A5040590746- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant Pathogenic Bacteria Studies
- Plant Gene Expression Analysis
- Light effects on plants
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Transgenic Plants and Applications
- RNA and protein synthesis mechanisms
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Metabolism, Diabetes, and Cancer
- Plant Parasitism and Resistance
- Plant responses to water stress
- Fungal and yeast genetics research
- Pancreatic function and diabetes
- Lipid metabolism and biosynthesis
- Plant Physiology and Cultivation Studies
- Postharvest Quality and Shelf Life Management
Harvard University
2016-2025
Massachusetts General Hospital
2016-2025
Baylor Genetics
2017
Boston University
1995-2014
Advisory Board Company (United States)
2011
New York Proton Center
2011
Parker Hannifin (Germany)
2011
RELX Group (United Kingdom)
2011
Wageningen University & Research
2011
Communities In Schools of Orange County
2011
Despite the recognition of H 2 O as a central signaling molecule in stress and wounding responses, pathogen defense, regulation cell cycle death, little is known about how signal perceived transduced plant cells. We report here that potent activator mitogen-activated protein kinases (MAPKs) Arabidopsis leaf Using epitope tagging protoplast transient expression assay, we show can activate specific kinase kinase, ANP1, which initiates phosphorylation cascade involving two MAPKs, AtMPK3 AtMPK6....
Glucose modulates many vital processes in photosynthetic plants. Analyses of Arabidopsis glucose insensitive2 (gin2) mutants define the physiological functions a specific hexokinase (HXK1) plant glucose-signaling network. HXK1 coordinates intrinsic signals with extrinsic light intensity. lacking catalytic activity still support various signaling gene expression, cell proliferation, root and inflorescence growth, leaf expansion senescence, thus demonstrating uncoupling from metabolism. The...
The mechanisms by which higher plants recognize and respond to sugars are largely unknown. Here, we present evidence that the first enzyme in hexose assimilation pathway, hexokinase (HXK), acts as a sensor for plant sugar responses. Transgenic Arabidopsis expressing antisense (AtHXK) genes hyposensitive, whereas overexpressing AtHXK hypersensitive. transgenic exhibited wide spectrum of altered responses seedling development gene activation repression. Furthermore, yeast YHXK2 caused dominant...
Glc has hormone-like functions and controls many vital processes through mostly unknown mechanisms in plants. We report here on the molecular cloning of GLUCOSE INSENSITIVE1 (GIN1) ABSCISIC ACID DEFICIENT2 (ABA2) which encodes a unique Arabidopsis short-chain dehydrogenase/reductase (SDR1) that as link between nutrient signaling plant hormone biosynthesis. SDR1 is related to SDR superfamily members involved retinoid steroid biosynthesis mammals sex determination maize. antagonizes ethylene...
Sugar repression of photosynthetic genes is likely a central control mechanism mediating energy homeostasis in wide range algae and higher plants. It overrides light activation coupled to developmental environmental regulations. How sugar signals are sensed transduced the nucleus remains unclear. To elucidate sugar-sensing mechanisms, we monitored effects variety sugars, glucose analogs, metabolic intermediates on fusion sensitive versatile maize protoplast transient expression system. The...
Using freshly isolated maize mesophyll protoplasts and a transient expression method, I showed that the transcriptional activity of seven photosynthetic gene promoters is specifically coordinately repressed by end products sucrose glucose exogenous carbon source acetate. Analysis deleted, mutated, hybrid sugars acetate inhibit distinct positive upstream regulatory elements without common consensus. The metabolic repression genes overrides other forms regulation, e.g., light, tissue type,...
Abstract Plant protoplasts show physiological perceptions and responses to hormones, metabolites, environmental cues, pathogen-derived elicitors, similar cell-autonomous in intact tissues plants. The development of defined protoplast transient expression systems for high-throughput screening systematic characterization gene functions has greatly contributed elucidating plant signal transduction pathways, combination with genetic, genomic, transgenic approaches.
Stress responses in plants involve changes the transcription of specific genes. The constitutively active mutants two related Ca 2+ -dependent protein kinases (CDPK1 and CDPK1a) activate a stress-inducible promoter, bypassing stress signals. Six other plant kinases, including distinct CDPKs, fail to mimic this signaling. activation is abolished by CDPK1 mutation kinase domain diminished phosphatase 2C that capable blocking hormone abscisic acid. A variety functions are mediated different...