A5039220474- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Microtubule and mitosis dynamics
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Plant Parasitism and Resistance
- Cellular transport and secretion
- Advanced Proteomics Techniques and Applications
- Food composition and properties
- Microbial Metabolites in Food Biotechnology
- Fungal and yeast genetics research
- Autophagy in Disease and Therapy
- Fungal Biology and Applications
- Genomics, phytochemicals, and oxidative stress
- Postharvest Quality and Shelf Life Management
- Endoplasmic Reticulum Stress and Disease
- Silk-based biomaterials and applications
- Sphingolipid Metabolism and Signaling
- Biofuel production and bioconversion
China Agricultural University
2015-2024
State Key Laboratory of Plant Physiology and Biochemistry
2016-2017
South China Institute of Collaborative Innovation
2017
University of Minnesota
2012
University of Missouri
2002-2008
Rapid and localized programmed cell death, known as the hypersensitive response (HR) is frequently associated with plant disease resistance. In contrast to our knowledge about regulation execution of apoptosis in animal system, information HR limited. Recent studies implicated mitogen-activated protein kinase (MAPK) cascade regulating death well several other defense responses during incompatible interactions between plants pathogens. Here, we report generation transgenic <i>Arabidopsis</i>...
Mitogen-activated protein kinase (MAPK) cascades play important roles in regulating plant growth, development, and responses to various environmental stimuli. We demonstrate that MKK9, an MKK, is upstream activator of the MPKs MPK3 MPK6 both vitro planta. Expression active MKK9 transgenic plants induces synthesis ethylene camalexin through activation endogenous kinases. As a consequence, transcription multiple genes responsible for biosynthesis, responses, biosynthesis coordinately...
Plant recognition of pathogens leads to rapid activation MPK3 and MPK6, two Arabidopsis mitogen-activated protein kinases (MAPKs), their orthologs in other species. Here, we report that synthesis camalexin, the major phytoalexin Arabidopsis, is regulated by MPK3/MPK6 cascade. Activation expression active upstream MAPK kinase (MAPKK) or MAPKK (MAPKKK) was sufficient induce camalexin absence pathogen attack. Induction Botrytis cinerea preceded activation, compromised mpk3 mpk6 mutants. Genetic...
Plant defense against pathogens often includes rapid programmed cell death known as the hypersensitive response (HR). Recent genetic studies have demonstrated involvement of a specific mitogen-activated protein kinase (MAPK) cascade consisting three tobacco MAPKs, SIPK, Ntf4 and WIPK, their common upstream MAPK (MAPKK or MEK), NtMEK2. Potential MAPKK kinases (MAPKKKs MEKKs) in this include orthologs Arabidopsis MEKK1 tomato MAPKKKalpha. Activation SIPK/Ntf4/WIPK pathway induces with...
Abstract Nitric oxide (NO) is a bioactive molecule that functions in numerous physiological and developmental processes plants, including lateral root development. In this study, we used biochemical genetic approaches to analyze the function of Arabidopsis thaliana mitogen-activated protein kinase 6 (MPK6) regulation NO synthesis response hydrogen peroxide (H2O2) during both mpk6 mutants studied, H2O2-induced nitrate reductase (NR) activity were decreased dramatically. Furthermore, one NR...
Abstract The successful recognition of pathogen-associated molecular patterns (PAMPs) as a danger signal is crucial for plants to fend off numerous potential pathogenic microbes. relayed through mitogen-activated protein kinase (MPK) cascades activate defenses. Here, we show that the Pseudomonas syringae type III effector HopF2 can interact with Arabidopsis thaliana MAP KINASE KINASE5 (MKK5) and likely other MKKs inhibit MPKs PAMP-triggered immunity. Inhibition PAMP-induced MPK...
Summary Plant recognition of pathogen‐associated molecular patterns ( PAMP s) such as bacterial flagellin‐derived flg22 triggers rapid activation mitogen‐activated protein kinases MAPK and generation reactive oxygen species ROS ). Arabidopsis has at least four /pathogen‐responsive s: MPK 3, 6, 4 11. It was speculated that these s may function downstream in plant immunity because their by exogenously added H 2 O . 3/ 6 or orthologs other have also been reported to be involved the burst from...
The mitogen-activated protein kinase (MAPK) cascade is an evolutionarily conserved signal transduction module involved in transducing extracellular signals to the nucleus for appropriate cellular adjustment. This essentially consists of three components: a MAPK (MAPKKK), kinase, and MAPK, connected each other by event phosphorylation. Here, we report characterization MAPKKK, ABA-INSENSITIVE PROTEIN KINASE1 (AIK1), which regulates abscisic acid (ABA) responses Arabidopsis (Arabidopsis...
Abstract Camalexin, a major phytoalexin in Arabidopsis thaliana, consists of an indole ring and thiazole ring. The is produced from Trp, which converted to indole-3-acetonitrile (IAN) by CYP79B2/CYP79B3 CYP71A13. Conversion Cys(IAN) dihydrocamalexic acid subsequently camalexin catalyzed CYP71B15. Recent studies proposed that Cys derivative, not itself, the precursor conjugates with IAN. nature derivative how it IAN forms remain obscure. We found protein accumulation multiple glutathione...
Arabidopsis calcium-dependent protein kinase CPK6 positively regulates seed germination, seedling growth, and drought tolerance via phosphorylating ABF ABI5 transcription factors.
The chloroplast-localized SIB1 protein was previously identified by its interaction with SIGMA FACTOR 1 (SIG1), a component of the RNA polymerase machinery responsible for transcription plastid genes. physiological function is little known. We found that expression induced infection Pseudomonas syringae, suggesting possible involvement in defence response. sib1 loss-of-function mutation compromises induction some defence-related genes triggered pathogen and treatments salicylic acid (SA)...
The Arabidopsis copine gene BON1 encodes a calcium-dependent phospholipid-binding protein involved in plant growth homeostasis and disease resistance. However, the biochemical molecular mechanisms by which modulates defense responses are not well understood. Here, we show that interacts physically with leucine-rich-repeat receptor-like kinases BIR1 (BAK1-interacting kinase 1) pathogen-associated pattern (PAMP) receptor regulator BAK1 vitro vivo. Additionally, bon1 bir1 mutants exhibit...
Abstract Chloroplast retrograde signals play important roles in coordinating the plastid and nuclear gene expression are critical for proper chloroplast biogenesis maintaining optimal functions response to environmental changes plants. Until now, mechanisms signalling remain poorly understood. Here we identify factors that allow nucleus perceive stress conditions respond accordingly by inducing or repressing specific genes encoding proteins. We show ABI4, which is known repress LHCB during...
Male sterility (MS) provides a useful breeding tool to harness hybrid vigor for seed production. It is necessary generate new male sterile mutant lines the development of production technology. The CRISPR/Cas9 technology well suited targeting genomes mutants. In this study, we artificially synthesized Streptococcus pyogenes Cas9 gene with biased codons maize. A vector MS8 maize was constructed and transformed into using an Agrobacterium-mediated method, eight T0 independent transgenic were...
Germ cells are indispensable carriers of genetic information from one generation to the next. In contrast well-understood process in animals, on mechanism germ cell initiation plants is very limited. SPOROCYTELESS/NOZZLE was previously identified as an essential regulator diploid (archesporial cell) differentiation stamens and ovules Arabidopsis (Arabidopsis thaliana). Although SPOROCYTELESS (SPL) transcription activated by floral organ identity AGAMOUS epigenetically regulated SET DOMAIN...
Abstract Ethylene response factor (ERF) Group VII members generally function in regulating plant growth and development, abiotic stress responses, immunity Arabidopsis; however, the details of regulatory mechanism by which ERFs mediate immune responses remain elusive. Here, we characterized one such member, ERF72, as a positive regulator that mediates resistance to necrotrophic pathogen Botrytis cinerea. Compared with wild-type (WT), erf72 mutant showed lower camalexin concentration was more...
Mitogen-activated protein kinase (MAPK) cascades are important signaling modules in eukaryotic cells. They function downstream of sensors/receptors and regulate cellular responses to external endogenous stimuli. Recent studies demonstrated that SIPK WIPK, two tobacco (Nicotiana spp.) MAPKs, involved plant defense various pathogens. Ntf4, another MAPK shares 93.6% 72.3% identity with respectively, was reported be developmentally regulated pollen germination. We found Ntf4 is also expressed...
Many biological processes require the co-operative involvement of both microtubules and microfilaments; however, only a few proteins mediating interaction between microfilaments have been identified from plants. In present study, cotton kinesin GhKCH2, which contains CH (calponin homology) domain at N-terminus, was analysed in vitro vivo order to understand its with two cytoskeletal elements. A specific antibody against GhKCH2 prepared used for immunolabelling experiments. Some spots...
Abstract The involvement of cytoskeleton-related proteins in regulating mitochondrial respiration has been revealed mammalian cells. However, it is unclear if there a relationship between the microtubule-based motor protein kinesin and respiration. In this research, we demonstrate that plant-specific kinesin, Kinesin-like 1 (KP1; At KIN14 h), involved respiratory regulation during seed germination at low temperature. Using vitro biochemical methods vivo transgenic cell observations, KP1 able...
Despite the abundance of phosphorus in soil, very little is available as phosphate (Pi) for plants. Plants often experience low Pi (LP) stress. Intensive studies have been conducted to reveal mechanism used by plants deal with LP; however, sensing and signal transduction pathways are not fully understood. Using in-gel kinase assays, we determined activities MPK3 MPK6 Arabidopsis thaliana seedlings under both LP Pi-sufficient (Murashige Skoog, MS) conditions. MKK9 mutant transgenic crossed...
Abstract In plants, cell morphogenesis is dependent on intercellular auxin accumulation. The polar subcellular localization of the PIN-FORMED (PIN) protein crucial for this process. Previous studies have shown that kinase PINOID (PID) and phosphatase6-type phosphatase holoenzyme regulate phosphorylation status PIN1 in root tips shoot apices. Here, we show a type-one phosphatase, TOPP4, essential formation interdigitated pavement (PC) pattern Arabidopsis (Arabidopsis thaliana) leaf....
MAPK signaling pathways play critical roles in plant immunity. Here, we silenced multiple genes encoding MAPKs using virus-induced gene silencing mediated by Bean pod mottle virus to identify involved soybean (Glycine max) Surprisingly, a strong hypersensitive response (HR) cell death was observed when KINASE KINASE1 (GmMEKK1), homolog of Arabidopsis (Arabidopsis thaliana) MEKK1, silenced. The HR accompanied the overaccumulation defense molecules, salicylic acid (SA) and hydrogen peroxide....
Summary Mitogen‐activated protein kinase (MAPK) cascades play vital roles in regulating plant growth, development, and stress responses. MAPK‐like (MPKL) proteins are a group of kinases containing the MAPK signature TxY motif showing sequence similarity to MAPKs. However, functions MPKL currently unknown. The maize ( Zea mays ) genome contains four genes encoding proteins, here named ZmMPKL1 ZmMPKL4 . In this study, we show that possesses activity drought‐induced expression, overexpression...