Nicholas Di Paola
A5027383447- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Plant Virus Research Studies
- Vector-Borne Animal Diseases
- Respiratory viral infections research
- Congenital Diaphragmatic Hernia Studies
- Fire effects on ecosystems
- Virology and Viral Diseases
- Viral gastroenteritis research and epidemiology
- Poxvirus research and outbreaks
- Bacillus and Francisella bacterial research
- RNA modifications and cancer
- Aquaculture disease management and microbiota
- Herpesvirus Infections and Treatments
- Parvovirus B19 Infection Studies
- Malaria Research and Control
- COVID-19 epidemiological studies
- CRISPR and Genetic Engineering
- COVID-19 diagnosis using AI
- Animal Disease Management and Epidemiology
- Long-Term Effects of COVID-19
- Vector-borne infectious diseases
- Plant and Fungal Interactions Research
- Animal Virus Infections Studies
United States Army Medical Research Institute of Infectious Diseases
2019-2024
University of Nebraska Medical Center
2020-2022
Institut de Recherche en Sciences de la Santé
2022
Centre Hospitalier Universitaire Yalgado Ouédraogo
2022
Noguchi Memorial Institute for Medical Research
2022
Institut Pasteur de Dakar
2020
University of Kinshasa
2020
Fred Hutch Cancer Center
2020
Institut de Recherche pour le Développement
2020
Université de Montpellier
2020
The 2023 monkeypox (mpox) epidemic was caused by a subclade IIb descendant of virus (MPXV) lineage traced back to Nigeria in 1971. Person-to-person transmission appears higher than for clade I or IIa MPXV, possibly genomic changes MPXV. Key could occur the genome's low-complexity regions (LCRs), which are challenging sequence and often dismissed as uninformative. Here, using combination highly sensitive techniques, we determine high-quality MPXV genome representative current with LCRs...
The West Nile virus (WNV), isolated in 1937, is an arbovirus (arthropod-borne virus) that infects thousands of people each year. Despite its burden on global health, little known about the virus' biological and evolutionary dynamics. As several lineages are endemic Africa, we obtained complete polyprotein sequence from three isolates early 1990s, representing a different lineage. We then investigated differences growth behavior pathogenicity for four distinct African arthropod (Ap61) primate...
From November 2018 through February 2019, person-to-person transmission of Andes virus (ANDV) hantavirus pulmonary syndrome occurred in Chubut Province, Argentina, and resulted 34 confirmed infections 11 deaths. Understanding the genomic, epidemiologic, clinical characteristics ANDV is crucial to designing effective interventions.Clinical epidemiologic information was obtained by means patient report from public health centers. Serologic testing, contact-tracing, next-generation sequencing...
Filoviridae is a family of negative-sense RNA viruses with genomes about 13.1–20.9 kb that infect fish, mammals and reptiles. The filovirid genome linear, non-segmented five canonical open reading frames (ORFs) encode nucleoprotein (NP), polymerase cofactor (VP35), glycoprotein (GP 1,2 ), transcriptional activator (VP30) large protein (L) containing an RNA-directed (RdRP) domain. All additional proteins vary among genera. Several filovirids (e.g., Ebola virus, Marburg virus) are pathogenic...
Usutu virus (USUV) is a Culex-associated mosquito-borne flavivirus of the Flaviviridae family. Since its discovery in 1959, has been isolated from birds, arthropods and humans Europe Africa. An increasing number infections with neurological presentations have reported. Recently, detected bats horses, which deviates currently proposed enzootic cycle USUV involving several different avian mosquito species. Despite this viral detections mammalian hosts, existence non-avian reservoir remains...
Crimean–Congo haemorrhagic fever virus (CCHFV) is the medically most important member of rapidly expanding bunyaviral family Nairoviridae . Traditionally, CCHFV isolates have been assigned to six distinct genotypes. Here, International Committee on Taxonomy Viruses (ICTV) Study Group outlines reasons for recent decision re-classify genogroup VI (aka Europe-2 or AP-92-like) as a virus, Aigai (AIGV).
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causing an exponentially increasing number of disease 19 (COVID-19) cases globally. Prioritization medical countermeasures for evaluation in randomized clinical trials critically hindered by the lack COVID-19 animal models that enable accurate, quantifiable, and reproducible measurement pulmonary free from observer bias. We first used serial computed tomography (CT) to demonstrate bilateral intrabronchial instillation SARS-CoV-2...
European perch (Perca fluviatilis) are increasingly farmed as a human food source. Viral infections of remain largely unexplored, thereby putting farm populations at incalculable risk for devastating fish epizootics and presenting potential hazard to consumers. To address these concerns, we applied metatranscriptomics identify disease-associated viruses in Switzerland. Unexpectedly, clinically diseased detected novel freshwater filoviruses, hantavirus, previously unknown rhabdovirus....
Abstract Knowledge of contemporary genetic composition dengue virus (DENV) in Africa is lacking. By using next-generation sequencing samples from the 2017 DENV outbreak Burkina Faso, we isolated 29 genomes (5 serotype 1, 16 2 [DENV-2], and 8 3). Phylogenetic analysis demonstrated endemic nature DENV-2 Faso. We noted discordant diagnostic results, probably related to divergence between these Trioplex PCR. Forward reverse1 primers had a single mismatch when mapped genomes, explaining...
Jingchuvirales is an order of negative-sense RNA viruses with genomes 9.1–15.3 kb that have been associated arachnids, barnacles, crustaceans, insects, fish and reptiles in Africa, Asia, Australia, Europe, North America South America. The jingchuviral genome has two to four open reading frames (ORFs) encode a glycoprotein (GP), nucleoprotein (NP), large (L) protein containing RNA-directed polymerase (RdRP) domain, and/or proteins unknown function. Viruses the are only known from their...
We followed the presence of Zika virus (ZIKV) in four healthy adults (two men and two women), for periods ranging from 78 to 298 days post symptom onset. The patients were evaluated regarding different body fluids (blood, saliva, urine semen), development immune responses (including antibodies, cytokines chemokines), genetic variation within samples collected semen during infection course. analysis was focused primarily on male who shed up 158 after initial symptoms. ZIKV particles detected...
Abstract We detected Marburg virus RNA in rectal swab samples from Egyptian rousette bats South Africa 2017. This finding signifies that fecal contamination of natural bat habitats is a potential source infection for humans. Identified genetic sequences are closely related to Ravn virus, implying wider distribution Africa.