A5066513717- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Chronic Lymphocytic Leukemia Research
- Air Quality and Health Impacts
- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- Single-cell and spatial transcriptomics
- Organ Transplantation Techniques and Outcomes
- Viral Infectious Diseases and Gene Expression in Insects
- Cytomegalovirus and herpesvirus research
- Immunodeficiency and Autoimmune Disorders
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Cancer Immunotherapy and Biomarkers
- Noise Effects and Management
- Occupational and environmental lung diseases
- Cancer Research and Treatments
Columbia University
2016-2022
Columbia University Irving Medical Center
2015-2018
New York University
2009-2013
T cell responses to viruses are initiated and maintained in tissue sites; however, knowledge of human antiviral cells is largely derived from blood. Cytomegalovirus (CMV) persists most humans, requires immunity control, yet immune remain undefined. Here, we investigated CMV-specific cells, virus persistence CMV-associated homeostasis blood, lymphoid, mucosal secretory tissues 44 CMV seropositive 28 seronegative donors. were distinct distribution patterns, highest bone marrow (BM), or lymph...
Translating studies on T cell function and modulation from mouse models to humans requires extrapolating in vivo results responses lymphoid organs (spleen lymph nodes [LN]) human peripheral blood cells. However, our understanding of sites their relation remains sparse. In this study, we used a unique tissue resource study cells different anatomical compartments within individual donors identify subset memory CD8+ LN, which maintain distinct differentiation functional profile compared with...
Organ donors are sources of physiologically healthy organs and tissues for life-saving transplantation, have been recently used human immunology studies which typically confined to the sampling peripheral blood. Donors comprise a diverse population with different causes death clinical outcomes during hospitalization, effects such variations on immune parameters in blood not known. We present here coordinate analysis innate adaptive components blood, lymphoid (bone marrow, spleen, lymph...
Tumors are theoretically capable of eliciting an antitumor immune response, but often poorly immunogenic. Oncolytic viruses (OVs) have recently emerged as a promising strategy for the immunogenic delivery tumor-associated antigens (TAAs) to cancer patients. However, safe and effective OV/TAA therapies not yet been established. We previously demonstrated that vectors based on Sindbis virus (SV) can inhibit tumor growth activate innate system in mice. Here, we demonstrate SV carrying TAA...
B cell clones expand and contract during adaptive immune responses can persist or grow uncontrollably in lymphoproliferative disorders. One way to monitor track is perform large-scale sampling of bulk populations, amplifying sequencing antibody gene rearrangements by next generation (NGS). Here we describe a series computational approaches for estimating clone size NGS repertoire profiling data heavy chain rearrangements. We define three different measures size-- copy numbers, instances...
Oncolytic viruses (OVs) represent a relatively novel anti-cancer modality. Like other new cancer treatments, effective OV therapy will likely require combination with conventional treatments. In order to design combinatorial treatments that work well together, greater scrutiny of the mechanisms behind individual is needed. Sindbis virus (SV) based vectors have previously been shown target and kill tumors in xenograft, syngeneic, spontaneous mouse models. However, effect SV treatment on...
Tissue-resident memory T cells (TRM) in mice mediate optimal protective immunity to infection and vaccination, while humans, the existence properties of TRM remain unclear. Here, we use a unique human tissue resource determine whether comprise distinct subset diverse mucosal lymphoid tissues. We identify core transcriptional profile within CD69 CD4 CD8 lung spleen that is from CD69˗TEM tissues circulation, defines based on homology mouse TRM. Human sites exhibit increased expression adhesion...
Abstract Conventional dendritic cells (cDCs) are a rare population of that sense the environment and whose major role is in initiating controlling T cell immunity. The purpose this study was to generate map cDC subset distribution function over human life, which could serve as framework for understanding manipulating adaptive Current DC-based cancer therapies utilize DCs derived from blood monocytes, may differ tissue cDCs. We hypothesized analysis dynamics tissues uncover hitherto...