A5041632374- Viral Infections and Vectors
- Virology and Viral Diseases
- Rabies epidemiology and control
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- HER2/EGFR in Cancer Research
- Renal and related cancers
- Genetic and Kidney Cyst Diseases
- Viral Infections and Outbreaks Research
- Pediatric Urology and Nephrology Studies
- interferon and immune responses
- Digital Imaging in Medicine
- Gender, Security, and Conflict
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Neuroscience and Neuropharmacology Research
- Monoclonal and Polyclonal Antibodies Research
- Cancer Treatment and Pharmacology
- Olfactory and Sensory Function Studies
- Historical Gender and Feminism Studies
- Gut microbiota and health
- Clinical practice guidelines implementation
- Microbial Natural Products and Biosynthesis
University of Pennsylvania
2021-2025
Science and Technology Facilities Council
2021-2025
UK Research and Innovation
2024-2025
Rutherford Appleton Laboratory
2023
Thomas Jefferson University
2014-2020
University College London
2016
London School of Hygiene & Tropical Medicine
2010
University of Sheffield
1993
Therapies targeting blood vessels hold promise for autosomal dominant polycystic kidney disease (ADPKD), the commonest inherited disorder causing failure. However, onset and nature of vascular abnormalities in ADPKD are poorly defined. Accordingly, we employed a combination single-cell transcriptomics, three-dimensional imaging with geometric, topological fractal analyses, multimodal magnetic resonance arterial spin labelling to investigate aberrant microvasculature kidneys. Within human...
ABSTRACT Budding of filoviruses, arenaviruses, and rhabdoviruses is facilitated by subversion host proteins, such as Nedd4 E3 ubiquitin ligase, viral PPxY late (L) budding domains expressed within the matrix proteins these RNA viruses. As L are important for highly conserved in a wide array viruses, they represent potential broad-spectrum targets development antiviral drugs. To identify competitive blockers, we used known WW domain-PPxY interaction interface basis an silico screen. Using...
infection (CDI) is an urgent public health threat with limited preventative options. In this work, we developed a messenger RNA (mRNA)-lipid nanoparticle (LNP) vaccine targeting
Noninvasive visualization of the distribution and persistence mRNA vaccine antigen expression in mammalian systems has implications for development evaluation future vaccines. Here, we genetically fuse E. coli dihydrofolate reductase (eDHFR) to delta furin diproline modified SARS-CoV-2 spike glycoprotein (S2P∆f) image its female mice male non-human primates using [18F]fluoropropyl-trimethoprim ([18F]FP-TMP). Whole body positron emission tomography (PET) imaging revealed transient injection...
Understanding the interactions between rabies virus (RABV) and individual host cell proteins is critical for development of targeted therapies. Here we report that interferon-induced protein with tetratricopeptide repeats 2 (Ifit2), an interferon-stimulated gene (ISG) possible RNA-binding capacity, important restriction factor virus. When Ifit2 was depleted, RABV grew more quickly in mouse neuroblastoma cells vitro This effect replicated vivo, where knockout mice displayed a dramatically...
ABSTRACT Hallmarks of autosomal dominant polycystic kidney disease (ADPKD), the most common hereditary anomaly, include expanding fluid-filled epithelial cysts, inflammation, and fibrosis. Despite previous work showing potential vascular-based therapies, renal microvascular alterations in ADPKD, their timing, are poorly understood. Using single-cell transcriptomics human microvasculature, we identify a population endothelial cells adjacent to cysts ADPKD. This pericystic endothelium,...
ABSTRACT Introduction: Good medical practice dictates that comprehensive documentation of all surgical procedures is paramount in maintaining a high standard patient care. This study audited the quality operative note keeping for elective and trauma against standards set by British Orthopaedic Association (BOA) The Royal College Surgeons England (RCSE) guidelines. Patients methods: A retrospective assessment notes every undergoing total knee hip replacement (elective cases) was carried out...
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses threats to individuals with rare disease, in part because so little is known about the impact of COVID-19 infection and vaccination safety disease populations. Of particular concern, given overlap manifestations interferon dysregulation, are a group heritable autoinflammatory conditions called type I interferonopathies. most common these, Aicardi Goutières Syndrome (AGS), caused by altered nucleic acid metabolism...
Abstract Epidermal growth factor receptor (EGFR) is frequently mutated in non-small cell lung cancer (NSCLC) where it has been successfully targeted with tyrosine kinase inhibitors, but these are eventually overcome by drug resistance. Overcoming this therapeutic challenge hindered the poor understanding of how wild type (WT)-EGFR elicits ligand-independent signals essential for homeostasis, which then hijacked mutations. Ligand-independent signaling cannot be explained autoinhibited...