A5075116238- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Cancer-related molecular mechanisms research
- Advanced Vision and Imaging
- Plant Pathogenic Bacteria Studies
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Digital Holography and Microscopy
- HER2/EGFR in Cancer Research
- Advanced Optical Imaging Technologies
- Circular RNAs in diseases
- Postharvest Quality and Shelf Life Management
- Plant Reproductive Biology
- Fungal and yeast genetics research
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Plant tissue culture and regeneration
- Advanced Image Processing Techniques
- Plant Virus Research Studies
- Immune Cell Function and Interaction
- Cancer Treatment and Pharmacology
Second Affiliated Hospital of Xi'an Jiaotong University
2016-2025
Electric Power University
2025
Northeast Electric Power University
2025
Wuyi University
2024-2025
Fujian Agriculture and Forestry University
2024-2025
Zhongshan People's Hospital
2025
University of Missouri
2015-2024
Third Affiliated Hospital of Southern Medical University
2024
Xi'an Jiaotong University
2014-2023
Second Hospital of Shandong University
2023
Abstract Mitogen-activated protein kinases (MAPKs) are implicated in regulating plant growth, development, and response to the environment. However, underlying mechanisms unknown because of lack information about their substrates. Using a conditional gain-of-function transgenic system, we demonstrated that activation SIPK, tobacco (Nicotiana tabacum) stress-responsive MAPK, induces biosynthesis ethylene. Here, report MPK6, Arabidopsis thaliana ortholog is required for ethylene induction this...
Abstract Stomata are specialized epidermal structures that regulate gas (CO2 and O2) water vapor exchange between plants their environment. In Arabidopsis thaliana, stomatal development is preceded by asymmetric cell divisions, distribution follows the one-cell spacing rule, reflecting coordination of fate specification. Stomatal patterning regulated both genetic environmental signals. Here, we report MITOGEN-ACTIVATED PROTEIN KINASE3 (MPK3) MPK6, two environmentally responsive...
Abstract Plant sensing of invading pathogens triggers massive metabolic reprogramming, including the induction secondary antimicrobial compounds known as phytoalexins. We recently reported that MPK3 and MPK6, two pathogen-responsive mitogen-activated protein kinases, play essential roles in camalexin, major phytoalexin Arabidopsis thaliana. In search transcription factors downstream MPK3/MPK6, we found WRKY33 is required for MPK3/MPK6-induced camalexin biosynthesis. wrky33 mutants, both...
A new impulse noise detection technique for switching median filters is presented, which based on the minimum absolute value of four convolutions obtained using one-dimensional Laplacian operators. Extensive simulations show that proposed filter provides better performance than many existing with comparable computational complexity. In particular, directed toward improved line preservation.
The involvement of phosphorylation/dephosphorylation in the salicylic acid (SA) signal transduction pathway leading to pathogenesis-related gene induction has previously been demonstrated using kinase and phosphatase inhibitors. Here, we show that tobacco suspension cells, SA induced a rapid transient activation 48-kD uses myelin basic protein as substrate. This is called p48 SIP (for SA-Induced Protein kinase). Biologically active analogs SA, which induce genes enhanced resistance, also...
Abstract In plants and animals, induced resistance (IR) to biotic abiotic stress is associated with priming of cells for faster stronger activation defense responses. It has been hypothesized that cell involves accumulation latent signaling components are not used until challenge exposure stress. However, the identity such remained elusive. Here, we show during development chemically in Arabidopsis thaliana, mRNA inactive proteins mitogen-activated protein kinases (MPKs), MPK3 MPK6. Upon or...
Hypersensitive response (HR), a form of programmed cell death, is frequently associated with plant disease resistance. It has been proposed that mitogen-activated protein kinase (MAPK) cascades regulate HR death based on pharmacological studies by using inhibitors. However, direct evidence lacking. Here, we demonstrate NtMEK2, MAPK kinase, upstream salicylic acid-induced (SIPK) and wounding-induced (WIPK), two tobacco MAPKs are activated various pathogens or pathogen-derived elicitors....
The Cf-9 resistance (R) gene from tomato confers to the fungal pathogen Cladosporium fulvum expressing corresponding, pathogen-derived avirulence product Avr9. To understand how an initial R/Avr recognition event is transmitted and triggers induction of plant defenses, we investigated early Avr9/Cf-9-dependent activation protein kinases in transgenic tobacco gene. We identified two 46 48 kD, using myelin basic as substrate, that became rapidly activated a strictly gene-for-gene manner within...
Rapid and localized programmed cell death, known as the hypersensitive response (HR) is frequently associated with plant disease resistance. In contrast to our knowledge about regulation execution of apoptosis in animal system, information HR limited. Recent studies implicated mitogen-activated protein kinase (MAPK) cascade regulating death well several other defense responses during incompatible interactions between plants pathogens. Here, we report generation transgenic <i>Arabidopsis</i>...
Abstract Plant responses to ethylene are mediated by regulation of EBF1/2-dependent degradation the ETHYLENE INSENSITIVE3 (EIN3) transcription factor. Here, we report that level EIL1 protein is upregulated through an pathway. Genetic analysis revealed and EIN3 cooperatively but differentially regulate a wide array responses, with mainly inhibiting leaf expansion stem elongation in adult plants largely regulating multitude seedlings. When EBF1 EBF2 disrupted, constitutively accumulate nucleus...
Plants under pathogen attack produce high levels of ethylene, which plays important roles in plant immunity. Previously, we reported the involvement ACS2 and ACS6, two Type I ACS isoforms, Botrytis cinerea–induced ethylene biosynthesis their regulation at protein stability level by MPK3 MPK6, Arabidopsis pathogen-responsive mitogen-activated kinases (MAPKs). The residual induction acs2/acs6 double mutant suggests additional isoforms. It is also known that a subset genes, including...
Abstract Arabidopsis thaliana MPK3 and MPK6, two mitogen-activated protein kinases (MAPKs or MPKs), play critical roles in plant disease resistance by regulating multiple defense responses. Previously, we characterized the regulation of phytoalexin biosynthesis MPK3/MPK6 cascade its downstream WRKY33 transcription factor. Here, report another substrate MPK3/MPK6, ETHYLENE RESPONSE FACTOR6 (ERF6), gene expression to necrotrophic fungal pathogen Botrytis cinerea. Phosphorylation ERF6 either...
Plant recognition of pathogens leads to rapid activation MPK3 and MPK6, two Arabidopsis mitogen-activated protein kinases (MAPKs), their orthologs in other species. Here, we report that synthesis camalexin, the major phytoalexin Arabidopsis, is regulated by MPK3/MPK6 cascade. Activation expression active upstream MAPK kinase (MAPKK) or MAPKK (MAPKKK) was sufficient induce camalexin absence pathogen attack. Induction Botrytis cinerea preceded activation, compromised mpk3 mpk6 mutants. Genetic...
The Arabidopsis (Arabidopsis thaliana) gene MEKK1 encodes a mitogen-activated protein kinase that has been implicated in the activation of map kinases MPK3 and MPK6 response to flagellin elicitor peptide flg22. In this study, analysis plants carrying T-DNA knockout alleles indicated is required for flg22-induced MPK4 but not or MPK6. Experiments performed using kinase-impaired version (K361M) showed activity may be other macroscopic FLS2-mediated responses. play structural role signaling,...
Abscission is a developmental program that results in the active shedding of infected or nonfunctional organs from plant body. Here, we establish signaling pathway controls abscission Arabidopsis thaliana ligand, to receptors, downstream effectors. Loss function mutations Inflorescence Deficient (IDA), which encodes predicted secreted small protein, receptor-like protein kinases HAESA (HAE) and HAESA-like 2 (HSL2), Mitogen-Activated Protein Kinase 4 (MKK4) MKK5, dominant-negative form 6...
Plant defense against pathogens often includes rapid programmed cell death known as the hypersensitive response (HR). Recent genetic studies have demonstrated involvement of a specific mitogen-activated protein kinase (MAPK) cascade consisting three tobacco MAPKs, SIPK, Ntf4 and WIPK, their common upstream MAPK (MAPKK or MEK), NtMEK2. Potential MAPKK kinases (MAPKKKs MEKKs) in this include orthologs Arabidopsis MEKK1 tomato MAPKKKalpha. Activation SIPK/Ntf4/WIPK pathway induces with...