A5027395645- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Atherosclerosis and Cardiovascular Diseases
- Cell Image Analysis Techniques
- Renal Diseases and Glomerulopathies
- Immunodeficiency and Autoimmune Disorders
- Image Processing Techniques and Applications
- CAR-T cell therapy research
- Renal Transplantation Outcomes and Treatments
- Single-cell and spatial transcriptomics
- Immune Response and Inflammation
- Genomics and Chromatin Dynamics
- Chronic Lymphocytic Leukemia Research
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Vascular Malformations Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- NF-κB Signaling Pathways
- Epigenetics and DNA Methylation
- Blood groups and transfusion
University of Chicago
2015-2024
Boston Children's Hospital
2024
Boston Children's Museum
2024
University of Richmond
2014
Clark Art Institute
2014
Marcus (United States)
2014
University of Illinois Chicago
2013
University of Nebraska Medical Center
2010
National Institute of Pathology
2007
Center for Rheumatology
1998-2007
The most prevalent severe manifestation of systemic lupus erythematosus is nephritis, which characterized by immune complex deposition, inflammation, and scarring in glomeruli the tubulointerstitium. Numerous studies indicated that glomerulonephritis results from a break B cell tolerance, resulting local deposition complexes containing Abs reactive with ubiquitous self-Ags. However, pathogenesis tubulointerstitial disease not known. In this article, we demonstrate more than half cohort 68...
In lupus nephritis, glomerular injury correlates poorly with progression to renal failure. While the tubulointerstitium is also commonly involved, importance of such involvement not well defined. Therefore, we developed a simple method assess prognostic utility measuring tubulointerstitial inflammation (TI).Sixty-eight systemic erythematosus patients nephritis were enrolled. Tubulointerstitial lymphocytic infiltrates quantitated both by anti-CD45 antibody staining and standard histochemical...
T follicular helper (TFH) cells are critical for B cell activation in germinal centers and often observed human inflamed tissue. However, it is difficult to know if they contribute situ inflammation. Expressed markers define TFH subsets associated with distinct functions vitro. such may not reflect function. The delivery of help requires direct cognate recognition. We hypothesized that by visualizing quantifying interactions, we could directly assess competency situ. Therefore, developed...
The B cell antigen receptor complex is a hetero-oligomeric structure composed of binding, membrane immunoglobulin, and transducer-transporter substructures. substructure disulfide-linked dimers immunoglobulin (Ig)-α Ig-β/γ subunits that are products the mb-1(α) B29 (β/γ) genes. Although associates with Src family kinases activated after ligation, site interaction these other cytoplasmic effector molecules unknown. tails Ig-α Ig-β chains were found to associate distinct sets molecules. chain...
Anti-CD3 monoclonal antibodies (mAbs) are potent immunosuppressive agents used in clinical transplantation. However, the activation-related adverse side effects associated with these mAbs have prompted development of less toxic nonmitogenic anti-CD3 mAb therapies. At present, functional and biochemical consequences T cell exposure to is unclear. In this study, we examined early signaling events triggered by a mAb. Like mitogenic mAb, changes receptor (TCR) complex, including ζ chain tyrosine...
Two polymorphic forms of Fc receptor III (FcR III) are expressed on human neutrophils. These differ with respect to their apparent molecular masses after digestion N-glycanase, and reactivity MAb Gran 11 alloantisera which recognize determinants (NA1 NA2) the biallelic neutrophil antigen (NA) system. To determine basis for this polymorphism we isolated RNA from neutrophils NA1NA1 NA2NA2 homozygotes synthesized corresponding cDNAs. cDNAs encoding FcR were then amplified using polymerase chain...
Pre-B-cell expansion is driven by signals from the interleukin-7 receptor and pre-B-cell dependent on cyclin D3 c-Myc. We have shown previously that interferon regulatory factors 4 8 induce expression of Ikaros Aiolos to suppress proliferation. However, molecular mechanisms through which exert their growth inhibitory effect remain be determined. Here, we provide evidence bind c-Myc promoter in vivo directly pre-B cells. further show downregulation critical for growth-inhibitory Aiolos. also...
The RNA N6-methyladenosine (m6A) methylation is installed by the METTL3-METTL14 methyltransferase complex. This modification has critical regulatory roles in various biological processes. Here, we report that deletion of Mettl14 dramatically reduces mRNA m6A developing B cells and severely blocks cell development mice. Deletion impairs interleukin-7 (IL-7)-induced pro-B proliferation large-pre-B-to-small-pre-B transition causes dramatic abnormalities gene expression programs important for...
HIF-1 dictates effector function of infiltrating T cells in lupus, causing tissue damage that can be abrogated by its blockade murine models.
Engagement of the B-cell antigen receptor complex induces immediate activation receptor-associated Src family tyrosine kinases including p55blk, p59fyn, p53/56lyn, and perhaps p56lck, this response is accompanied by phosphorylation distinct cellular substrates. These act directly or indirectly to phosphorylate and/or activate effector proteins p42 (microtubule-associated protein kinase) (MAPK), phospholipases C-gamma 1 (PLC gamma 1) 2 2), phosphatidylinositol 3-kinase (PI 3-K),...
IgG Fc receptor II (Fc gamma RII) on human monocytes is polymorphic with respect to its appearance gels after isoelectric focusing and ability mediate T lymphocyte proliferation induced by murine anti-CD3 mAb of the IgG1 isotype (i.e., bind IgG1). To determine molecular basis for this polymorphism, we isolated total cellular RNA from PBMC responders nonresponders (defined Leu-4-induced [3H] thymidine incorporation) synthesized corresponding cDNA. Sequences encoding extracellular domain RII...