A5100745302- Genetic Mapping and Diversity in Plants and Animals
- Plant Molecular Biology Research
- Genetics and Plant Breeding
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Genetic and phenotypic traits in livestock
- RNA modifications and cancer
- Biofuel production and bioconversion
- Immune Cell Function and Interaction
- Insect Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Mitochondrial Function and Pathology
- Seed Germination and Physiology
- Plant Genetic and Mutation Studies
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Genetically Modified Organisms Research
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
Institute of Crop Sciences
2016-2025
Anhui University of Science and Technology
2024-2025
Chinese Academy of Agricultural Sciences
2016-2025
The Fourth People's Hospital
2024
Xian Yang Central Hospital
2024
Zhejiang A & F University
2024
Sanya University
2023
China Agricultural University
2000-2020
RELX Group (United States)
2019
University of Utah
1997-2016
Disease resistance (R) proteins, as central regulators of plant immunity, are tightly regulated for effective defense responses and to prevent constitutive activation under non-pathogenic conditions. Here we report the identification an F-box protein CPR1/CPR30 a negative regulator R SNC1 likely through SCF (Skp1-cullin-F-box) mediated degradation. The cpr1-2 (cpr30-1) loss-of-function mutant has responses, it interacts synergistically with gain-of function snc1-1 bon1-1 where is...
Raffinose and its precursor galactinol accumulate in plant leaves during abiotic stress. RAFFINOSE SYNTHASE (RAFS) catalyzes raffinose formation by transferring a galactosyl group of to sucrose. However, whether RAFS contributes drought tolerance and, if so, what mechanism remains unclear. In this study, we report that expression from maize (or corn, Zea mays) (ZmRAFS) is induced drought, heat, cold, salinity stresses. We found zmrafs mutant plants completely lack hyper-accumulate are more...
With the decrease of cost in genotyping, single nucleotide polymorphisms (SNPs) have gained wide acceptance because their abundance, even distribution throughout maize (Zea mays L.) genome, and suitability for high-throughput analysis. In this study, a 55 K SNP array with improved genome coverage molecular breeding was developed on an Affymetrix® Axiom® platform 55,229 SNPs evenly distributed across including 22,278 exonic 19,425 intronic SNPs. This contains 451 markers that are associated...
Abstract Background The maize inbred line A188 is an attractive model for elucidation of gene function and improvement due to its high embryogenic capacity many contrasting traits the first reference genome, B73, other elite lines. lack a genome assembly limits use as functional studies. Results Here, we present chromosome-level using long reads optical maps. Comparison with B73 both whole-genome alignments read depths from sequencing identify approximately 1.1 Gb syntenic sequences well...
Systemic Lupus Erythematosus (SLE) is a typical autoimmune disease characterized by complex pathogenesis and strong genetic predisposition. The study of inflammatory response in SLE monocytes not very clear, exploring the factors beneficial to discover new diagnostic targets. Using scRNA-seq technology, we obtained quantitative changes circulating immune cells various cellular metabolic profiles between patients healthy volunteers. A significant increase was observed peripheral blood...
A central question in neural development is how the broad diversity of neurons generated vertebrate CNS. We have investigated function Hoxc10 and Hoxd10 mouse lumbar motoneuron development. show that are initially expressed most newly motoneurons, but subsequently become restricted to lateral division motor column (lLMC). Disruption caused severe hindlimb locomotor defects. Motoneurons rostral segments were found adopt phenotype thoracic motoneurons. More caudally lLMC dorsal-projecting...
Aerial plant surfaces are covered by epicuticular waxes that among other purposes serve to control water loss. Maize glossy mutants originally identified their "glossy" phenotypes exhibit alterations in the accumulation of waxes. By combining data from a BSR-Seq experiment and newly developed Seq-Walking technology, GRMZM2G118243 was as strong candidate for being glossy13 gene. The finding multiple EMS-induced alleles contain premature stop codons GRMZM2G118243, one knockout allele gl13,...
The mechanisms responsible for the inhibitory effects of mesenchymal stem cells (MSCs) on dendritic (DCs) are still poorly understood. Our investigation potential signaling pathways revealed first time that human umbilical-cord-derived MSCs (UC-MSCs) instruct DCs to acquire tolerogenic phenotypes through IL-6-mediated upregulation SOCS1. This subset MSC-DCs exhibited a pattern, with clear decrease in expression co-stimulatory molecules and capacity stimulate CD3(+) T cell proliferation...
Cuticular waxes, long-chain hydrocarbon compounds, form the outermost layer of plant surfaces in most terrestrial plants. The presence cuticular waxes protects plants from water loss and other environmental stresses. Cloning characterization genes involved regulation, biosynthesis, extracellular transport onto surface epidermal cells have revealed molecular basis wax accumulation. However, intracellular trafficking synthesized to plasma membrane for cellular secretion is poorly understood....
Male sterility (MS) provides a useful breeding tool to harness hybrid vigor for seed production. It is necessary generate new male sterile mutant lines the development of production technology. The CRISPR/Cas9 technology well suited targeting genomes mutants. In this study, we artificially synthesized Streptococcus pyogenes Cas9 gene with biased codons maize. A vector MS8 maize was constructed and transformed into using an Agrobacterium-mediated method, eight T0 independent transgenic were...
Abstract Gene expression variation largely contributes to phenotypic diversity and constructing pan-transcriptome is considered necessary for species with complex genomes. However, the regulation mechanisms functional consequences of unexplored systematically. By analyzing RNA-seq data from 368 maize diverse inbred lines, we identified almost one-third nuclear genes under presence absence variation, which tend play regulatory roles are likely regulated by distant eQTLs. The ePAV was directly...
Summary Full‐length cDNAs are very important for genome annotation and functional analysis of genes. The number full‐length from maize ( Zea mays L.) remains limited. Here we report the construction a enriched cDNA library osmotically stressed seedlings by using modified CAP trapper method. From this library, 2073 (accession numbers DQ244142 – DQ246214 ) were collected further analyzed sequencing both 5′‐ 3′‐ends. A total 1728 (83.4%) sequences did not match known mRNA in GenBank database...