A5085300503- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Fungal Biology and Applications
- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolic Engineering and Bioproduction
- Marine Sponges and Natural Products
- Fermentation and Sensory Analysis
- Biochemical and Structural Characterization
- Probiotics and Fermented Foods
- Chemical Synthesis and Analysis
- Microbial Community Ecology and Physiology
- Meat and Animal Product Quality
- Actinomycetales infections and treatment
- Bacterial Genetics and Biotechnology
- Glycosylation and Glycoproteins Research
- Microbial Metabolism and Applications
- Plant Pathogens and Fungal Diseases
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Plant biochemistry and biosynthesis
- Biochemical and Molecular Research
- Antibiotic Resistance in Bacteria
- Polysaccharides Composition and Applications
Mylan (Switzerland)
2019
Biogen (Italy)
2015
Weatherford College
2007
Newron Pharmaceuticals (Italy)
2003-2006
Biosearch Technologies (United States)
1998-2001
ETH Zurich
1989
Genomics and metabolomics are widely used to explore specialized metabolite diversity. The Paired Omics Data Platform is a community initiative systematically document links between metabolome (meta)genome data, aiding identification of natural product biosynthetic origins structures.
<ns3:p>Background Innovative antibiotic discovery strategies are urgently needed to successfully combat infections caused by multi-drug-resistant bacteria. Methods We employed a direct screening approach identify compounds with antimicrobial and helper-drug activity against Gram-positive Gram-negative used this platform in two different strains of methicillin-resistant <ns3:italic>Staphylococcus aureus</ns3:italic> (MRSA) aminoglycoside-resistant <ns3:italic>Escherichia coli</ns3:italic>...
ABSTRACT A novel bacterial strain that was isolated from an Italian soil and designated SOSP1-21 T forms branched mycelia in solid liquid media has a filamentous morphology similar to of some genera belonging the Actinobacteria . Electron microscopy showed this organism grape-like appearance, resulting interlacing spores originating sporophoric hyphae. Ten strains are morphologically related were recovered soil. Phylogenetic analyses 16S rRNA gene segments confirmed relatedness these...
Actinomycetes play a relevant role in soil ecology and are also of important biotechnological interest as they produce several bioactive metabolites. Within the filamentous actinomycetes, it would be desirable to recognize characterize environmental samples containing unusual genera. To this end, we have developed selective primer sets for polymerase chain reaction (PCR) amplification 16S rDNA from Actinomycetales families Micromonosporaceae, Streptomycetaceae, Streptosporangiaceae...
The discovery of new antibiotics and other bioactive microbial metabolites continues to be an important objective in drug research. Since extensive screening has led the thousands molecules, approaches must taken order reduce probability rediscovering known compounds. authors have recently isolated slow-growing acidophiles belonging novel genera Catenulispora Actinospica within Actinomycetales. These strains, which likely belong a suborder, grow as filamentous mycelia, genome size around 8...
Among the growing family of ribosomally synthesized, post-translationally modified peptides, particularly intriguing are class III lanthipeptides containing triamino acid labionin. In course a screening program aimed at finding bacterial cell wall inhibitors, we discovered new lanthipeptide produced by an Actinoplanes sp. The molecule, designated NAI-112, consists 22 amino acids and contains N-terminal labionin C-terminal methyl-labionin. Unique among lanthipeptides, it carries 6-deoxyhexose...
Lantibiotics are ribosomally synthesized and post-translationally modified antimicrobial peptides containing thioether rings. In addition to these cross-links, the clinical candidate lantibiotic NAI-107 also possesses a C-terminal S-[(Z)-2-aminovinyl]-d-cysteine (AviCys) unique 5-chloro-l-tryptophan (ClTrp) moiety linked its potent bioactivity. Bioinformatic genetic analyses on biosynthetic gene cluster identified mibH mibD as genes encoding flavoenzymes responsible for formation of ClTrp...
Abstract The glycopeptide A40926, produced by the actinomycete Nonomuraea gerenzanensis , is precursor of dalbavancin, a second-generation antibiotic approved for clinical use in USA and Europe 2014 2015, respectively. final product biosynthetic pathway an O- acetylated form A40926 (acA40926). Glycopeptide biosynthesis N. dependent upon dbv gene cluster that encodes, addition to two essential positive regulators Dbv3 Dbv4, putative members two-component signal transduction system,...
The glycopeptide teicoplanin is used for the treatment of serious infections caused by Gram-positive pathogens. tcp gene cluster, devoted to biosynthesis in actinomycete Actinoplanes teichomyceticus, was isolated and characterized. From sequence analysis, cluster spans approximately 73 kb includes 39 ORFs participating biosynthesis, regulation, resistance export. Of these, 34 find a match at least one five clusters previously Putative roles could be assigned most genes. two...
Isolation of high molecular weight DNA fragments from soil, in excess 1 Mb, and sufficient quality for cloning into an Escherichia coli-streptomycete artificial chromosome vector is described. The combination indirect extraction cells, using a nycodenz technique, followed by lysis biomass immobilised agarose plugs, allowed Mb to be purified.
A novel, Gram-positive bacterial strain was isolated from forest soil. Among species with validly published names, the 16S rRNA gene sequence is related most closely (approx. 93 % similarity) to that of Sporichthya polymorpha DSM 43042 T . However, differently this species, it forms both vegetative and aerial mycelia. The hyphae are straight slightly flexuous, starting septate form chains more than 20 cylindrical spores a rugose surface. acidophilic, pH range for robust growth between 4.3...
We identified an Actinoallomurus strain producing NAI-107, a chlorinated lantibiotic effective against multidrug-resistant Gram-positive pathogens and previously reported from the distantly related genus Microbispora. Inclusion of KBr in production medium either or Microbispora producer readily afforded brominated variants which were designated as NAI-108. The other post-translational modifications naturally occurring this family (i.e., hydroxylation Pro-14 C-terminal decarboxylation)...
NAI-107, a lantibiotic produced by Microbispora sp. 107891, shows potent activity against multi-drug-resistant bacterial pathogens. It is as complex of related molecules, which unusual for ribosomally synthesized peptides. Here we describe the identification, characterization, and antibacterial congeners 107891 corallina NRRL 30420. These molecules differ presence two, one, or zero hydroxyl groups at Pro-14, chlorine Trp-4, and/or sulfoxide on thioether first lanthionine.