A5019020069- Respiratory viral infections research
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Tracheal and airway disorders
- Pneumonia and Respiratory Infections
- Neonatal Respiratory Health Research
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- Congenital Diaphragmatic Hernia Studies
- Cytomegalovirus and herpesvirus research
- Pediatric health and respiratory diseases
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Travel-related health issues
- SARS-CoV-2 detection and testing
- Parvovirus B19 Infection Studies
- Mosquito-borne diseases and control
- Asthma and respiratory diseases
- COVID-19 diagnosis using AI
- COVID-19 epidemiological studies
- Viral Infections and Outbreaks Research
Instituto de Salud Carlos III
2016-2025
Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública
2012-2025
Centro Nacional de Microbiologia
2013-2024
National Institute of Public Health
2024
Stanford University
2023
Hospital Universitario La Paz
2021-2023
Centro de Investigación Biomédica en Red
2023
Hospital La Paz Institute for Health Research
2023
Spanish Clinical Research Network
2018-2022
National Influenza Center
2012-2021
In the WHO European Region, COVID-19 surveillance was implemented 27 January 2020. We detail first cases. As at 21 February, nine countries reported 47 Among 38 cases studied, were linked to two clusters in Germany and France, 14 infected China. Median case age 42 years; 25 male. Late detection of clusters’ index delayed isolation further local 5 March, there 4,250
There is a need for rapid, sensitive, and accurate diagnosis of lower respiratory tract infections in children, elderly, immunocompromised patients, who are susceptible to serious complications. The multiplex RT-nested PCR assay has been used widely simultaneous detection non-related viruses involved infectious diseases because its high specificity sensitivity. A new RT-PCR described this report. This approach includes nested primer sets targeted conserve regions human parainfluenza virus...
The clinical presentation of infections caused by the heterogeneous group respiratory viruses can be very similar. Thus, implementation virological assays that rapidly identify most important involved is great interest. A new multiplex reverse transcription nested-polymerase chain reaction (RT-PCR) assay able to detect and type different simultaneously described. Primer sets were targeted conserved regions nucleoprotein genes influenza viruses, fusion protein syncytial (RSV), hexon...
We have designed a study with the objective of describing clinical impact other viruses different from respiratory syncytial virus (RSV) in hospitalized infants bronchiolitis.A 3 year prospective was conducted on admitted to Paediatrics Department Severo Ochoa Hospital (Spain). studied frequency 16 viruses. Clinical characteristics RSV-only infections were compared single agent viral infections.Positive results confirmed 275 (86.5%) 318 children studied. A detected 196 patients and 79 dual...
ABSTRACT The emergence of natural isolates human respiratory syncytial virus group B (HRSV-B) with a 60-nucleotide (nt) duplication in the G protein gene Buenos Aires, Argentina, 1999 (A. Trento et al., J. Gen. Virol. 84:3115-3120, 2003) and their dissemination worldwide allowed us to use duplicated segment as tag examine detail evolution HRSV during propagation its host. Viruses were all clustered new genotype, named BA 80:975-984, 2006). To obtain information about prevalence these viruses...
Abstract The aim of the study was to determine incidence viruses causing aseptic meningitis, meningoencephalitis, and encephalitis in Spain. This a prospective study, collaboration with 17 Spanish hospitals, including 581 cases (CSF from all sera 280): meningitis (340), meningoencephalitis (91), (76), febrile syndrome (7), other neurological disorders (32), 35 without clinical information. CSF were assayed by PCR for enterovirus (EV), herpesvirus (herpes simplex [HSV], varicella‐zoster...
Influenza A virus (IAV) infection can be severe or even lethal in toddlers, the elderly and patients with certain medical conditions. Infection of apparently healthy individuals nonetheless accounts for many disease cases deaths, suggesting that viruses increased pathogenicity co-circulate pandemic epidemic viruses. Looking potential virulence factors, we have identified a polymerase PA D529N mutation detected fatal IAV case, whose introduction into two different recombinant backbones, led...
Community-acquired pneumonia (CAP) remains a significant cause for childhood morbidity worldwide. We designed study with the objective of describing frequency respiratory viruses, especially rhinovirus (RV), human metapneumovirus (HMPV) and bocavirus (HBoV) in hospitalized children CAP.A 6-year prospective was conducted <14 years old admitted to Pediatrics Department Severo Ochoa Hospital (Spain) CAP. studied 16 viruses nasopharyngeal aspirates. Clinical characteristics syncytial virus...
BACKGROUND. Passive immunotherapy with convalescent plasma (CP) is a potential treatment for COVID-19. Evidence from controlled clinical trials inconclusive.
Multiple SARS-CoV-2 introductions have been detected in Spain, and at least four resulted the emergence of locally transmitted clusters that originated not later than mid-February, with further dissemination to many other countries around world, a few weeks before explosion COVID-19 cases Spain during first week March. The majority earliest variants branched clade 19B (D614 viruses), which was most prevalent March, pointing founder effect. However, from mid-March June 2020, G614-bearing...
Abstract The main objective was to evaluate the viability of SARS-CoV-2 viral particles excreted in stools. In addition, we aimed identify clinical factors associated with detection RNA feces, and determine if its presence is an unfavorable outcome, defined as intensive care unit (ICU) admission and/or death. A prospective multicenter cohort study COVID-19 adult patients, confirmed infection by RT-PCR assay nasopharyngeal (NP) swabs admitted four hospitals Spain, from March 2020 February...
In autumn 2022, the Spanish Influenza National Reference Laboratory (NRL) confirmed detection of influenza A(H5N1) in samples from two asymptomatic workers linked to an outbreak a poultry farm Spain. Nasopharyngeal swabs were taken according national screening protocol for exposed workers. Absence symptoms, low viral load and negative serology both suggested environmental contamination. These findings motivated update early strategy specifying timing sampling conditions persons.
Abstract Naturally emerging and deliberately released pathogens demand new detection strategies to allow early recognition containment. We describe a diagnostic system for rapid, sensitive, multiplex discrimination of microbial gene sequences report its application detecting 22 respiratory in clinical samples.
The role of viruses in community-acquired pneumonia may have been previously underestimated. We aimed to study the incidence and clinical characteristics (CAP) due respiratory adults adding PCR routine conventional laboratory tests.Consecutive adult patients diagnosed CAP from January 2003 March 2004 were included. Conventional tests including cultures blood, sputum, urine antigen detection Streptococcus pneumoniae Legionella pneumophila, paired serologies routinely performed. Nasopharyngeal...
Abstract Three nested RT‐PCR assays were developed to permit sensitive typing of enteroviruses directly from clinical samples. These amplified short fragments different genomic regions codifying for three proteins: VP2, VP1, and RNA polymerase. Given that have a high rate degeneration within target codons among serotypes, the primers used consisted mixed base deoxyinosine residues. techniques detected at 0.03–0.003 TCID 50 prototype Poliovirus 1 Echovirus 30. They characterize enteroviral in...
Acute aseptic meningitis (AAM) is considered as an uncommon manifestation of varicella-zoster virus (VZV) recrudescence and usually regarded a complication the cutaneous infection in patients with impaired cellular immunity. Indirect evidence suggests, however, that VZV-associated AAM may also respond to direct spread leptomeninges from cells supporting latency. The polymerase chain reaction (PCR) was used amplify VZV-specific DNA sequences serial cerebrospinal fluid (CSF) samples 21 AAM,...
Abstract Background Human metapneumovirus (hMPV) infection plays an important role in the pediatric respiratory infections. However, little is known about relationship between hMPV‐bronchiolitis and development of subsequent wheezing. The aim this study was to evaluate outcome at third fifth year after admission for compare it with children admitted syncytial virus (RSV)‐bronchiolitis without lower disease first 2 years life. Methods We studied 55 (23 hMPV 32 RSV acute bronchiolitis) aged...
Abstract Human metapneumovirus (hMPV), a condition recently described in the Netherlands, causes lower respiratory infections, particularly young children and among elderly. The objective of this study was to describe characteristics hMPV infections hospitalized infants <2 years age compare them those caused by syncytial virus (RSV). A prospective conducted on clinical admitted hospital for infection through 5 years. Simultaneous detection influenza A, B, C viruses, RSV, adenoviruses...