A5056105184- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Acute Kidney Injury Research
- MicroRNA in disease regulation
- Chemotherapy-induced organ toxicity mitigation
- Artificial Intelligence in Healthcare
- Pancreatic function and diabetes
- Immunotherapy and Immune Responses
- Chronic Kidney Disease and Diabetes
- Cannabis and Cannabinoid Research
- Systemic Sclerosis and Related Diseases
- Diabetes and associated disorders
- Autophagy in Disease and Therapy
- Immune Response and Inflammation
- Cancer-related gene regulation
- Cardiovascular Disease and Adiposity
- COVID-19 Clinical Research Studies
- Gut microbiota and health
- Reproductive Health and Contraception
- Muscle and Compartmental Disorders
- Long-Term Effects of COVID-19
- Erythropoietin and Anemia Treatment
- Sinusitis and nasal conditions
- Diet and metabolism studies
- Cancer Cells and Metastasis
Universidade Federal de São Paulo
2014-2024
Santa Casa de Misericórdia de Passos
2024
Universidade de São Paulo
2011-2022
Universidade Federal de Viçosa
2017
University of Antwerp
2017
RWTH Aachen University
2016
Centro de Tecnologia Mineral
2013
Universidade Federal do Ceará
2013
Clinics Hospital of Ribeirão Preto
2011
Short-chain fatty acids (SCFAs) are fermentation end products produced by the intestinal microbiota and have anti-inflammatory histone deacetylase–inhibiting properties. Recently, a dual relationship between intestine kidneys has been unraveled. Therefore, we evaluated role of SCFA in an AKI model which inflammatory process detrimental role. We observed that therapy with three main SCFAs (acetate, propionate, butyrate) improved renal dysfunction caused injury. This protection was associated...
Adipose-derived mesenchymal stem cells (ADMSCs) display immunosuppressive properties, suggesting a promising therapeutic application in several autoimmune diseases, but their role type 1 diabetes (T1D) remains largely unexplored. The aim of this study was to investigate the immune regulatory properties allogeneic ADMSC therapy T cell–mediated NOD mice. treatment reversed hyperglycemia early-onset 78% diabetic mice, and effect associated with higher serum insulin, amylin, glucagon-like...
Butyrate is a short‐chain fatty acid derived from the metabolism of indigestible carbohydrates by gut microbiota. contributes to homeostasis, but it may also control inflammatory responses and host physiology in other tissues. inhibits histone deacetylases, thereby affecting gene transcription, signals through metabolite‐sensing G protein receptor (GPR)109a. We produced an mAb mouse GPR109a found high expression on podocytes kidney. Wild‐type Gpr109a –/– mice were induced develop nephropathy...
The antineoplastic drug cisplatin promotes renal injury, which limits its use. Protocols that reduce toxicity will allow higher doses to be used in treatment. Here, we compare physical exercise and caloric restriction (CR) as protocols injury mice. Male C57BL/6 were divided into four groups: control, cisplatin, + 30% CR cisplatin. Animals injected with a single dose of (20 mg/kg i.p.) sacrificed 96 h after injection. Quantitative real time PCR, histological analyses, immunohistochemistry,...
The antitumor effect of metformin has been demonstrated in several types cancer; however, the mechanisms involved are incompletely understood. In this study, we showed that acts directly on melanoma cells as well tumor microenvironment, particularly context immune response. vitro, induces a complex interplay between apoptosis and autophagy cells. anti-metastatic activity vivo was assessed mouse models challenged with B16F10 Metformin's was, part, system-dependent, whereas its properties were...
Background COVID-19 is a multisystemic disorder that frequently causes acute kidney injury (AKI). However, the precise clinical and biochemical variables associated with AKI progression in patients severe remain unclear. Methods We performed retrospective study on 278 hospitalized who were admitted to ward intensive care unit (ICU) between March 2020 June 2020, at University Hospital, São Paulo, Brazil. Patients aged ≥ 18 years confirmed RT-PCR included. was defined according Kidney Disease...
Adipose tissue-derived mesenchymal stem cells (ADSC) exhibit immunosuppressive capabilities both in vitro and vivo. Their use for therapy the transplant field is attractive as they could render of drugs unnecessary. The aim this study was to investigate effect ADSC on prolonging skin allograft survival. Animals that were treated with a single injection donor allogeneic one day after transplantation showed an increase graft survival by approximately week. This improvement associated preserved...
Standardization of mesenchymal stromal cells (MSCs) is hampered by the lack a precise definition for these cell preparations; example, there are no molecular markers to discern MSCs and fibroblasts. In this study, we followed hypothesis that specific DNA methylation (DNAm) patterns can assist classification MSCs. We utilized 190 DNAm profiles address impact tissue origin, donor age, replicative senescence, serum supplements on epigenetic makeup. Based this, elaborated simple signature based...
Acute kidney injury (AKI) is considered an inflammatory disease in which toll-like receptors (TLRs) signaling pathways play important role. The activation of TLRs results production several cytokines leading to further renal damage. In contrast, are key players on autophagy induction, associated with a protective function cisplatin-induced AKI. Hence, the present study aimed evaluate specific participation TLR2 and TLR4 molecules development Complementarily, we also investigated link between...
Mesenchymal stromal cells (MSCs) are multipotent with abilities to exert immunosuppressive response promoting tissue repair. Studies have shown that MSCs can secrete extracellular vesicles (MVs-MSCs) similar regulatory functions the parental cells. Furthermore, strong evidence suggesting MVs-MSCs modulate several immune (i.e., Th1, Th17, and Foxp3+ T cells). However, their precise effect on macrophages (Mϕs) remains unexplored. We investigated immunoregulatory of activated M1-Mϕs in vitro...
Background Mesenchymal stem cells have prominent immune modulatory properties, which may clinical applications; however their major source, bone marrow, is of limited availability. On the other hand, mesenchymal derived from human exfoliated deciduous teeth (SHEDs) are readily accessible, but regulatory properties not been completely investigated. This study was designed, therefore, to evaluate SHEDs influence on DCs differentiation, maturation, ability activate T and expand CD4+Foxp3+...
To evaluate the effect of SHED-CM on proliferation, differentiation, migration ability, cell death, gene expression and production VEGF HUVEC in vitro a rodent orthotopic dental pulp regeneration.Three culture media [M199, DMEM/Ham's F12 conditioned by SHEDs] were used as experimental groups. was prepared maintaining confluent cells without serum for 3 days. The proliferation death marker HUVECs assessed using flow cytometry. capacity formation vascular-like structures analyzed grown over...
Nephrotoxicity is substantial side effect for 30% of patients undergoing cancer therapy with cisplatin and may force them to change or even abandon the treatment. Studies regarding aerobic exercise have shown its efficacy treatment many types diseases capacity reduce tumors. However, little known about impact physical on cisplatin-induced acute kidney injury (AKI). In present study, our aim was investigate role in AKI induced by cisplatin. We submitted C57Bl6 male mice seven weeks chronic a...
Mesenchymal stromal cells (MSCs) can generate immunological tolerance due to their regulatory activity in many immune cells. Extracellular vesicles (EVs) release is a pivotal mechanism by which MSCs exert actions. In this study, we evaluate whether mesenchymal cell extracellular (MSC-EVs) modulate T response. were expanded and EVs obtained differential ultracentrifugation of the supernatant. The incorporation MSC-EVs was detected confocal microscopy. Expression surface markers flow cytometry...
Mesenchymal stromal cells (MSCs) orchestrate tissue repair by releasing cell-derived microvesicles (MVs), that presumably small RNA species, modulate global gene expression. The knowledge of miRNA/mRNA signatures linked to a reparative status may elucidate some the molecular events associated with MSC protection. Here, we used model cisplatin-induced kidney injury (AKI) assess how MSCs or MVs could restore function. and presented similar protective effects, which were evidenced in vivo vitro...
Adenine overload promotes intratubular crystal precipitation and interstitial nephritis. We showed recently that these abnormalities are strongly attenuated in mice knockout for Toll-like receptors-2, -4, MyD88, ASC, or caspase-1. now investigated whether NF-κB activation also plays a pathogenic role this model. Adult male Munich-Wistar rats were distributed among three groups: C (n = 17), receiving standard chow; ADE given adenine the chow at 0.7% 1 wk 0.5% 2 wk; + pyrrolidine...
Pattern recognition receptors (PRRs), such as Nod2, Nlrp3, Tlr2, Trl4, and Tlr9, are directly involved in type 1 diabetes (T1D) susceptibility. However, the role of cytosolic DNA sensor, AIM2, T1D pathogenesis is still unknown. Here, we demonstrate that C57BL/6 mice lacking AIM2 (AIM2−/−) prone to streptozotocin (STZ)-induced T1D, compared WT mice. The AIM2−/− phenotype associated with a greater proinflammatory response pancreatic tissues, alterations gut microbiota bacterial translocation...
Nasal polyposis is a severe, chronic inflammatory condition of the paranasal sinuses and frequently associated with asthma aspirin sensitivity. Mesenchymal stem cells exhibit potent immunosuppressive effect in several conditions, their role nasal remains little explored. Hence, we investigated whether bone marrow-derived mesenchymal could modulate cell phenotype polyp milieu. After coculture cells, frequency these was found to decrease. Furthermore, promoted strong inhibition CD4+ CD8+ T...