A5065848318- Congenital heart defects research
- Zebrafish Biomedical Research Applications
- Angiogenesis and VEGF in Cancer
- Coronary Artery Anomalies
- Congenital Heart Disease Studies
- Physiological and biochemical adaptations
- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- RNA modifications and cancer
- Proteoglycans and glycosaminoglycans research
- Pancreatic function and diabetes
- Cardiomyopathy and Myosin Studies
- Phytochemicals and Antioxidant Activities
- Signaling Pathways in Disease
- Protein Tyrosine Phosphatases
- Venomous Animal Envenomation and Studies
- Biomedical and Engineering Education
- Birth, Development, and Health
- Cell Adhesion Molecules Research
- MicroRNA in disease regulation
- Cardiac Valve Diseases and Treatments
- Nicotinic Acetylcholine Receptors Study
- Biochemical Analysis and Sensing Techniques
- Plant Reproductive Biology
- Plant Molecular Biology Research
University of Ioannina
2023-2025
Academy of Athens
2015-2024
Biomedical Research Foundation of the Academy of Athens
2019-2024
University of California, San Francisco
2003-2007
Olympic Athletic Center of Athens "Spiros Louis"
2006
Utrecht University
1999-2004
Tube and lumen formation are essential steps in forming a functional vasculature. Despite their significance, our understanding of these processes remains limited, especially at the cellular molecular levels. In this study, we analyze mechanisms angioblast coalescence zebrafish embryonic midline subsequent vascular tube formation. To facilitate studies, generated transgenic line where EGFP expression is controlled by flk1 promoter. We find that angioblasts migrate as individual cells to form...
Defects in cardiac valve morphogenesis and septation of the heart chambers constitute some most common human congenital abnormalities. Some these defects originate from errors atrioventricular (AV) endocardial cushion development. Although this process is being extensively studied mouse chick, zebrafish system presents several advantages over models, including ability to carry out forward genetic screens study vertebrate gene function at single cell level. In paper, we analyze cellular...
Abstract Venoms have evolved >100 times in all major animal groups, and their components, known as toxins, been fine-tuned over millions of years into highly effective biochemical weapons. There are many outstanding questions on the evolution toxin arsenals, such how venom genes originate, contributes to fitness venomous species, which modifications at genomic, transcriptomic, protein level drive evolution. These received particularly little attention outside snakes, cone snails,...
Vertebrate hearts depend on highly specialized cardiomyocytes that form the cardiac conduction system (CCS) to coordinate chamber contraction and drive blood efficiently unidirectionally throughout organism. Defects in this wiring can lead syncope sudden death. Thus, a greater understanding of development may help prevent these devastating clinical outcomes. Utilizing cardiac-specific fluorescent calcium indicator zebrafish transgenic line, Tg(cmlc2:gCaMP)s878, allows for vivo optical...
Valvular heart disease is responsible for considerable morbidity and mortality. Cardiac valves develop as the contracts, they function throughout lifetime of organism to prevent retrograde blood flow. Their precise morphogenesis crucial cardiac function. Zebrafish an ideal model investigate valve development it allows these studies be carried out in vivo through non-invasive imaging. Accumulating evidence suggests a role contractility intracardiac flow dynamics development. However, two...
In therapeutic interventions associated with melanin hyperpigmentation, tyrosinase is regarded as a target enzyme it catalyzes the rate-limiting steps in mammalian melanogenesis. Since many known agents have been proven to be toxic, there has increasing impetus identify alternative inhibitors, especially from natural sources. this study, we investigated 900 extracts Greek plants for potential inhibitive properties. Among five most potent extracts, methanol extract of Morus alba wood (MAM)...
Extracellular matrix (ECM) deposition after central nervous system (CNS) injury leads to inhibitory scarring in humans and other mammals, whereas it facilitates axon regeneration the zebrafish. However, molecular basis of these different fates is not understood. Here, we identify small leucine-rich proteoglycans (SLRPs) as a contributing factor failure mammals. We demonstrate that SLRPs chondroadherin, fibromodulin, lumican, prolargin are enriched rodent human but zebrafish CNS lesions....
Soluble guanylyl cyclase (sGC) is a cGMP-generating enzyme carrying heme prosthetic group that functions as nitric oxide (NO) sensor. sGC present in most cells types, including the vascular endothelium, where its biological remain largely unexplored. Herein, we have investigated role of angiogenesis and angiogenesis-related properties endothelial (EC). Initially, determined was enzymatically active chicken chorioallantoic membrane (CAM) during days maximal angiogenesis. In CAM, inhibition...
Protein tyrosine phosphatase receptor zeta 1 (PTPRZ1) is a transmembrane (TP) that serves as for pleiotrophin (PTN) and vascular endothelial growth factor A 165 (VEGFA
The current study reports on the toxicity, uptake, and biotransformation potential of zebrafish (embryos larvae) exposed to benzotriazoles (BTs). Acute toxicity assays were conducted. Cardiac function abnormalities (pericardial edema poor blood circulation) observed from phenotypic analysis early life embryos after BTs exposure. For uptake experiment, extracts whole body larvae analyzed using liquid chromatography-high-resolution tandem mass spectrometry (UPLC-Q-TOF-HRMS/MS). utility...