A5087088506- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Legume Nitrogen Fixing Symbiosis
- Microbial Natural Products and Biosynthesis
- Antimicrobial Peptides and Activities
- Invertebrate Immune Response Mechanisms
- Phytoplasmas and Hemiptera pathogens
- Biochemical and Structural Characterization
- Banana Cultivation and Research
- Sugarcane Cultivation and Processing
- Insect and Pesticide Research
- Plant Pathogens and Fungal Diseases
- Enzyme Production and Characterization
- Plant responses to water stress
- Antibiotic Resistance in Bacteria
- Plant pathogens and resistance mechanisms
- Carbohydrate Chemistry and Synthesis
- Plant tissue culture and regeneration
- Insect and Arachnid Ecology and Behavior
- Malaria Research and Control
- Mycotoxins in Agriculture and Food
- Fungal Biology and Applications
- Pineapple and bromelain studies
- Infections and bacterial resistance
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2011-2023
Centre Occitanie-Montpellier
2019-2023
Plant Health Institute de Montpellier
2019-2023
Agropolis International
2019-2023
Institut Agro Montpellier
2007-2022
Université de Montpellier
2017-2022
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022
L'Institut Agro
2022
Institut de Recherche pour le Développement
2022
Biologie et Génétique des Interactions Plante-Parasite
2007-2017
Insect defensins are cationic, cysteine-rich peptides (approximately 4 kDa) that appear after bacterial challenge or injury in the hemolymph of insects belonging to a large variety orders. These possess anti-Gram-positive activity and participate potent antibacterial defense reactions insects. Using recombinant insect defensin strain Micrococcus luteus as test organism, we have investigated mode action this peptide. We show disrupts permeability barrier cytoplasmic membrane M. luteus,...
Injection of heat-killed bacteria into larvae the large tenebrionid beetle Zophobas atratus (Insecta, Endopterygota, Coleoptera) results in appearance hemolymph a potent antibacterial activity as evidenced by plate growth inhibition assay. We have isolated three peptides (A-C) from this immune which probably account for most activity. Their primary structures were established combination peptide sequencing and molecular mass determination spectrometry. Peptide A, is bactericidal against...
Insects belonging to the recent orders of endopterygote clade (Lepidoptera, Diptera, Hymenoptera and Coleoptera) respond bacterial challenge by rapid transient synthesis a battery potent antibacterial peptides which are secreted into their haemolymph. Here we present first report on inducible molecules in sap-sucking bug Pyrrhocoris apterus, representative species Hemiptera, predated Endoptergotes at least 50 million years evolution. We have isolated characterized from immune blood this...
The Xanthomonadaceae family contains two xylem-limited plant pathogenic bacterial species, Xanthomonas albilineans and Xylella fastidiosa. X. fastidiosa was the first completely sequenced pathogen. It is insect-vectored, has a reduced genome does not possess hrp genes which encode Type III secretion system found in most bacteria. excluded from group based on phylogenetic analyses with rRNA sequences.The complete of annotated. albilineans, known to be also genes. Phylogenetic analysis using...
The injection of low doses bacteria into the aquatic larvae dragonflies (Aeschna cyanea, Odonata, Paleoptera) induces appearance in their hemolymph a potent antibacterial activity. We have isolated 38-residue peptide from this which is strongly active against Gram-positive and also shows activity one Gram-negative was tested. novel member insect defensin family inducible peptides, had so far only been reported higher orders believed to evolved 100 million years after Paleoptera. Aeschna more...
Xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. X. exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species Xanthomonas. For example, this produces potent DNA gyrase inhibitor called albicidin that is largely responsible for inducing symptoms; its habitat limited xylem; the large variability. A first manuscript on complete genome sequence highly strain GPE PC73 focused exclusively genomic features shared with Xylella...
Abstract Background Xanthomonads are plant-associated bacteria responsible for diseases on economically important crops. Xanthomonas fuscans subsp . ( Xff ) is one of the causal agents common bacterial blight bean. In this study, complete genome sequence strain 4834-R was determined and compared to other sequences. Results Comparative genomics analyses revealed core characteristics shared between xanthomonads including chemotaxis elements, two-component systems, TonB-dependent transporters,...
Abstract The peptide antibiotic albicidin, which is synthesized by the plant pathogenic bacterium Xanthomonas albilineans , displays remarkable antibacterial activity against various Gram‐positive and Gram‐negative microorganisms. low amounts of albicidin obtainable from producing organism or through heterologous expression are limiting factors in providing sufficient material for bioactivity profiling structure–activity studies. Therefore, we developed a convergent total synthesis route...
The para-aminobenzoic acid-containing peptide albicidin is a pathogenicity factor synthesized by Xanthomonas albilineans in infections of sugar cane. Albicidin nanomolar inhibitor the bacterial DNA gyrase with strong activity against various Gram-negative bacteria. bacterium Pantoea dispersa expresses hydrolase AlbD, conferring natural resistance albicidin. We show that AlbD novel type endopeptidase catalyzes cleavage at backbone amide bond, thus abolishing its antimicrobial activity....
Natural products represent an important source of potential novel antimicrobial drug leads. Low production by microorganisms in cell culture often delays the structural elucidation or even prevents a timely discovery. Starting from anti-Gram-negative antibacterial compound albicidin produced Xanthomonas albilineans, we describe bioactivity-guided approach combined with non-targeted tandem mass spectrometry and spectral (molecular) networking for discovery compounds. We report eight new...
Xanthomonas albilineans is the bacterium responsible for leaf scald, a lethal disease of sugarcane. Within genus, X. exhibits distinctive genomic characteristics including presence significant genome erosion, non-ribosomal peptide synthesis (NRPS) locus involved in albicidin biosynthesis, and type 3 secretion system (T3SS) Salmonella pathogenicity island-1 (SPI-1) family. We sequenced two albilineans-like strains isolated from unusual environments, i.e., dew droplets on sugarcane leaves wild...
Various bacteria can use non-ribosomal peptide synthesis (NRPS) to produce peptides or other small molecules. Conserved features within the NRPS machinery allow type, and sometimes even structure, of synthesized polypeptide be predicted. Thus, bacterial genome mining via in silico analyses genes offers an attractive opportunity uncover new bioactive non-ribosomally peptides. Xanthomonas is a large genus Gram-negative that cause disease hundreds plant species. To date, only known molecule by...
ABSTRACT Pathogenicity of 75 strains Xanthomonas albilineans from Guadeloupe was assessed by inoculation sugarcane cv. B69566, which is susceptible to leaf scald, and 19 the were selected as representative variation in pathogenicity observed based on stalk colonization. In vitro production albicidin varied among these strains, but restriction fragment length polymorphism pattern their biosynthesis genes identical. Similarly, no genomic found pulsed-field gel electrophoresis. Some amplified...
Xanthomonas albilineans is the causal agent of sugarcane leaf scald. Interestingly, this bacterium, which not known to be insect or animal associated, possesses a type III secretion system (T3SS) belonging injectisome family Salmonella pathogenicity island 1 (SPI-1). The T3SS SPI-1 X. shares only low similarity with other available sequences. Screening collection 128 plant-pathogenic bacteria revealed that present in two species Xanthomonas: and axonopodis pv. phaseoli. Inoculation knockout...
Albicidin is a potent antibiotic and phytotoxin produced by Xanthomonas albilineans which targets the plant bacterial DNA gyrase. We now report on new albicidin derivative carbamoylated at N-terminal coumaric acid action of ATP-dependent O-carbamoyltransferase Alb15, present in (alb) gene cluster. Carbamoyl-albicidin was characterized tandem mass spectrometry from cultures overproducer strain function confirmed inactivation alb15 X. albilineans. Expression Escherichia coli vitro...
Summary The Vfm quorum sensing (QS) system is preponderant for the virulence of different species bacterial genus Dickeya . vfm gene cluster encodes 26 genes involved in production, or transduction QS signal. To date, signal has escaped detection by analytical chemistry methods. However, we report here a strain‐specific polymorphism biosynthesis vfmO and vfmP , which predicted to be related production analogues Consequently, communication could impossible between strains possessing variants...
The phytotoxin and polyketide antibiotic albicidin produced by Xanthomonas albilineans is a highly potent DNA gyrase inhibitor. Low yields of production have slowed studies its chemical structure. Heterologous expression biosynthetic genes in X. axonopodis pv. vesicatoria resulted sixfold increase production, offering promising strategies for engineering overproduction.
Abstract Das Antibiotikum Albicidin wird von dem pflanzenpathogenen Bakterium Xanthomonas albilineans synthetisiert. Es zeigt eine bemerkenswerte Aktivität gegen Gram‐positive und Gram‐negative Mikroorganismen. Der Wildtypstamm wie auch der heterologe Expressionsstamm produziert bislang in äußerst geringen Mengen, was die Untersuchung des biologischen Profils Ausarbeitung Struktur‐Aktivitäts‐Beziehungen erschwert. Aus diesem Grund entwickelten wir konvergente Syntheseroute für Albicidin. Die...