A5054895441- Venomous Animal Envenomation and Studies
- Healthcare and Venom Research
- Rabies epidemiology and control
- Ion channel regulation and function
- Marine Invertebrate Physiology and Ecology
- Entomological Studies and Ecology
- Trypanosoma species research and implications
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Lipid Membrane Structure and Behavior
- Research on Leishmaniasis Studies
- Complement system in diseases
- Toxin Mechanisms and Immunotoxins
- Insect and Pesticide Research
- Allergic Rhinitis and Sensitization
- Erythrocyte Function and Pathophysiology
- Cellular transport and secretion
- Vector-borne infectious diseases
- Bioactive Natural Diterpenoids Research
- Blood Coagulation and Thrombosis Mechanisms
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Beetle Biology and Toxicology Studies
- Neuropeptides and Animal Physiology
- Signaling Pathways in Disease
Instituto Butantan
2016-2025
Fundação de Amparo à Pesquisa do Estado de São Paulo
2024
Universidade Cidade de São Paulo
2005
University of Wales
2000
Universidade de São Paulo
1989-1998
Envenomings by snakebites and scorpion stings impose a high burden worldwide result in considerable social economic impact [1]. It is estimated that snakebite rates are as over 1.8 million cases per year, with associated deaths reaching more than 90,000 annually [2]. However, neglected condition no WHO programmes for control prevention. The countries most affected located the intertropical zone areas of field use agriculture where main populations adult men working agricultural activities...
Bites by Loxosceles spiders can produce severe clinical symptoms, including dermonecrosis, thrombosis, vascular leakage, hemolysis, and persistent inflammation. The causative factor is a sphingomyelinase D (SMaseD) that cleaves sphingomyelin into choline ceramide 1-phosphate. A similar enzyme, showing comparable bioactivity, secreted certain pathogenic corynebacteria acts as potent virulence factor. However, the molecular basis for SMaseD toxicity not well understood, which hampers effective...
Adding to antibodies: Ordered mesoporous silicas such as SBA-15 may be used adjuvants carry, protect, and deliver entrapped antigens, thereby assuring efficient stimulation of antigen-presenting cells improving antibody responsiveness. Thus, related silica mesostructures seem promising nanosystems for vaccine delivery. Venom proteins from the Micrurus ibiboboca snake (see picture) were utilized in study.
Abstract Background The bite of spiders belonging to the genus Loxosceles can induce a variety clinical symptoms, including dermonecrosis, thrombosis, vascular leakage, haemolysis, and persistent inflammation. In order examine transcripts expressed in venom gland laeta spider unveil potential its products on cellular structure functional aspects, we generated 3,008 sequence tags (ESTs) from cDNA library. Results All ESTs were clustered into 1,357 clusters, which 16.4% total belong recognized...
In 2006, the first report of a nanostructured material as adjuvant was described establishing effectiveness ordered mesoporous SBA-15 silica an immune adjuvant. The present study evaluated capacity to modulate responsiveness High and Low responder mice immunized with BSA encapsulated/adsorbed in by intramuscular or oral route effect compared induced aluminum hydroxide salts emulsified Incomplete Freund These results demonstrate ability non-toxic nanoparticles increase immunogenicity repair...
Micrurus snake bites can cause death by muscle paralysis and respiratory arrest, few hours after envenomation. The specific treatment for coral envenomation is the intravenous application of heterologous antivenom and, in Brazil, it produced horse immunization with a mixture M. corallinus frontalis venoms, snakes that inhabit South Southeastern regions country. However, this might be inefficient, considering existence intra- inter-specific variations composition venoms. Therefore, aim...
Sphingomyelinases D (SMases D) from Loxosceles spider venom are the principal toxins responsible for manifestation of dermonecrosis, intravascular hemolysis, and acute renal failure, which can result in death. These enzymes catalyze hydrolysis sphingomyelin, resulting formation ceramide 1-phosphate choline or lysophosphatidyl choline, generating lipid mediator lysophosphatidic acid. This report represents first crystal structure a member sphingomyelinase family laeta (SMase I), has been...
Tityus scorpion stings are an important public health problem in Brazil, where the incidence of such exceeds problems caused by other venomous animals, including snakes. In this study, we have analysed specific enzymatic activities venom from Brazilian scorpions genus, i.e., serrulatus, bahiensis and stigmurus. The data presented here revealed that spp. venoms exhibited significant hyaluronidase activity but no phospholipase activity. All samples ability to hydrolyse Abz-FLRRV-EDDnp...
Neutrophil dysfunction, resulting in inefficient bacterial clearance, is a feature of several serious medical conditions, including cystic fibrosis (CF) and sepsis. Poorly controlled neutrophil serine protease (NSP) activity complement activation have been implicated this phenomenon. The capacity for excess NSP secretion to influence the expression function important neutrophil-activating receptor C5aR was investigated. Purified NSPs cathepsin G (CG), elastase (NE), proteinase 3 cleaved 26-...
Cutaneous inoculation of Loxosceles spp. spider venoms produces local necrosis, occasionally accompanied by systemic intravascular clotting and hemolysis. In this work, we analyzed the role C system on lysis human erythrocytes (Eh) induced in vitro. Eh were treated with whole venom laeta, gaucho, or intermedia, purified proteins, incubated C-sufficient (Cs-NHS) C9-depleted autologous (C9d-NHS) serum. Hemolysis was determined spectrophotometrically, deposition components removal regulatory...
Summary We have recently shown that the sphingomyelinase toxins P1 and P2 from venom of spider Loxosceles intermedia induce complement (C)‐dependent lysis autologous erythrocytes by induction cleavage cell surface glycophorins through activation an endogenous metalloproteinase facilitating alternative pathway C. Phospholipase D (PLD) Corynebacterium pseudotuberculosis shows some degree homology with sphingomyelinases can similar clinical symptoms to those observed after envenomation. The aim...
Resistance to complement-mediated lysis in Trypanosoma cruzi is due the expression of complement-regulatory factors by virulent developmental forms this protozoan parasite. An 87- 93-kDa molecule, which we have termed T-DAF (trypomastigote decay-accelerating factor), present on surface parasite and inhibits complement activation a manner functionally similar mammalian regulatory component, factor. In report, characterized monospecific polyclonal monoclonal antibodies were obtained from mice...
Recently, nuclear distribution element-like (NUDEL) has been implicated to play a role in lissencephaly and schizophrenia through interactions with the gene 1 (Lis1) disrupted-in-schizophrenia (DISC1) products, respectively. Interestingly, NUDEL is same protein as endooligopeptidase A (EOPA), thiol-activated peptidase involved conversion inactivation of number bioactive peptides. In this study, we have cloned EOPA from human brain confirmed that it equivalent NUDEL, leading us suggest single...