A5035151105- Venomous Animal Envenomation and Studies
- Monoclonal and Polyclonal Antibodies Research
- Rabies epidemiology and control
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Pharmacological Effects of Natural Compounds
- Advanced Drug Delivery Systems
- Hepatitis B Virus Studies
- Escherichia coli research studies
- Viral Infectious Diseases and Gene Expression in Insects
- Heat shock proteins research
- Yersinia bacterium, plague, ectoparasites research
- Mesoporous Materials and Catalysis
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Computational Drug Discovery Methods
- Science and Education Research
- History of Medicine and Tropical Health
- Entomological Studies and Ecology
- Immune Response and Inflammation
- Fungal Infections and Studies
- Antimicrobial Peptides and Activities
- Ocular Diseases and Behçet’s Syndrome
- Leprosy Research and Treatment
Instituto Butantan
2013-2023
Universidade Federal de São Paulo
2014
Fundação de Amparo à Pesquisa do Estado de São Paulo
2014
Universidade de São Paulo
2005-2014
Universidade Federal de Juiz de Fora
2014
Adaptimmune (United Kingdom)
2010
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2010
Universidade Cidade de São Paulo
2005
Instituto Biológico
1976-1990
Inserm
1976-1977
Human respiratory syncytial virus (HRSV) is the major cause of lower tract infections in children under 5 years age and elderly, causing annual disease outbreaks during fall winter. Multiple lineages HRSVA HRSVB serotypes co-circulate within a single outbreak display strongly temporal pattern genetic variation, with replacement dominant genotypes occurring consecutive years. In present study we utilized phylogenetic methods to detect map sites subject adaptive evolution G protein HRSVB. A...
Adding to antibodies: Ordered mesoporous silicas such as SBA-15 may be used adjuvants carry, protect, and deliver entrapped antigens, thereby assuring efficient stimulation of antigen-presenting cells improving antibody responsiveness. Thus, related silica mesostructures seem promising nanosystems for vaccine delivery. Venom proteins from the Micrurus ibiboboca snake (see picture) were utilized in study.
Abstract The genetic regulation of acute inflammatory reaction (AIR) was studied by the method bidirectional selective breeding, used to produce a line mice giving maximal and minimal (AIR max AIR min, respectively). triggered subcutaneous injection neutral substrate (suspension polyacrylamide microbeads), measured leukocyte serum protein accumulation in exudate. two parameters are positively correlated present normal frequency distribution. highly genetically heterogeneous foundation...
In 2006, the first report of a nanostructured material as adjuvant was described establishing effectiveness ordered mesoporous SBA-15 silica an immune adjuvant. The present study evaluated capacity to modulate responsiveness High and Low responder mice immunized with BSA encapsulated/adsorbed in by intramuscular or oral route effect compared induced aluminum hydroxide salts emulsified Incomplete Freund These results demonstrate ability non-toxic nanoparticles increase immunogenicity repair...
Micrurus snake bites can cause death by muscle paralysis and respiratory arrest, few hours after envenomation. The specific treatment for coral envenomation is the intravenous application of heterologous antivenom and, in Brazil, it produced horse immunization with a mixture M. corallinus frontalis venoms, snakes that inhabit South Southeastern regions country. However, this might be inefficient, considering existence intra- inter-specific variations composition venoms. Therefore, aim...
Background Mesenchymal stem cells have prominent immune modulatory properties, which may clinical applications; however their major source, bone marrow, is of limited availability. On the other hand, mesenchymal derived from human exfoliated deciduous teeth (SHEDs) are readily accessible, but regulatory properties not been completely investigated. This study was designed, therefore, to evaluate SHEDs influence on DCs differentiation, maturation, ability activate T and expand CD4+Foxp3+...
Recently, nuclear distribution element-like (NUDEL) has been implicated to play a role in lissencephaly and schizophrenia through interactions with the gene 1 (Lis1) disrupted-in-schizophrenia (DISC1) products, respectively. Interestingly, NUDEL is same protein as endooligopeptidase A (EOPA), thiol-activated peptidase involved conversion inactivation of number bioactive peptides. In this study, we have cloned EOPA from human brain confirmed that it equivalent NUDEL, leading us suggest single...
Selective breeding has been performed in mice for their high or low antibody responsiveness to Salmonella antigens (Ag). Two noncross-reacting Salmonellae (S. typhimurium and S. oranienburg) have alternately used immunize successive generations order avoid interference of maternally transmitted antibody. Both carry two independent antigens: flagellar (f) somatic (s). two-way selections were carried out: one agglutinin response fAg the other sAg (Selection Ags). The Ag was also measured...
Replication Protein A (RPA), the major single stranded DNA binding protein in eukaryotes, is composed of three subunits and a fundamental player metabolism, participating replication, transcription, repair, damage response. In human pathogenic trypanosomatids, only limited studies have been performed on RPA-1 from Leishmania. Here, we silico, vitro vivo analysis Trypanosoma cruzi RPA-2 subunits. Although computational suggests similarities Ob-fold structures RPA T. compared with mammalian...
Snakebites are still a critical public health problem in developing countries or isolated areas. In Brazil, the North Region has high distribution coefficient worsened by significant number of eventually unreported cases, due to difficulties access services, natural geographic barriers and vast territory. Rio Negro area, species Bothrops atrox, brazili, Lachesis muta Bothriopsis taeniata thought be major responsible for snakebites. The aim this study was qualitatively quantitatively...
This work reports a detailed analysis of the ordered mesoporous SBA-15 silica synthesis procedure that provides matrix with mean pore diameter around 10 nm. The encapsulation bovine serum albumin (BSA) by four different methods allowed determination best imbibition condition, which is keeping mixture under rest and solvent evaporation. Simulation in situ SAXS scattered intensity BSA release potassium buffer solution, gastrointestinal fluids revealed slow evolution content, independent media....
Background The caterpillar of the moth Premolis semirufa (Lepidoptera: Arctiidae), commonly named Pararama, is endemic Amazon basin. Accidental contact with these bristles causes local symptoms such as intense heat, pain, edema and itching which last for three to seven days; however, after multiples contacts, it may induce joint-space narrowing bone alteration, well degeneration articular cartilage immobilization affected joints. Specific treatment this disease does not exist, but...
Neuropathic pain is a disease caused by structural and functional plasticity in central peripheral sensory pathways that produce alterations nociceptive processing. Currently, pharmacological treatment for this condition remains challenge. Crotoxin (CTX), the main neurotoxin of Crotalus durissus terrificus rattlesnake venom, has well described prolonged anti-inflammatory antinociceptive activities. In spite its potential benefits, toxicity CTX limiting factor use. SBA-15 an inert...
Abstract Developing a technology that enables oral vaccines to work efficiently remains considerable effort since number of difficulties must be addressed. The key objective being ensure the safe passage through harsh conditions within gastrointestinal tract, promoting delivery induces enhanced immune response. In particular case hepatitis B, formulation in nanostructured silica SBA-15 is viable approach. As result its porous structure, low toxicity and structural stability, capable protect...
The antibody responsiveness to and the specific vaccination effect of rabies virus infection were investigated in high- low-responder lines mice produced by two-way selective breedings for quantitative production antibodies flagellar (H/f L/f lines) or somatic (H/s L/s antigens salmonellae. After immunization, both high more resistant than low lines, protector was related level produced, as demonstrated neutralizing serum activity. present findings confirm nonspecific genetic modification...