A5044535165- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Vector-Borne Animal Diseases
- Malaria Research and Control
- Vibrio bacteria research studies
- Fire effects on ecosystems
- COVID-19 epidemiological studies
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Zoonotic diseases and public health
- Insect symbiosis and bacterial influences
- Virology and Viral Diseases
- Retinal Diseases and Treatments
- Genomics and Phylogenetic Studies
- Genetic and phenotypic traits in livestock
- Vector-borne infectious diseases
- Vaccine Coverage and Hesitancy
- Viral gastroenteritis research and epidemiology
- Retinal and Optic Conditions
- Animal Disease Management and Epidemiology
Institut Pasteur de Dakar
2017-2025
Institut Pasteur
2010-2015
The West Nile virus (WNV), isolated in 1937, is an arbovirus (arthropod-borne virus) that infects thousands of people each year. Despite its burden on global health, little known about the virus' biological and evolutionary dynamics. As several lineages are endemic Africa, we obtained complete polyprotein sequence from three isolates early 1990s, representing a different lineage. We then investigated differences growth behavior pathogenicity for four distinct African arthropod (Ap61) primate...
BackgroundYellow fever vaccine is highly effective with a single dose, but supply limited. The minimum dose requirements for seroconversion remain unknown.MethodsIn this double-blind, randomized, noninferiority trial in Uganda and Kenya, we assigned adults no history of yellow vaccination or infection to receive the Institut Pasteur de Dakar 17D-204 at standard (13,803 IU) fractional 1000 IU, 500 250 IU. primary outcome was 28 days after each as compared evaluated analysis. Seroconversion...
BackgroundStocks of yellow fever vaccine are insufficient to cover exceptional demands for outbreak response. Fractional dosing has shown efficacy, but evidence is limited the 17DD substrain vaccine. We assessed immunogenicity and safety one-fifth fractional dose compared with standard four WHO-prequalified vaccines produced from three substrains.MethodsWe did this randomised, double-blind, non-inferiority trial at research centres in Mbarara, Uganda, Kilifi, Kenya. Eligible participants...
Yellow fever (YF) virus is a mosquito-borne belonging to the Flaviviridae family that circulates in tropical and subtropical areas of Africa South America. Despite availability an effective vaccine, YF remains threat travelers, residents endemic areas, unvaccinated populations. vaccination natural infection both induce production neutralizing antibodies. Serological diagnostic methods detecting virus-specific antibodies demonstrate high levels cross-reactivities with other flaviviruses. To...
Bataï virus (BATV), belonging to the Orthobunyavirus genus, is an emerging mosquito-borne with documented cases in Asia, Europe, and Africa. It causes various symptoms humans ruminants. Another related Ilesha (ILEV), which a range of diseases mainly found African countries. This study aimed genetically identify characterize BATV strain previously misclassified as ILEV Senegal. The was reactivated subjected whole genome sequencing using Illumina-based approach. Genetic analyses phylogeny were...
Crimean-Congo haemorrhagic fever (CCHF) is a severe zoonotic arboviral disease that occurs widely in Eastern and Western Europe, Asia Africa. The becoming of growing public health importance Senegal. However, analysis tick infestation, CCHF virus (CCHFV) circulation extent risk factors during ongoing outbreak are scarce. A thorough investigation was carried out Podor (Northern Senegal) August 2022. Ticks blood samples were collected from animals (cattle, goats sheep) randomly selected...
A nationwide cross-sectional epidemiological survey was conducted to capture the true extent of coronavirus disease 2019 (COVID-19) exposure in Senegal. Multi-stage random cluster sampling households performed between October and November 2020, at end first wave COVID-19 transmission. Anti-severe acute respiratory syndrome 2 (SARS-CoV-2) antibodies were screened using three distinct ELISA assays. Adjusted prevalence rates for design calculated each test separately, thereafter combined. Crude...
Orthobunyaviruses are a diverse group of segmented RNA viruses with significant but underexplored public and veterinary health implications. This study provides genomic, phylogenetic, ecological analysis neglected using next-generation sequencing computational predictions. We identified unique phylogenetic relationships, Tanga virus forming distinct lineage linked to zoonotic, human-associated, or non-vertebrate across segments. GC content revealed segment-specific patterns: higher in the S...
Rift Valley Fever and Crimean-Congo Hemorrhagic are two infections classified among the emerging diseases to be monitored with highest priority. Studies undertaken in human animals have shown endemicity of these arboviruses several African countries. However, most investigations were carried out on domestic cattle studies conducted populations either outdated or limited a small number well-known endemic areas. It is then critical better evaluate burden viruses Senegal at national scale.This...
Evidence indicates that fractional doses of yellow fever vaccine are safe and sufficiently immunogenic for use during outbreaks. However, there no data on the generalisability this observation to populations living with HIV. Therefore, we aimed evaluate immunogenicity standard in HIV-positive adults.
SUMMARY In 2005, a serological study was carried out on horses in five ecologically contrasted zones of the Senegal River basin (Senegal) to assess West Nile virus (WNV) transmission and investigate underlying environmental risk factors. each zone, were randomly selected blood samples taken. A land-cover map areas built using two satellite ETM+ images. Blood screened by ELISA for anti-WNV IgM IgG positive confirmed seroneutralization. Environmental data analysed principal components...
Zika virus (ZIKV) diagnostics are crucial for proper antenatal and postnatal care also surveillance serosurvey studies. Since the viremia during ZIKV infection is fleeting, serological testing highly valuable to inform diagnosis. However, current serology tests using whole antigens frequently suffer from cross reactivity issues, delays, technical complexity, especially in low middle income countries (LMICs) endemic countries. Here, we describe an indirect ELISA detect specific IgG antibodies...
Rift Valley fever (RVF) is a re-emerging vector-borne zoonosis with high public health and veterinary impact. In West Africa, many lineages were previously detected, but since 2020, lineage H from South Africa has been the main cause of outbreaks. this study, clinical samples collected through national surveillance screened for RVF virus (RVFV) acute infection by RT-PCR IgM ELISA tests. Sequencing, genome mapping in vitro phenotypic characterization mammal cells performed on positive...
West Nile virus (WNV), belonging to the Flaviviridae family, causes a mosquito-borne disease and shows great genetic diversity, with at least eight different lineages. The Koutango lineage of WNV (WN-KOUTV), mostly associated ticks rodents in wild, is exclusively present Africa evidence infection humans high virulence mice. In 2016, context Rift Valley fever (RVF) outbreak Niger, mosquitoes, biting midges sandflies were collected for arbovirus isolation using cell culture, immunofluorescence...
West Nile Virus (WNV) is the most widely distributed flavivirus worldwide. It a mosquito-borne virus, and birds constitute its natural reservoir. Humans equines are considered accidental hosts. Human WNV infections usually asymptomatic or express as mild febrile syndrome; however, in around 1% of cases they responsible for more serious neurological diseases with potentially lethal outcome. In Mediterranean basin virus circulation regarded endemic. Outbreaks meningoencephalitis regularly...
Wesselsbron is a neglected, mosquito-borne zoonotic disease endemic to Africa. The virus mainly transmitted by the mosquitoes of Aedes genus and primarily affects domestic livestock species with teratogenic effects but can jump humans. Although no major outbreak or fatal case in humans has been reported as yet worldwide, total 31 acute human cases infection have previously described since its first isolation 1955. However, most these were from Sub-Saharan Africa where resources are limited...
Background: Senegal reported the first COVID-19 case on March 2, 2020. A nationwide cross-sectional epidemiological survey was conducted to capture true extent of exposure. Methods: Multi-stage random cluster sampling households carried out between October 24 and November 26, 2020, at end wave transmission. Anti-SARS-CoV-2 antibodies (IgG and/or IgM) were screened using three distinct ELISA assays. Adjusted prevalence for design calculated each test separately, thereafter combined. Crude,...
Abstract The Chikungunya virus, a global arbovirus, is currently causing major outbreak in the Western African region, with highest cases reported Senegal and Burkina Faso. Recent molecular evolution analyses reveal that strain responsible for epidemic belongs to West genotype, new mutations potentially impacting viral replication, antigenicity, host adaptation. Real-time genomic monitoring needed track virus’s spread regions. A scalable genotype amplicon-based Whole Genome Sequencing...