A5039582994- Vascular Malformations Diagnosis and Treatment
- Intracerebral and Subarachnoid Hemorrhage Research
- Intracranial Aneurysms: Treatment and Complications
- MRI in cancer diagnosis
- Glioma Diagnosis and Treatment
- Moyamoya disease diagnosis and treatment
- Acute Ischemic Stroke Management
- Radiomics and Machine Learning in Medical Imaging
- Barrier Structure and Function Studies
- Cerebrovascular and genetic disorders
- Vascular Malformations and Hemangiomas
- Extracellular vesicles in disease
- Treatment of Major Depression
- Retinal and Macular Surgery
- Domain Adaptation and Few-Shot Learning
- Optical Imaging and Spectroscopy Techniques
- Functional Brain Connectivity Studies
- Advanced MRI Techniques and Applications
- Brain Tumor Detection and Classification
University of Chicago
2019-2025
Neurological Surgery
2021-2025
Increases in mean lesional iron content by quantitative susceptibility mapping (QSM) ≥6% and/or vascular permeability dynamic contrast enhanced perfusion (DCEQP) ≥40% on MRI have been associated with new symptomatic hemorrhage (SH) cerebral cavernous malformations (CCMs). It is not known if plasma biomarkers can reflect these changes within the lesion proper. This cohort study enrolled 46 CCM patients SH prior year. Plasma samples, QSM and DCEQP were simultaneously acquired at beginning end...
The purpose of this study was to determine important genes, functions, and networks contributing the pathobiology cerebral cavernous malformation (CCM) from transcriptomic analyses across 3 species 2 disease genotypes. Sequencing RNA laser microdissected neurovascular units 5 human surgically resected CCM lesions, mouse brain microvascular endothelial cells, Caenorhabditis elegans with induced Ccm gene loss, their respective controls provided differentially expressed genes (DEGs). DEGs C....
BACKGROUNDCerebral cavernous angiomas (CAs) with a symptomatic hemorrhage (CASH) have high risk of recurrent and serious morbidity.METHODSEighteen plasma molecules mechanistic roles in CA pathobiology were investigated 114 patients 12 healthy subjects. The diagnostic biomarker CASH the prior year was derived as that minimizing Akaike information criterion validated using machine learning, compared prognostic predicting bleeding subsequent year. Biomarkers longitudinally followed subset...
Cerebral cavernous malformations (CCMs) are dilated capillaries causing epilepsy and stroke. Inheritance of a heterozygous mutation in CCM3/PDCD10 is responsible for the most aggressive familial form disease. Here we studied differences commonalities between transcriptomes microdissected lesional neurovascular units (NVUs) from acute chronic vivo Ccm3/Pdcd10ECKO mice, cultured brain microvascular endothelial cells (BMECs) Ccm3/Pdcd10ECKO.We identified 2409 differentially expressed genes...
Abstract Cavernous angiomas (CA) are common vascular anomalies causing brain hemorrhage. Based on mouse studies, roles of gram-negative bacteria and altered intestinal homeostasis have been implicated in CA pathogenesis, pilot study had suggested potential microbiome differences between non-CA individuals based 16S rRNA gene sequencing. We here assess a larger cohort human subjects with without CA, among different clinical features, conduct more definitive microbial analyses using...
Introduction: Familial cerebral cavernous malformations (FCCM), Sturge-Weber Syndrome (SWS), and hereditary hemorrhagic telangiectasia with brain arteriovenous (HHT) are neurovascular disorders driven by genetic mutations while microbleeds (CMBs) primarily associated the aging process. All different vascular dysmorphisms and/or propensity to bleed. Hypothesis: We hypothesized that common distinct circulating microribonucleic acids (miRNAs), reflecting shared pathobiology, can serve as...
Cerebral cavernous angioma (CA) is a capillary microangiopathy predisposing more than million Americans to premature risk of brain hemorrhage. CA with recent symptomatic hemorrhage (SH), most likely re-bleed serious clinical sequelae, the primary focus therapeutic development. Signaling aberrations in include proliferative dysangiogenesis, blood-brain barrier hyperpermeability, inflammatory/immune processes, and anticoagulant vascular domain. Plasma levels molecules reflecting these...
Abstract Background: Cavernous angiomas (CAs) affect 0.5% of the population, predisposing to serious neurologic sequelae from brain bleeding. A leaky gut epithelium associated with a permissive microbiome, was identified in patients who develop CAs, favoring lipid polysaccharide producing bacterial species. Micro-ribonucleic acids along plasma levels proteins reflecting angiogenesis and inflammation were also previously correlated CA symptomatic hemorrhage. Methods: The metabolome hemorrhage...
Abstract Cerebral cavernous malformation (CCM) is a hemorrhagic neurovascular disease with no currently available therapeutics. Prior evidence suggests that different cell types may play role in CCM pathogenesis. The contribution of each type to the dysfunctional cellular crosstalk remains unclear. Herein, RNA-seq was performed on fluorescence-activated sorted endothelial cells (ECs), pericytes, and neuroglia from lesions non-lesional brain tissue controls. Differentially Expressed Gene...
The purpose of this study was to systematically assess asymptomatic changes (ACs), including subclinical hemorrhage, growth, or new lesion formation (NLF) during longitudinal follow-up cerebral cavernous angiomas (CAs), and correlate these with symptomatic hemorrhage (SH) the same period clinical features disease.
Cerebral cavernous angioma (CA) is a capillary vasculopathy affecting more than million Americans with small fraction of cases demonstrating lesional bleed or growth major clinical sequelae. Perfusion and permeability are fundamental features CA pathophysiology, but their role as prognostic biomarkers unclear.
Cavernous angiomas with symptomatic hemorrhage (CASH) have a high risk of rebleeding, and hence an accurate diagnosis is needed. With blood flow vascular leak as established mechanisms, we analyzed perfusion permeability derivations dynamic contrast-enhanced quantitative (DCEQP) MRI in 745 lesions 205 consecutive patients. Thirteen respective lesional were compared between that bled within year prior to imaging (N = 86), versus non-CASH 659) using machine learning univariate analyses. Based...
Girard, Romuald PhD; Li, Yan Stadnik, Agnieszka MS; Shenkar, Robert Hobson, Nicholas Romanos, Sharbel BA; Srinath, Abhinav Moore, Thomas BS; Lightle, Rhonda Shkoukani, Abdallah MD; Akers, Amy Carroll, Timothy Christoforidis, Gregory A Koenig, James I Lee, Cornelia PsyD; Piedad, Kristina RN; Greenberg, Steven M MD/PhD; Kim, Helen Flemming, Kelly D Ji, Yuan Awad, Issam MD, MSc, MA (hon)
Background: Cerebral cavernous malformation is a common lesion affecting more than million Americans, with potential severe sequelae. It unclear how socioeconomic status (SES) and race influence access to specialized care, the enrollment adherence in clinical trials aimed at novel therapies for CCM. Methods: We analyzed cohort of patients harboring CCM lesions screened IRB approved research studies between 01/2010 -12/2021 an accredited center excellence CCM, leading NIH funded studies....
Background: Increases in mean lesional iron content (≥6%) measurement by QSM and vascular permeability (≥40%) assessed DCEQP MRI have been associated with new bleeding, are used as monitoring biomarkers NINDS funded 1st clinical trial of pharmacotherapy (clinicaltrials.gov NCT02603328) cavernous angiomas symptomatic hemorrhage (CASH). Plasma protein levels previously CASH diagnostic prognostic contexts never compared to the same subjects. Methods: samples sequences were simultaneously...