A5020615212- Antibiotic Resistance in Bacteria
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Cytomegalovirus and herpesvirus research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Antibiotics Pharmacokinetics and Efficacy
- Herpesvirus Infections and Treatments
- Respiratory viral infections research
- Influenza Virus Research Studies
- Pneumonia and Respiratory Infections
- X-ray Diffraction in Crystallography
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Crystallization and Solubility Studies
- Long-Term Effects of COVID-19
- Salmonella and Campylobacter epidemiology
- Microbial Natural Products and Biosynthesis
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Escherichia coli research studies
- Viral Infections and Immunology Research
- Cancer therapeutics and mechanisms
- Hepatitis B Virus Studies
- COVID-19 Impact on Reproduction
Hospital Universitario Virgen del Rocío
2014-2024
Instituto de Biomedicina de Sevilla
2015-2024
Universidad de Sevilla
2015-2024
Centro de Investigación Biomédica en Red
2022-2024
Instituto de Salud Carlos III
2020-2024
Junta de Andalucía
2024
Red Espanola de Investigacion en Patologia Infecciosa
2020
Scripps Research Institute
2014
Universitat de Barcelona
2003-2011
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2003-2010
Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity components. Dendritic cells (DCs) play a key role in the defense against viral infections, for instance plasmacytoid DCs (pDCs), have capacity to produce vast amounts of interferon-alpha (IFN-α). In COVID-19 there is deficit DC numbers and IFN-α production, which has been associated with disease severity. this work, we described that addition deficiency, several...
Abstract Venoms have evolved >100 times in all major animal groups, and their components, known as toxins, been fine-tuned over millions of years into highly effective biochemical weapons. There are many outstanding questions on the evolution toxin arsenals, such how venom genes originate, contributes to fitness venomous species, which modifications at genomic, transcriptomic, protein level drive evolution. These received particularly little attention outside snakes, cone snails,...
Abstract The main objective was to evaluate the viability of SARS-CoV-2 viral particles excreted in stools. In addition, we aimed identify clinical factors associated with detection RNA feces, and determine if its presence is an unfavorable outcome, defined as intensive care unit (ICU) admission and/or death. A prospective multicenter cohort study COVID-19 adult patients, confirmed infection by RT-PCR assay nasopharyngeal (NP) swabs admitted four hospitals Spain, from March 2020 February...
Abstract The repositioning of drugs already approved by regulatory agencies for other indications is an emerging alternative the development new antimicrobial therapies. process involves lower risks and costs than de novo novel drugs. Currently, infections adenovirus show a steady increment with high clinical impact in immunosuppressed immunocompetent patients. lack safe efficacious drug to treat these supports search antiviral Here we evaluated anti-adenovirus activity niclosanide,...
Several imipenem-susceptible and -resistant Aeromonas veronii biovar sobria isolates with different morphologies antimicrobial susceptibilities recovered from bile samples of a patient cholangitis were analysed. These belonged to the same clone imipenem-resistant strains showed overexpression imiS gene, encoding chromosomal carbapenemase. results should make clinicians aware possible emergence multidrug-resistant A. sobria, perhaps as consequence previous treatment urinary tract infection...
Abstract Multiple multicomponent reactions rapidly assemble complex structures. Despite being very productive, the lack of selectivity and reduced number viable transformations restrict their general application in synthesis. Hereby, we describe a rationale for selective version these processes based preferential generation intermediates which are less reactive than initial substrates. In this way, applying Groebke–Blackburn–Bienaymé reaction on range α‐polyamino‐polyazines, prepared family...
Abstract The aim was to assess the ability of nasopharyngeal SARS-CoV-2 viral load at first patient’s hospital evaluation predict unfavorable outcomes. We conducted a prospective cohort study including 321 adult patients with confirmed COVID-19 through RT-PCR in swabs. Quantitative Synthetic RNA cycle threshold values were used calculate log 10 copies/mL. Disease severity end follow up categorized into mild, moderate, and severe. Primary endpoint composite intensive care unit (ICU) admission...
We have studied the bacterial resistance to fluoroquinolones that arises as a result of mutations in DNA gyrase target protein. Although it is known quinolone antibacterial agents, molecular details quinolone-gyrase interaction remain unclear. The mode binding ciprofloxacin, levofloxacin, and moxifloxacin was analyzed by means docking calculations over surface QRDR GyrA. analysis these models allows study mechanism associated with gyrA more commonly found E. coli fluoroquinolone-resistant...
The role of sdiA in the acquisition low-level multidrug resistance (MDR) was analysed and compared with that marA soxS two Escherichia coli clinical isolates vitro-selected mutants. mutants were developed by growth lomefloxacin ceftazidime. sdiA, marA, soxS, ftsI, tolC acrB gene transcript levels determined RT–PCR. Analyses 2,4-dinitrophenol susceptibility, effect an active efflux inhibitor on antibiotic mitomycin C β-lactamase hydrolytic activity, outer inner membrane proteins acrR...
Abstract Background BPZE1, a live attenuated intranasal (IN) pertussis vaccine, is designed to prevent Bordetella (Bp) infection, disease and transmission address limitations of current vaccines. BPZE1 has been shown protect adults, primed with whole cell vaccine in infancy, from colonization virulent Bp. This study assessed BPZE1-induced immune responses, non-interference safety when administered without tetanus-diphtheria-acellular (Tdap; Boostrix™) children 6-17 years old, infancy...
The search for human adenovirus (HAdV)-specific antiviral drugs the treatment of HAdV infections in immunocompromised patients continues to be a challenging goal medicinal chemistry. Here, we report synthesis, biological evaluation, and structure-activity relationships small molecules library. We have identified six phenylpiperazine derivatives that significantly inhibited infection. These compounds showed capacity block and, addition, cytomegalovirus (HCMV) replications at low micromolar...
Hemagglutination-inhibitory antibodies are usually highly strain specific with little effect on infection drifted or shifted strains. The significance of broadly cross-reactive non-HAI anti-influenza against conserved domains virus glycoproteins, such as the hemagglutinin (HA) stalk, is great interest. We characterize a cohort 40 H1N1pmd09 influenza-infected patients and identify lower respiratory symptoms (LRSs) predictor for development pneumonia. A binomial logistic regression log10...
The effective treatment of adenovirus (HAdV) infections in immunocompromised patients still poses great challenges. Herein, we reported our continued efforts to optimize a series salicylamide derivatives as potent inhibitors HAdV infection. Of these, nine compounds (11, 13, 14, 17, 20, 58, 60, 62, and 70) showed significantly improved anti-HAdV activities with nanomolar submicromolar IC50 values high selectivity indexes (SI > 100), indicating better safety windows, compared those the lead...
The aim was to analyze the characteristics and predictors of unfavorable outcomes in solid organ transplant recipients (SOTRs) with COVID-19. We conducted a prospective observational cohort study 210 consecutive SOTRs hospitalized COVID-19 12 Spanish centers from 21 February 6 May 2020. Data pertaining demographics, chronic underlying diseases, transplantation features, clinical, therapeutics, complications were collected. primary endpoint composite intensive care unit (ICU) admission and/or...
Regardless of the clinical impact human adenovirus (HAdV) infections in healthy population and its high morbidity immunosuppressed patients, a specific treatment is still not yet available. In this study, we screened CM1407 COST Action's chemical library, comprising 1,233 natural products to identify compounds that restrict HAdV infection. Among them, identified rotenolone, compound significantly inhibited Next, selected four isoflavonoid-type (e.g., rotenone, deguelin, millettone,...
Human adenovirus (HAdV) and cytomegalovirus (HCMV) are cause of high morbidity mortality in patients receiving solid organ (SOT) hematopoietic stem cell transplantations (HSCT). Immunosuppressors universally used to prevent graft-versus-host disease (GVHD) HSCT graft rejection SOT. The long-term use these drugs is associated with a risk infections, but there also evidences their specific interference virus infection. antiviral activity immunosuppressors commonly the clinical practice SOT...
Despite the increasingly widespread clinical impact of adenovirus (HAdV) infections in healthy individuals and associated high morbidity immunosuppressed patients, particularly among paediatric population, a specific treatment for this virus has yet to be developed. In study, we report anti-HAdV activity sub-micromolar concentrations four heteroleptic (C^S)-cycloaurated complexes bearing single thiophosphinamide [