A5052890995- Venomous Animal Envenomation and Studies
- Rabies epidemiology and control
- Biochemical and Structural Characterization
- Marine Invertebrate Physiology and Ecology
- Antimicrobial Peptides and Activities
- Healthcare and Venom Research
- Yersinia bacterium, plague, ectoparasites research
- Insect and Pesticide Research
- Amphibian and Reptile Biology
- Marine Toxins and Detection Methods
- Microbial Metabolic Engineering and Bioproduction
- Plant Pathogenic Bacteria Studies
- Immunotherapy and Immune Responses
- Nicotinic Acetylcholine Receptors Study
- Veterinary Oncology Research
- Animal Ecology and Behavior Studies
- Neurobiology and Insect Physiology Research
- Invertebrate Immune Response Mechanisms
- Cellular transport and secretion
- Microbial Natural Products and Biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Bioactive Natural Diterpenoids Research
- Metabolomics and Mass Spectrometry Studies
- vaccines and immunoinformatics approaches
Freie Universität Berlin
2024-2025
Technische Universität Berlin
2016-2022
Charité - Universitätsmedizin Berlin
2021-2022
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2021
Abstract Venoms have evolved >100 times in all major animal groups, and their components, known as toxins, been fine-tuned over millions of years into highly effective biochemical weapons. There are many outstanding questions on the evolution toxin arsenals, such how venom genes originate, contributes to fitness venomous species, which modifications at genomic, transcriptomic, protein level drive evolution. These received particularly little attention outside snakes, cone snails,...
Genomics and metabolomics are widely used to explore specialized metabolite diversity. The Paired Omics Data Platform is a community initiative systematically document links between metabolome (meta)genome data, aiding identification of natural product biosynthetic origins structures.
Venom systems are key adaptations that have evolved throughout the tree of life and typically facilitate predation or defense. Despite venoms being model for studying a variety evolutionary physiological processes, many taxonomic groups remain understudied, including venomous mammals. Within order Eulipotyphla, multiple shrew species solenodons oral venom systems. morphological variation their delivery systems, it remains unclear whether represents ancestral state in this group is result...
The Nose-horned Viper (Vipera ammodytes) is one of the most widespread and venomous snakes in Europe, which causes high frequent snakebite accidents. first comprehensive venom characterization regional endemic Transcaucasian ammodytes transcaucasiana) Transdanubian Sand montandoni) reported employing a combination intact mass profiling bottom-up proteomics. analysis both subspecies identified major snake protein families viper venoms. Furthermore, revealed presence two tripeptidic...
Identifying individual age and size is crucial in venom research to understand potential phenotypic changes. When studying ontogeny, juveniles adults are often determined by at sexual maturity. However, gape-limited predators such as snakes, shifts may occur earlier, coinciding with an increase jaw that allows the intake of larger prey. This study explored variation Vipera ammodytes along snout-vent length (SVL), linking these changes dietary reproductive status. A total 57 samples from two...
Herein, we report on the venom proteome of Vipera anatolica senliki, a recently discovered and hitherto unexplored subspecies critically endangered Anatolian meadow viper endemic to Antalya Province Turkey. Integrative venomics, including gland transcriptomics as well complementary bottom-up top-down proteomics analyses, were applied fully characterize V. a. senliki. Furthermore, classical venomics approach was extended elucidate by an alternative in-source decay (ISD) workflow using...
The Asian world is home to a multitude of venomous and dangerous snakes, which are used induce various medical effects in the preparation traditional snake tinctures alcoholics, like Japanese wine, named Habushu. aim this work was perform first quantitative proteomic analysis Protobothrops flavoviridis pit viper venom. Accordingly, venom analyzed by complimentary bottom-up top-down mass spectrometry techniques. spectrometry-based venomics approach revealed that more than half composed...
Among venomous animals, toxic secretions have evolved as biochemical weapons associated with various highly specialized delivery systems on many occasions. Despite extensive research, there is still limited knowledge of the functional biology most animal toxins, including their venom production and storage, well morphological structures within sophisticated producing tissues that might underpin modulation. Here, we report spatial exploration a snake gland system by matrix-assisted laser...
Albicidin is a potent antibiotic and phytotoxin produced by Xanthomonas albilineans which targets the plant bacterial DNA gyrase. We now report on new albicidin derivative carbamoylated at N-terminal coumaric acid action of ATP-dependent O-carbamoyltransferase Alb15, present in (alb) gene cluster. Carbamoyl-albicidin was characterized tandem mass spectrometry from cultures overproducer strain function confirmed inactivation alb15 X. albilineans. Expression Escherichia coli vitro...
We report a novel hybrid, molecular and elemental mass spectrometry (MS) setup for the absolute quantification of snake venom proteomes shown here two desert black cobra species within genus Walterinnesia, Walterinnesia aegyptia morgani. The experimental design includes decomplexation samples by reverse-phase chromatography independently coupled to four systems: combined bottom-up top-down MS protein identification parallel microbore high-performance liquid chromatograph (RP-μHPLC) on-line...
Abstract Research on animal venoms and their components spans multiple disciplines, including biology, biochemistry, bioinformatics, pharmacology, medicine, more. Manipulating analyzing the diverse array of data required for venom research can be challenging, relevant tools resources are often dispersed across different online platforms, making them less accessible to nonexperts. In this article, we address multifaceted needs scientific community involved in toxin-related by identifying...
Assassin bug venoms are potent and exert diverse biological functions, making them potential biomedical goldmines. Besides feeding functions on arthropods, assassin bugs also use their venom for defense purposes causing localized systemic reactions in vertebrates. However, remain poorly characterized. We collected the from Rhynocoris iracundus investigated its composition bioactivity vitro vivo. It caused lysis of murine neuroblastoma, hepatoma cells, healthy myoblasts. demonstrated, first...
Abstract Background The identification of novel toxins from overlooked and taxonomically exceptional species bears potential for various pharmacological applications. remipede Xibalbanus tulumensis , an underwater cave-dwelling crustacean, is the only crustacean which a venom system has been described. Its contains several xibalbin peptides that have inhibitor cysteine knot (ICK) scaffold. Results Our screenings revealed all tested variants particularly inhibit potassium channels. Xib 1 xib...
Canine cutaneous histiocytoma (CCH) is a tumor that originates from dermal Langerhans cells and affects particularly young dogs. The common spontaneous regression of CCH makes it an interesting model in comparative oncology research. Previous studies have indicated anti-tumor immune responses may be involved, but details remain speculative to date. Here, we asked which specific immuno-oncological dynamics underlie on mRNA protein levels. QuantSeq 3′ sequencing with functional...
Snake venoms are extremely important for alternative medicine applications and discovery of new bioactive molecules. Especially in the recent years, there promising studies literature on development therapies targeting cancer with use snake venom peptides. In present study, PLA2 SVMPs peptide fractions M. bulgardaghica were investigated their apoptotic effects human cervix adenocarcinoma cell line, HeLa lung A549. The cytotoxicity crude was assessed by MTT assay IC50 values 4.34 ± 0.19 2.60...
The Asian world is home to a multitude of venomous and dangerous snakes, which are attributed various medical effects used in the preparation traditional snake tinctures alcoholics, like Japanese wine, named Habushu. aim this work was perform first quantitative proteomic analysis Protobothrops flavoviridis pit viper venom. Accordingly, venom analyzed by complimentary bottom-up top-down mass spectrometry techniques. spectrometry-based venomics approach revealed that more than half composed...
Abstract Among venomous animals, toxic secretions have evolved as biochemical weapons associated with various highly specialized delivery systems on many occasions. Despite extensive research, there is still limited knowledge of the functional biology most animal toxins, including their venom production and storage, well morphological structures within sophisticated producing tissues that might underpin modulation. Here we report spatial exploration a snake gland system by matrix-assisted...
Abstract Herein we report on the venom proteome of Vipera anatolica senliki, a recently discovered and hitherto unexplored subspecies critically endangered Anatolian Meadow viper endemic to Antalya Province Turkey. Integrative venomics, including gland transcriptomics as well complementary bottom-up top-down proteomic analyses, were applied fully characterize V. a. senliki . Furthermore, classical venomics approach was extended elucidate by an alternative in-source decay (ISD) proteomics...
Abstract Animal secretions are of great interest in terms drug development due to their complex protein and peptide composition. Especially, the field therapeutic medications such as anti-cancer drugs snake venoms receive attention. In this study we report two Viperidae species from various habitats with a particular focus on cytotoxic potential along decomplexation venom proteome: horned desert viper ( Cerastes cerastes ), native regions North Africa mangrove pit (Cryptelytrops...
Abstract We report on the variable venom composition of a population Caucasus viper ( Vipera kaznakovi ) in Northeastern Turkey. applied combination gland transcriptomics, as well de-complexing bottom-up and top-down venomics, enabling comparison proteomes from multiple individuals. In total, we identified peptides proteins 15 toxin families, including snake metalloproteinases (svMP; 37.8%), phospholipases A 2 (PLA ; 19.0%), serine proteinases (svSP; 11.5%), C-type lectins (CTL; 6.9%)...