A5100768487- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Biosensors and Analytical Detection
- Electrocatalysts for Energy Conversion
- Advanced Fiber Optic Sensors
- Advanced battery technologies research
- Gold and Silver Nanoparticles Synthesis and Applications
- DNA Repair Mechanisms
- Conducting polymers and applications
- RNA modifications and cancer
- Electrochemical Analysis and Applications
- Electrochemical sensors and biosensors
- Quantum Chromodynamics and Particle Interactions
- Machine Learning in Bioinformatics
- High-Energy Particle Collisions Research
- Semiconductor Lasers and Optical Devices
- Molecular Biology Techniques and Applications
- Animal Genetics and Reproduction
- Advanced Biosensing Techniques and Applications
- Particle physics theoretical and experimental studies
Nanjing Tech University
2024-2025
Materials Science & Engineering
2025
Sun Yat-sen University
2007-2025
Nantong University
2023-2025
Shanxi Academy of Medical Sciences
2024-2025
Shanxi Medical University
2024-2025
Agency for Science, Technology and Research
2023-2025
Institute of Materials Research and Engineering
2023-2025
Guangdong Medical College
2025
State Key Laboratory of Synthetic Chemistry
2025
Herein, a visual electrochemiluminescence (ECL) luminophore, 6-azido-2-thioxanthine-coated gold nanoclusters (ATT-Au NCs), was prepared efficiently in single step, followed by comprehensive characterization of their structural, optical, and ECL properties using diverse analytical methodologies. Concurrently, nanoparticles, dimers, nanorod (Au NR) dispersions, dimers (parallel perpendicular conformations) were synthesized via chemical reduction, DNA ligation, seed growth, electrostatic...
One of the “simple” curiosities life, at least for a biological scientist, is how growth and development complete organism from single cell controlled. Part answer includes factor receptors their respective ligands, which transduce signals across membranes into nucleus transcriptional readout. The molecular nature these intracellular determines type signals, pathways involved, subsequent regulatory interactions, eventual transcription activation/repression. Hematopoietic has been fruitful system
Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model development livestock breeding. Despite ongoing attempts since the 1990s, no stably defined pig cell line has been established. Here, guided by insights from a large-scale single-cell transcriptome analysis of embryos embryonic day (E) 0 E14, specifically, tracing pluripotency changes during epiblast development, we developed an in vitro culture medium...
An all-electrochem-active Si/Li 21 Si 5 anode was designed, in which the Li served as efficient ionic/electronic channel and expansion buffer. It achieved an ultra-high ICE of 97.8% a low rate 18.8% at capacity 17.9 mA h cm −2 .
A multicolor electrochemiluminescence (ECL) biosensor based on a closed bipolar electrode (BPE) array was proposed for the rapid and intuitive analysis of three prostate cancer staging indicators. First, [Irpic-OMe], [Ir(ppy)2(acac)], [Ru(bpy)3]2+ were applied as blue, green, red ECL emitters, respectively, whose mixed emission colors covered whole visible region by varying voltages. Afterward, we designed simple Mg2+-dependent DNAzyme (MNAzyme)-driven tripedal DNA walker (TD walker) to...
Abstract Solid‐state quantum emitters are essential sources of single photons, and enhancing their emission rates is paramount importance for applications in communications, computing, metrology. One approach to couple with resonant photonic nanostructures, where the rate enhanced due Purcell effect. Dielectric nanoantennas promising as they provide strong enhancement compared plasmonic ones, which suffer from high Ohmic loss. Here, a dielectric Fano resonator designed fabricated based on...
Male sterility (MS) provides a useful breeding tool to harness hybrid vigor for seed production. It is necessary generate new male sterile mutant lines the development of production technology. The CRISPR/Cas9 technology well suited targeting genomes mutants. In this study, we artificially synthesized Streptococcus pyogenes Cas9 gene with biased codons maize. A vector MS8 maize was constructed and transformed into using an Agrobacterium-mediated method, eight T0 independent transgenic were...
Constructing active heterointerfaces is powerful to enhance the electrochemical performances of transition metal dichalcogenides, but interface density regulation remains a huge challenge. Herein, MoO2 /MoS2 heterogeneous nanorods are encapsulated in nitrogen and sulfur co-doped carbon matrix (MoO2 @NSC) by controllable sulfidation. MoS2 coupled intimately at atomic level, forming with different distribution density. Strong electronic interactions triggered these for enhancing electron...
Abstract A 3D ordered macroporous (3DOM) ionic‐liquid‐doped polyaniline (IL‐PANI) inverse opaline film is fabricated with an electropolymerization method and gold nanoparticles (AuNPs) are assembled on the by electrostatic adsorption, which offers a promising basis for biomolecular immobilization due to its satisfactory chemical stability, good electronic conductivity, excellent biocompatibility. The AuNP/IL‐PANI could be used fabricate electrochemical impedance spectroscopy (EIS)...
An ultrasensitive SPME probe based on phenylboronic acid functionalized CNTs is applied for direct<italic>in vitro</italic>or<italic>in vivo</italic>recognition of carbohydrates in biofluids as well semi-solid biotissues.
Abstract A variety of nanodevices developed for nucleic acid computation provide great opportunities to construct versatile synthetic circuits manipulation gene expressions. In our study, by employing a two-hairpin mediated strand displacement as processing joint conditional guide RNA, we aim build artificial connections between naturally occurring RNA expressions through programmable CRISPR/Cas9 function. This possesses sequence-switching machinery, in which random trigger can be processed...
Abstract Smart window is promising to save building energy and reduce carbon emissions. The fast development leads a high demand for multifunctionality not limited saving, while the material design fabrication are challenging. Herein, scalable method developed tri‐mode light regulations: thermo‐, mechano‐, hydro‐/solvato‐chromisms. film constructed of bio‐inspired hierarchical‐structured surface functional elastomer base. Through combined experiments simulations, triple‐stimuli‐chromic...
Cervical cancer is the third most common threatening women’s health globally, and high-risk human papillomavirus (HR-HPV) infection main cause of cervical worldwide. Given recurrent nature HR-HPV infection, accurate screening essential for its control. Since commonly used polymerase chain reaction (PCR) technique limited by professional equipment personnel, convenient ultrasensitive detection methods are still highly needed. As new molecular methods, nucleic acid amplification-based...
BACKGROUND Perineural invasion (PNI) has been used as an important pathological indicator and independent prognostic factor for patients with rectal cancer (RC). Preoperative prediction of PNI status is helpful individualized treatment RC. Recently, several radiomics studies have to predict the in RC, demonstrating a good predictive effect, but results lacked generalizability. The preoperative still challenging needs further study. AIM To establish validate optimal model predicting...
Abstract Phase engineering is promising to increase the intrinsic activity of catalyst toward hydrogen evolution reaction (HER). However, polymorphism interface unstable due presence metastable phases. Herein, phase and dispersion stabilization are applied simultaneously boost HER cobalt without sacrificing stability. A fast facile approach (plasma cathodic electro deposition) developed prepare film with a hetero‐phase structure. The polymorphs realized through reduced stacking fault energy...
This paper demonstrates a novel strategy for site-selective cell adhesion and in situ cultivation of living cells, integrated with real-time monitoring cellular small biomolecules based on dual functional protein microarrays. The microarrays have been produced the superhydrophobic|philic Au−TiO2 micropatterns, through further modification l-cysteine (Cys) followed by successive immobilization model protein, cytochrome c (cyt c). Experimental results revealed that created cyt play functions:...
Here we demonstrate a facile strategy of preparing gold nanoparticle (AuNP) and DNA conjugates by in situ generation strong metal affinity capping ligands, dithiocarbamates (DTC) modified oligonucleotides; the produced are stable at elevated temperature, resistant to ligand displacement preserve functionality conjugated oligonucleotides.
Abstract Shank proteins, one of the principal scaffolds in postsynaptic density (PSD) glutamatergic synapses, have been associated with autism spectrum disorders and neuropsychiatric diseases. However, it is not known whether different family proteins distinct functions regulating synaptic transmission, how they differ from other scaffold this aspect. Here, we investigate role Shanks transmission at rat hippocampal SC-CA1 using lentivirus-mediated knockdown molecular replacement combined...
Precise genome editing in livestock is of great value for the fundamental investigation disease modeling. However, genetically modified pigs carrying subtle point mutations were still seldom reported despite rapid development programmable endonucleases. Here, we attempt to investigate single-stranded oligonucleotides (ssODN) mediated knockin by introducing two orthologous pathogenic mutations, p.E693G Alzheimer's and p.G2019S Parkinson's disease, into porcine APP LRRK2 loci, respectively....