A5023609359- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Chronic Lymphocytic Leukemia Research
- Neuroblastoma Research and Treatments
- Diabetes and associated disorders
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 and COVID-19 Research
- Immunodeficiency and Autoimmune Disorders
- COVID-19 Clinical Research Studies
- Renal Transplantation Outcomes and Treatments
- Pancreatic function and diabetes
- Glycosylation and Glycoproteins Research
- Blood groups and transfusion
- Cytomegalovirus and herpesvirus research
- Systemic Lupus Erythematosus Research
- vaccines and immunoinformatics approaches
- Single-cell and spatial transcriptomics
- Influenza Virus Research Studies
- Hematopoietic Stem Cell Transplantation
- Lymphoma Diagnosis and Treatment
- Immune Response and Inflammation
- Diabetes Management and Research
University of Pennsylvania
2016-2025
California University of Pennsylvania
2022-2025
Immune Regulation (United Kingdom)
2024
Hospital of the University of Pennsylvania
2003-2023
Translational Therapeutics (United States)
2023
The Wistar Institute
2016
Abramson Cancer Center
2016
Children's Hospital of Philadelphia
2012
Dana-Farber Cancer Institute
2012
Philadelphia University
2011
Immune memory after vaccination Vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has proven highly effective at preventing COVID-19. However, the evolution of viral variants, and waning antibody levels over time, raise questions regarding longevity vaccine-induced immune protection. Goel et al . examined B T lymphocyte responses in individuals who received SARS-CoV-2 messenger RNA vaccines. They performed a 6-month longitudinal study never had infection...
Although critical illness has been associated with SARS-CoV-2-induced hyperinflammation, the immune correlates of severe COVID-19 remain unclear. Here, we comprehensively analyzed peripheral blood perturbations in 42 SARS-CoV-2 infected and recovered individuals. We identified extensive induction activation multiple lineages, including T cell activation, oligoclonal plasmablast expansion, Fc trafficking receptor modulation on innate lymphocytes granulocytes, that distinguished cases from...
Novel mRNA vaccines for SARS-CoV-2 have been authorized emergency use. Despite their efficacy in clinical trials, data on vaccine-induced immune responses are mostly limited to serological analyses. Here, we interrogated antibody and antigen-specific memory B cells over time 33 naïve 11 recovered subjects. individuals required both vaccine doses optimal increases antibodies, particularly neutralizing titers against the B.1.351 variant. Memory specific full-length spike protein receptor...
SARS-CoV-2 messenger RNA vaccination in healthy individuals generates immune protection against COVID-19. However, little is known about mRNA vaccine-induced responses immunosuppressed patients. We investigated induction of antigen-specific antibody, B cell and T longitudinally patients with multiple sclerosis (MS) on anti-CD20 antibody monotherapy (n = 20) compared controls 10) after BNT162b2 or mRNA-1273 vaccination. Treatment monoclonal (aCD20) significantly reduced spike-specific...
COVID-19 is associated with a wide range of clinical manifestations, including autoimmune features and autoantibody production. Here we develop three protein arrays to measure IgG autoantibodies connective tissue diseases, anti-cytokine antibodies, anti-viral antibody responses in serum from 147 hospitalized patients. Autoantibodies are identified approximately 50% patients but less than 15% healthy controls. When present, largely target autoantigens rare disorders such as myositis, systemic...
Tisagenlecleucel, a chimeric antigen receptor (CAR) T-cell product targeting CD19 is approved for relapsed/refractory B-cell acute lymphoblastic leukemia (B-ALL). However, the impact of pretreatment variables, such as expression level, on leukemic blasts, presence CD19- subpopulations, and especially prior CD19-targeted therapy, response to CAR therapy has not been determined. We analyzed 166 patients treated with at our institution. Eleven did achieve minimal residual disease (MRD)- deep...
To study the effects in systemic lupus erythaematosus (SLE) of B cell directed therapy with rituximab, a chimeric monoclonal antibody at CD20+ cells, without concomitant immunosuppressive mild to moderate SLE.Patients (n=24) active SLE and failure >or=1 were recruited from three university centres into this phase I/II prospective open-label study. Patients followed for 1 year assess safety, efficacy biological effects.In total, 18 patients scheduled receive full lymphoma dose rituximab...
A functional B cell antigen receptor is thought to regulate antibody gene rearrangement either by stopping further (exclusion) or promoting additional (editing). We have developed a new model study the regulation of rearrangement. In this model, we used targeting replace J kappa region with V kappa-J light chain gene. Two different strains mice were created; one, 4R, has 4-J 4 followed downstream 5 segment, while other, 8R, 8-J chain. Here, analyze influence these chains on show that some 4R...
The PTPN22 genetic variant 1858T, encoding Lyp620W, is associated with multiple autoimmune disorders for which the production of autoantibodies a common feature, suggesting loss B cell tolerance. Lyp620W results in blunted BCR signaling memory cells. Because signal strength tightly coupled to central and peripheral tolerance, we examined whether impacts homeostasis healthy individuals heterozygous PTPN221858T variant. We found that these subjects display alterations composition pool include...
To study the safety and clinical efficacy of rituximab therapy for primary Sjögren's syndrome, as well to investigate its mechanisms.Patients with syndrome were enrolled in an open-label trial, given (1 gm) infusions on days 1 15, monitored through week 52. The end point was safety, secondary points evaluating biologic efficacy. Blood obtained enumeration lymphocyte subsets, measurement serum autoantibody BAFF levels, analysis gene expression.Twelve female patients administered rituximab....
High-throughput sequencing (HTS) of immunoglobulin (B-cell receptor, antibody) and T-cell receptor repertoires has increased dramatically since the technique was introduced in 2009 [1-3]. This experimental approach explores maturation adaptive immune system its response to antigens, pathogens disease conditions exquisite detail. It holds significant promise for diagnostic therapy-guiding applications. New technology often spreads rapidly, sometimes more rapidly than understanding how make...
Abstract Differences in immune responses to viruses and autoimmune diseases such as systemic lupus erythematosus (SLE) can show sexual dimorphism. Age-associated B cells (ABC) are a population of CD11c + T-bet critical for antiviral disorders. Absence DEF6 SWAP-70, two homologous guanine exchange factors, double-knock-out (DKO) mice leads lupus-like syndrome females marked by accumulation ABCs. Here we demonstrate that DKO ABCs sex-specific differences cell number, upregulation an ISG...