A5052755079- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Plant Disease Resistance and Genetics
- Mycobacterium research and diagnosis
- Legume Nitrogen Fixing Symbiosis
- Tuberculosis Research and Epidemiology
- Plant-Microbe Interactions and Immunity
- Gut microbiota and health
- Microbial infections and disease research
- Actinomycetales infections and treatment
- Bacterial Genetics and Biotechnology
- Plant Pathogens and Fungal Diseases
- Bacterial biofilms and quorum sensing
- Mycorrhizal Fungi and Plant Interactions
- Soil Carbon and Nitrogen Dynamics
- Antibiotic Use and Resistance
- Environmental DNA in Biodiversity Studies
- Animal Behavior and Welfare Studies
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Plant nutrient uptake and metabolism
University of Warwick
2016-2025
Public Health England
2021
Earlham Institute
2020
Instituto de Salud Carlos III
2015
Coventry University
2010-2013
Monash University
2012
Hospital Sant Joan de Déu Barcelona
2011
Microbiology Society
2007
Ghana Health Service
2006
University of Aberdeen
2005
A group-specific primer, F243 (positions 226 to 243, Escherichia coli numbering), was developed by comparison of sequences genes encoding 16S rRNA (16S rDNA) for the detection actinomycetes in environment with PCR and temperature or denaturing gradient gel electrophoresis (TGGE DGGE, respectively). The specificity forward primer combination different reverse ones tested genomic DNA from a variety bacterial strains. Most investigated could be separated TGGE both techniques giving similar...
Four hundred and seventy-five strains, which included 394 type cultures of Streptomyces representatives 14 other actinomycete genera, were studied. Overall similarities these strains for 139 unit characters determined by the S SM J coefficients clustering UPGMA algorithm. Test error overlap between phena defined within acceptable limits. Cluster-groups coefficient at 70·1% similarity (S) level 50% S-level. Clusters distinguished 77·5% 63% S-levels. Groupings obtained with two generally...
Magnetic susceptibility values for topsoils across England arc combined with data soil type, geochemistry and concentrations of magnetotactic bacteria in order to evaluate different theories explaining magnetism. Strongly magnetic soils unpolluted areas are found over weakly substrates dominated by ultrafine superparamagnetic grains. Magnetotactic present insufficient account strongly soils, crop burning is discounted as a major factor. A small number samples show high associated either...
There is growing understanding that the environment plays an important role both in transmission of antibiotic resistant pathogens and their evolution. Accordingly, researchers stakeholders world-wide seek to further explore mechanisms drivers involved, quantify risks identify suitable interventions. a clear value establishing research needs coordinating efforts within across nations order best tackle this global challenge. At international workshop late September 2017, scientists from 14...
Inflammatory bowel disease (IBD), is a debilitating group of chronic diseases including Crohn's Disease (CD) and ulcerative colitis (UC), which causes inflammation the gut affects millions people worldwide. At different taxonomic levels, structure microbiota significantly altered in IBD patients compared to that healthy individuals. However, it unclear how these IBD-affected bacterial groups are related other common bacteria gut, they connected across conditions at global scale.In this...
ObjectivesMultidrug-resistant Enterobacteriaceae pose a significant threat to public health. We aimed study the impact of sewage treatment effluent on antibiotic resistance reservoirs in river.
Polyethylene (PE) is one of the most recalcitrant carbon-based synthetic materials produced and, currently, ubiquitous plastic pollutant found in nature. Over time, combined abiotic and biotic processes are thought to eventually breakdown PE. Despite limited evidence biological PE degradation speculation that hydrocarbon-degrading bacteria within plastisphere an indication biodegradation, there no clear mechanistic understanding process. Here, using high-throughput proteomics, we...
A comprehensive multiphasic survey of the prevalence and transfer gentamicin resistance (Gmr) genes in different non-clinical environments has been performed. We were interested to find out whether Gmr described from clinical isolates can be detected environmental habitats hot spots identified. Furthermore, this study aimed evaluate impact selective pressure on abundance mobility genes. The included samples soils, rhizospheres, piggery manure, faeces cattle, laying broiler chickens,...
ABSTRACT Samples of effluent and soil were collected from a reed bed system used to remediate liquid waste wool finishing mill with high use quaternary ammonium compounds (QACs) compared samples agricultural soils. Resistance quotients aerobic gram-negative gram-positive bacteria ditallowdimethylammomium chloride (DTDMAC) cetyltrimethylammonium bromide (CTAB) established by plating onto nutrient agar containing 5 μg/ml or 50 DTDMAC CTAB. Approximately 500 isolates obtained screened for the...
ABSTRACT The prevalences of three sulfonamide resistance genes, sul1 , sul2 and sul3 sulfachloropyridazine (SCP) were determined in bacteria isolated from manured agricultural clay soils slurry samples the United Kingdom over a 2-year period. Slurry tylosin-fed pigs amended with SCP oxytetracycline was used for manuring. Isolates positive sul genes further screened presence class 1 2 integrons. Phenotypic to significantly higher isolates pig postapplication soil than those preapplication...
During the lifetime of a fermenter culture, soil bacterium S. coelicolor undergoes major metabolic switch from exponential growth to antibiotic production. We have studied gene expression patterns during this switch, using specifically designed Affymetrix genechip and high-resolution time-series fermenter-grown samples. Surprisingly, we find that actually consists multiple finely orchestrated switching events. Strongly coherent clusters genes show drastic changes in already many hours before...
The character state data obtained for clusters defined at the 77·5% S SM similarity level in phenetic numerical classification described by Williams et al. (1983) were used to construct a probabilistic identification matrix. 23 phena included major (19 Streptomyces, 2 Streptoverticillium and 'Nocardia' mediterranea) one minor cluster (Streptomyces fradiae). characters most diagnostic these selected using Sneath's CHARSEP DIACHAR programs. resulting matrix consisted of 41 × phena....
Our current understanding of Antarctic soils is derived from direct culture on selective media, biodiversity studies based clone library construction and analysis, quantitative PCR amplification specific gene sequences the application generic microarrays for microbial community analysis. Here, we investigated functional potential a soil at Mars Oasis Alexander Island in southern Maritime Antarctic, by applying 454 pyrosequencing technology to metagenomic constructed genomic DNA. The results...
The environment harbours a significant diversity of uncultured bacteria and potential source novel extant resistance genes which may recombine with clinically important disseminated into environmental reservoirs. There is evidence that pollution can select for due to the aggregation adaptive on mobile elements. aim this study was establish impact waste water treatment plant (WWTP) effluent disposal river by using culture independent methods downstream WWTP in comparison upstream. Metagenomic...
The clinical failure of antimicrobial drugs that were previously effective in controlling infectious disease is a tragedy increasing magnitude gravely affects human health. This resistance by pathogens often the endpoint an evolutionary process began billions years ago non-disease-causing microorganisms. environmental resistome, its mobilization, and conditions facilitate entry into are at heart current public health crisis antibiotic resistance. Understanding origins, evolution, mechanisms...