A5022203306- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Probiotics and Fermented Foods
- Microbial Community Ecology and Physiology
- Viral gastroenteritis research and epidemiology
- Fatty Acid Research and Health
- Intracerebral and Subarachnoid Hemorrhage Research
- Infant Nutrition and Health
- Microbial Metabolic Engineering and Bioproduction
- Clinical Nutrition and Gastroenterology
- Microbial Metabolism and Applications
- Neonatal and fetal brain pathology
- Psychological and Temporal Perspectives Research
- Intensive Care Unit Cognitive Disorders
- Intracranial Aneurysms: Treatment and Complications
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Insect symbiosis and bacterial influences
- Mycorrhizal Fungi and Plant Interactions
- Peripheral Neuropathies and Disorders
- Myasthenia Gravis and Thymoma
- Neurofibromatosis and Schwannoma Cases
University of Chicago
2022-2025
University of Illinois Chicago
2019-2023
Despite rapid evolution in the area of microbial natural products chemistry, there is currently no open access database containing all microbially produced product structures. Lack availability these data preventing implementation new technologies science. Specifically, development computational strategies for compound characterization and identification are being hampered by lack a comprehensive known compounds against which to compare experimental data. The creation an access,...
Genomics and metabolomics are widely used to explore specialized metabolite diversity. The Paired Omics Data Platform is a community initiative systematically document links between metabolome (meta)genome data, aiding identification of natural product biosynthetic origins structures.
Abstract Respiratory failure and mortality from COVID-19 result virus- inflammation-induced lung tissue damage. The intestinal microbiome associated metabolites are implicated in immune responses to respiratory viral infections, however their impact on progression of severe remains unclear. We prospectively enrolled 71 patients with critical illness, collected fecal specimens within 3 days medical intensive care unit admission, defined compositions by shotgun metagenomic sequencing,...
Maternal immune activation (MIA) derived from late gestational infection such as seen in chorioamnionitis poses a significantly increased risk for neurodevelopmental deficits the offspring. Manipulating early microbiota through maternal probiotic supplementation has been shown to be an effective means improve outcomes; however, mechanisms remain unclear. In this study, we demonstrated that MIA modeled by exposing pregnant dams lipopolysaccharide (LPS) induced underdevelopment of blood...
Increases in mean lesional iron content by quantitative susceptibility mapping (QSM) ≥6% and/or vascular permeability dynamic contrast enhanced perfusion (DCEQP) ≥40% on MRI have been associated with new symptomatic hemorrhage (SH) cerebral cavernous malformations (CCMs). It is not known if plasma biomarkers can reflect these changes within the lesion proper. This cohort study enrolled 46 CCM patients SH prior year. Plasma samples, QSM and DCEQP were simultaneously acquired at beginning end...
Species of the Bacteroidales order are among most abundant and stable bacterial members human gut microbiome with diverse impacts on health. While strains species genomically functionally diverse, order-wide comparative analyses lacking. We cultured sequenced genomes 408 isolates from healthy donors representing nine genera 35 performed genomic, gene-specific, mobile gene, metabolomic analyses. Families, genera, could be grouped based many distinctive features. However, we also show...
Abstract Clostridioides difficile (C. difficile) strains belonging to the epidemic BI/NAP1/027 (RT027) group have been associated with increased transmissibility and disease severity. In addition major toxin A B virulence factors, RT027 also encode CDT binary toxin. Our lab previously identified a toxigenic isolate, ST1-75, that is avirulent in mice despite densely colonizing colon. Here, we show co-infecting ST1-75 virulent R20291 protects from colitis due rapid clearance of strain...
Bacterial–fungal interactions (BFIs) were explored in pairwise co-cultures of species from the cheese rind microbiome, revealing that fungi are key contributors to small molecule mediate BFIs and important microbiomes.
ABSTRACT Microbial interactions are major determinants in shaping microbiome structure and function. Although fungi found across diverse microbiomes, the mechanisms through which interact with other species remain largely uncharacterized. In this work, we explore diversity of ways can impact bacteria by characterizing interaction 16 different bacterial-fungal pairs, involving 8 2 ( Escherichia coli Pseudomonas psychrophila ). Using random barcode transposon-site sequencing (RB-TnSeq),...
Bacterial-fungal interactions (BFIs) can shape the structure of microbial communities, but small molecules mediating these BFIs are often understudied. We explored various optimization steps for our culture and chemical extraction protocols bacterial-fungal co-cultures, liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that metabolomic profiles mainly comprised fungi derived features, indicating key contributors to molecule mediated BFIs. LC-inductively coupled plasma MS...
ABSTRACT The intestinal microbiome is composed of myriad microbial species that produce metabolites impact host health. While loss bacterial and beneficial from the fecal associated with development a range diseases medical complications, there are currently no diagnostic tests rapidly identify individuals deficiencies. Reduced concentrations butyrate deoxycholic acid adverse clinical outcomes result subset health-associated species. We present rapid test based on 3-nitrophenylhydrazine...
Background: Increase in mean lesional iron content (≥6%) measurement by QSM and vascular permeability (≥ 40%) assessed DCEQP MRI have been associated with new bleeding cavernous angiomas (CA), are used as monitoring biomarkers clinical trial of pharmacotherapy CAs symptomatic hemorrhage (SH). Plasma metabolites CA unsupervised discovery, linked mechanistically to the permissive microbiome angiogenic inflammatory mechanisms CAs. To date, their levels change has not compared during prospective...