Date Palm Research Studies
- Potato Plant Research
- Phytochemical Studies and Bioactivities
- Tree Root and Stability Studies
- Head and Neck Cancer Studies
Kumamoto University
2018-2023
Tokyo University of Science
2019
Nara Institute of Science and Technology
2008-2017
BioHybrid Solutions (United States)
2006
National Archives and Records Administration
2006
Utrecht University
2002-2005
Northwest African American Museum
2005
Joetsu University of Education
2000
Massachusetts General Hospital
2000
Harvard University
2000
Mutations in CUC1 and CUC2 (for CUP-SHAPED COTYLEDON), which are newly identified genes of Arabidopsis, caused defects the separation cotyledons (embryonic organs), sepals, stamens (floral organs) as well formation shoot apical meristems. These were most apparent double mutant. Phenotypes mutants suggest a common mechanism for separating adjacent organs within same whorl both embryos flowers. We cloned gene found that encoded protein was homologous to petunia NO APICAL MERISTEM (NAM)...
Abstract CUP-SHAPED COTYLEDON1 (CUC1), CUC2, and CUC3 define the boundary domain around organs in Arabidopsis thaliana meristem. CUC1 CUC2 transcripts are targeted by a microRNA (miRNA), miR164, encoded MIR164A, B, C. We show that each MIR164 is transcribed to generate large population of primary miRNAs variable size with locally conserved secondary structure pre-miRNA. identified mutations MIR164A gene deepen serration leaf margin. By contrast, leaves plants overexpressing miR164 have...
Abstract The shoot apical meristem and cotyledons of higher plants are established during embryogenesis in the apex. Redundant CUP-SHAPED COTYLEDON 1 (CUC1) CUC2 as well SHOOT MERISTEMLESS (STM) Arabidopsis required for formation cotyledon separation. To elucidate how region embryo is established, we investigated genetic interactions among CUC1, STM, expression patterns STM mRNA. Expression these genes marked incipient boundaries primordia, consistent with their roles Genetic analyses...
ABSTRACT Several lines of evidence indicate that the adaxial leaf domain possesses a unique competence to form shoot apical meristems. Factors required for this are expected cause defect in meristem formation when inactivated and be expressed or active preferentially domain. PINHEAD, member family proteins includes translation factor eIF2C, is reliable primary axillary In addition high-level expression vasculature, we find low-level PINHEAD defines novel positional identity plant. This...
Abstract Overall shoot architecture in higher plants is highly dependent on the activity of embryonic and axillary meristems, which are produced from basal adaxial boundaries cotyledons leaves, respectively. In Arabidopsis thaliana, redundant functions CUP-SHAPED COTYLEDON genes CUC1, CUC2, CUC3 regulate meristem formation cotyledon boundary specification. Their functional importance relationship postembryonic development, however, poorly understood. Here, we performed extensive analyses...
In dicotyledonous plants, two cotyledons are formed at bilaterally symmetric positions in the apical region of embryo. Single mutations PIN-FORMED1 (PIN1) and PINOID (PID) genes, which mediate auxin-dependent organ formation, moderately disrupt patterning cotyledons. We report that pin1 pid double mutant displays a striking phenotype completely lacks bilateral symmetry. embryo, expression domains CUP-SHAPED COTYLEDON1 (CUC1), CUC2 SHOOT MERISTEMLESS (STM), functions normally required to...
In dicotyledonous plants, the apical region of embryo shifts from radial to bilateral symmetry as two cotyledon primordia develop on opposite sides shoot meristem. To further elucidate mechanisms regulating this patterning process, we analyzed functions Arabidopsis genes, PIN-FORMED1 (PIN1) and MONOPTEROS (MP), encoding a putative auxin efflux carrier transcription factor thought mediate signaling, respectively. The corresponding mutants show similar defects in patterning, including fusion...
Here we report a novel Arabidopsis dwarf mutant, fackel-J79 , whose adult morphology resembles that of brassinosteroid-deficient mutants but also displays distorted embryos, supernumerary cotyledons, multiple shoot meristems, and stunted roots. We cloned the FACKEL gene found it encodes protein with sequence similarity to both human sterol reductase family yeast C-14 is preferentially expressed in actively growing cells. Biochemical analysis indicates fk-J79 mutation results deficient...
Two mutations in Arabidopsis thaliana, auxin response factor6 (arf6) and arf8, concomitantly delayed the elongation of floral organs subsequently opening flower buds. This phenotype is shared with jasmonic acid (JA)-deficient mutant dad1, and, indeed, JA level arf6 arf8 buds was decreased. Among biosynthetic genes, expression DAD1 (DEFECTIVE IN ANTHER DEHISCENCE1) markedly decreased double mutant, suggesting that ARF6 ARF8 are required for activation expression. The also showed other...
Abstract Organ primordia develop from founder cells into organs due to coordinated patterns of cell division. How patterned division is regulated during organ formation, however, not well understood. Here, we show that the PUCHI gene, which encodes a putative APETALA2/ethylene-responsive element binding protein transcription factor, required for pattern divisions lateral root formation in Arabidopsis thaliana. Recessive mutations disturbed primordium, resulting swelling proximal region...
Abstract Seed morphogenesis consists of embryogenesis and the development maternal tissues such as inner outer integuments, both which give rise to seed coats. We show that expression chimeric repressors derived from NAC-REGULATED SEED MORPHOLOGY1 -2 (NARS1 NARS2, also known NAC2 NAM, respectively) caused aberrant shapes in Arabidopsis thaliana. Double knockout mutant nars1 nars2 exhibited abnormally shaped seeds; moreover, neither nor produced abnormal seeds, indicating NARS1 NARS2...
The role of mechanical signals in cell identity determination remains poorly explored tissues. Furthermore, because stress is widespread, are difficult to uncouple from biochemical-based transduction pathways. Here we focus on the homeobox gene SHOOT MERISTEMLESS (STM), a master regulator and marker meristematic Arabidopsis. We found that STM expression quantitatively correlated curvature saddle-shaped boundary domain shoot apical meristem. As tissue folding reflects presence stress, test...
Summary The establishment of organ boundaries is a fundamental process for proper morphogenesis in multicellular organisms. In plants, the shoot meristem repetitively forms primordia from its periphery, and boundary cells are generated between them to separate their cellular fates. genes CUP‐SHAPED COTYLEDON1 ( CUC1 ) CUC2 , which encode plant‐specific NAC transcription factors, play central roles Arabidopsis thaliana . Here we show that protein activates expression LIGHT‐DEPENDENT SHORT...
Upon hormonal signaling, ovules develop as lateral organs from the placenta. Ovule numbers ultimately determine number of seeds that develop, and thereby contribute to final seed yield in crop plants. We demonstrate here CUP-SHAPED COTYLEDON 1 (CUC1), CUC2 AINTEGUMENTA (ANT) have additive effects on ovule primordia formation. show expression CUC1 genes is required redundantly regulate PINFORMED1 (PIN1), which turn for Furthermore, our results suggest auxin response factor MONOPTEROS...
Carpel margin meristems (CMMs), a pair of meristematic tissues present along the margins two fused carpel primordia Arabidopsis thaliana, are essential for formation ovules and septum, major internal structures gynoecium. Although number regulatory factors involved in shoot meristem activity known to be required these gynoecial structures, their direct roles CMM development have yet addressed. Here we show that CUP-SHAPED COTYLEDON genes CUC1 CUC2, which initiation, also stable positioning...
Clearing techniques eliminate factors that interfere with microscopic observation, including light scattering and absorption by pigments cytoplasmic components. The allow fluorescence-based detailed analyses of materials characterization the three-dimensional structure organs. We describe a simple rapid clearing imaging method, termed 'TOMEI' (Transparent plant Organ MEthod for Imaging), which enables observation intact This method involves reagent containing 2,2′-thiodiethanol....
When mutations in CUP-SHAPED COTYLEDON1 (CUC1) and CUC2 are combined, severe defects involving fusion of sepals stamens occur Arabidopsis flowers. In addition, septa gynoecia do not fuse along the length ovaries many ovules have their growth arrested. is expressed at tips septal primordia during gynoecium development boundary between nucellus chalaza ovule development. These expression patterns partially consistent with phenotype mutant gynoecium. mRNA also shown to be boundaries meristems...
Possible links between plant defense responses and morphogenesis have been postulated, but their molecular nature remains unknown. Here, we introduce the Arabidopsis semi-dominant mutant uni-1D with morphological defects. UNI encodes a coiled-coil nucleotide-binding leucine-rich-repeat protein that belongs to disease resistance (R) family involved in pathogen recognition. The mutation causes constitutive activation of protein, which is stabilized by RAR1 function similar way as other R...
The evolution of plant reproductive strategies has led to a remarkable diversity structures, especially within the flower, structure characteristic angiosperms. In flowering plants, sexual reproduction depends notably on development gynoecium that produces and protects ovules. Arabidopsis thaliana, ovule initiation is promoted by concerted action auxin with CUC1 (CUP-SHAPED COTYLEDON1) CUC2, two genes encode transcription factors NAC family (NAM/ATAF1,2/CUC). Here we highlight an additional...
Abstract Acquisition of pluripotency by somatic cells is a striking process that enables multicellular organisms to regenerate organs. This includes silencing genes erase original tissue memory and priming additional cell type specification genes, which are then poised for activation external signal inputs. Here, through analysis genome-wide histone modifications gene expression profiles, we show mechanism involving LYSINE-SPECIFIC DEMETHYLASE 1-LIKE 3 (LDL3) specifically eliminates H3K4me2...