A5064934512- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Protease and Inhibitor Mechanisms
- Genomics and Chromatin Dynamics
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Blood Coagulation and Thrombosis Mechanisms
- Epigenetics and DNA Methylation
- Reproductive System and Pregnancy
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- Ubiquitin and proteasome pathways
- Plant Pathogens and Fungal Diseases
- Signaling Pathways in Disease
- Chromatin Remodeling and Cancer
- Cancer-related Molecular Pathways
- Studies on Chitinases and Chitosanases
- Pregnancy and Medication Impact
- Nitric Oxide and Endothelin Effects
- RNA Research and Splicing
- Fungal Biology and Applications
- Preterm Birth and Chorioamnionitis
- Biopolymer Synthesis and Applications
- IL-33, ST2, and ILC Pathways
University of Chicago
2012-2024
University of Illinois Chicago
2013
Government of West Bengal
2013
University of Nebraska Medical Center
2010
University of Kalyani
1999-2004
Indian Agricultural Research Institute
2000-2002
Rosalind Franklin University of Medicine and Science
1995
Pre-B-cell expansion is driven by signals from the interleukin-7 receptor and pre-B-cell dependent on cyclin D3 c-Myc. We have shown previously that interferon regulatory factors 4 8 induce expression of Ikaros Aiolos to suppress proliferation. However, molecular mechanisms through which exert their growth inhibitory effect remain be determined. Here, we provide evidence bind c-Myc promoter in vivo directly pre-B cells. further show downregulation critical for growth-inhibitory Aiolos. also...
The RNA N6-methyladenosine (m6A) methylation is installed by the METTL3-METTL14 methyltransferase complex. This modification has critical regulatory roles in various biological processes. Here, we report that deletion of Mettl14 dramatically reduces mRNA m6A developing B cells and severely blocks cell development mice. Deletion impairs interleukin-7 (IL-7)-induced pro-B proliferation large-pre-B-to-small-pre-B transition causes dramatic abnormalities gene expression programs important for...
The pre–T cell receptor (TCR) is expressed early during T development and imposes a tight selection for differentiating progenitors. Pre-TCR–expressing cells are selected to survive differentiate further, whereas pre-TCR− “negatively” die. mechanisms of pre-TCR–mediated survival poorly understood. Here, we describe the induction antiapoptotic gene BCL2A1 (A1) as potential mechanism regulating inhibition death. We characterize in detail signaling pathway involved A1 show that expression can...
Abstract B-1a cells are long-lived, self-renewing innate-like B that predominantly inhabit the peritoneal and pleural cavities. In contrast to conventional B-2 cells, have a receptor repertoire is biased towards bacterial self-antigens, promoting rapid response infection clearing of apoptotic cells. Although known primarily originate from fetal tissues, mechanisms by which they arise has been topic debate for many years. Here we show in liver versus bone marrow environment, reduced...
Zbtb16-encoded PLZF is a signature transcription factor (TF) that directs the acquisition of T-helper effector programs during development multiple innate lymphocyte lineages, including natural killer T cell, lymphoid mucosal-associated invariant cell and γδ lineages. also essential in osteoblast spermatogonial development. How Zbtb16 itself regulated different lineages incompletely understood. Here, by systematic CRISPR/Cas9-assisted deletions chromatin accessible regions within locus...
Germinal centers (GCs), sites of antibody affinity maturation, are organized into dark (DZ) and light (LZ) zones. Here, we show a B cell-intrinsic role for signal transducer activator transcription 3 (STAT3) in GC DZ LZ organization. Altered zonal organization STAT3-deficient GCs dampens development long-lived plasma cells (LL-PCs) but increases memory (MBCs). In an abundant antigenic environment, achieved here by prime-boost immunization, STAT3 is not required initiation, maintenance, or...
Abstract The histone methyltransferase EZH2 is required for B and T cell development; however, the molecular mechanisms underlying this requirement remain elusive. In a murine model of lymphoid-specific deficiency we found that was proper development adaptive, but not innate, lymphoid cells. adaptive cells prevented premature expression Cdkn2a consequent stabilization p53, an effector pre–Ag receptor checkpoints. Deletion in EZH2-deficient lymphocytes p53 stabilization, extended lymphocyte...
Abstract Transcription factor (TF) networks determine cell fate in hematopoiesis. However, how TFs cooperate with other regulatory mechanisms to instruct transcription remains poorly understood. Here we show that small pre-B cells, the lineage restricted epigenetic reader BRWD1 closes early development enhancers and opens of late B lymphopoiesis TF binding. regulates over 7000 genes repress proliferative induce differentiation programs. does not regulate expression required for...
Immunoreceptor gene recombination requires complementary 12 bp and 23 signal sequences (RSSs). In addition, the RSSs that assemble RAG proteins, centers, must be accessible yet flanked by a 5-prime nucleosome decorated with H3K4me3. Drosophila, DNA GAGA motifs play an important role in positioning. Herein, we report to each functional Jk RSS is motif conserved across mammalian species. mice, Jk1 regulated local accessibility placement. Furthermore, it was required for Vk-Jk1 recombination....